Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus

Detalhes bibliográficos
Autor(a) principal: Oliveira, Marcus Fernandes de
Data de Publicação: 2005
Outros Autores: Kycia, Stefan W, Gomez, Ariel, Kosar, Aaron J, Bohle, D Scott, Hempelmann, Ernst, Menezes, Diego Silva, Santos, Marcos André Vannier dos, Oliveira, Pedro Lagerblad de, Ferreira, Sérgio Teixeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1016/j.febslet.2005.09.035
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15115
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programas de Biologia Molecular e Biotecnologia, Bioquímica e Biofísica Celular. Rio de Janeiro, RJ, Brasil
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spelling Oliveira, Marcus Fernandes deKycia, Stefan WGomez, ArielKosar, Aaron JBohle, D ScottHempelmann, ErnstMenezes, Diego SilvaSantos, Marcos André Vannier dosOliveira, Pedro Lagerblad deFerreira, Sérgio Teixeira2016-07-25T14:25:37Z2016-07-25T14:25:37Z2005OLIVEIRA, M. F. et al. Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus. FEBS Letters, v. 579, p. 6010–6016, 2005.0014-5793https://www.arca.fiocruz.br/handle/icict/1511510.1016/j.febslet.2005.09.035engElsevierStructural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programas de Biologia Molecular e Biotecnologia, Bioquímica e Biofísica Celular. Rio de Janeiro, RJ, BrasilUniversity of Guelph, Guelph. Department of Physics, MacNaughton Bldg, Gordon Street. Guelph, Ont. CanadaUniversity of Guelph, Guelph. Department of Physics, MacNaughton Bldg, Gordon Street. Guelph, Ont., CanadaMcGill University. Department of Chemistry. Montreal, Que., CanadaMcGill University. Department of Chemistry. Montreal, Que., CanadaTubingen University. Institute of Tropical Medicine. Department of Parasitology. Tubingen, GermanyFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programas de Biologia Molecular e Biotecnologia, Bioquímica e Biofísica Celular. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programas de Biologia Molecular e Biotecnologia, Bioquímica e Biofísica Celular. Rio de Janeiro, RJ, Brasil / Laboratório Nacional de Luz Síncrotron. Campinas, SP, BrasilHemozoin (Hz) is a heme crystal produced upon the digestion of hemoglobin (Hb) by blood-feeding organisms as a main mechanism of heme disposal. The structure of Hz consists of heme dimers bound by reciprocal iron-carboxylate interactions and stabilized by hydrogen bonds. We have recently described heme crystals in the blood fluke, Schistosoma mansoni, and in the kissing bug, Rhodnius prolixus. Here, we characterized the structures and morphologies of the heme crystals from those two organisms and compared them to synthetic beta-hematin (betaH). Synchrotron radiation X-ray powder diffraction showed that all heme crystals share the same unit cell and structure. The heme crystals isolated from S. mansoni and R. prolixus consisted of very regular units assembled in multicrystalline spherical structures exhibiting remarkably distinct surface morphologies compared to betaH. In both organisms, Hz formation occurs inside lipid droplet-like particles or in close association to phospholipid membranes. These results show, for the first time, the structural and morphological characterization of natural Hz samples obtained from these two blood-feeding organisms. Moreover, Hz formation occurring in close association to a hydrophobic environment seems to be a common trend for these organisms and may be crucial to produce very regular shaped phases, allowing the formation of multicrystalline assemblies in the guts of S. mansoni and R. prolixus.HemozoinHemeX-ray diffractionRhodnius prolixusSchistosoma mansoniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15115/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALOliveira MF Structural and morphological......pdfOliveira MF Structural and morphological......pdfapplication/pdf426444https://www.arca.fiocruz.br/bitstream/icict/15115/2/Oliveira%20MF%20Structural%20and%20morphological......pdf7cef808ba81bac15d03cff11583556a5MD52TEXTOliveira MF Structural and morphological......pdf.txtOliveira MF Structural 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dc.title.pt_BR.fl_str_mv Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
title Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
spellingShingle Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
Oliveira, Marcus Fernandes de
Hemozoin
Heme
X-ray diffraction
Rhodnius prolixus
Schistosoma mansoni
title_short Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
title_full Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
title_fullStr Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
title_full_unstemmed Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
title_sort Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus
author Oliveira, Marcus Fernandes de
author_facet Oliveira, Marcus Fernandes de
Kycia, Stefan W
Gomez, Ariel
Kosar, Aaron J
Bohle, D Scott
Hempelmann, Ernst
Menezes, Diego Silva
Santos, Marcos André Vannier dos
Oliveira, Pedro Lagerblad de
Ferreira, Sérgio Teixeira
author_role author
author2 Kycia, Stefan W
Gomez, Ariel
Kosar, Aaron J
Bohle, D Scott
Hempelmann, Ernst
Menezes, Diego Silva
Santos, Marcos André Vannier dos
Oliveira, Pedro Lagerblad de
Ferreira, Sérgio Teixeira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Marcus Fernandes de
Kycia, Stefan W
Gomez, Ariel
Kosar, Aaron J
Bohle, D Scott
Hempelmann, Ernst
Menezes, Diego Silva
Santos, Marcos André Vannier dos
Oliveira, Pedro Lagerblad de
Ferreira, Sérgio Teixeira
dc.subject.en.pt_BR.fl_str_mv Hemozoin
Heme
X-ray diffraction
Rhodnius prolixus
Schistosoma mansoni
topic Hemozoin
Heme
X-ray diffraction
Rhodnius prolixus
Schistosoma mansoni
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programas de Biologia Molecular e Biotecnologia, Bioquímica e Biofísica Celular. Rio de Janeiro, RJ, Brasil
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2016-07-25T14:25:37Z
dc.date.available.fl_str_mv 2016-07-25T14:25:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, M. F. et al. Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus. FEBS Letters, v. 579, p. 6010–6016, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15115
dc.identifier.issn.pt_BR.fl_str_mv 0014-5793
dc.identifier.doi.none.fl_str_mv 10.1016/j.febslet.2005.09.035
identifier_str_mv OLIVEIRA, M. F. et al. Structural and morphological characterization of hemozoin produced by Schistosoma mansoni and Rhodnius prolixus. FEBS Letters, v. 579, p. 6010–6016, 2005.
0014-5793
10.1016/j.febslet.2005.09.035
url https://www.arca.fiocruz.br/handle/icict/15115
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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