A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes

Detalhes bibliográficos
Autor(a) principal: Chagas, Andrezza C.
Data de Publicação: 2013
Outros Autores: Calvo, Eric, Rios-Velásquez, Claudia M., Pessoa, Felipe A. C., Medeiros, Jansen F., Ribeiro, José M. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14950
https://doi.org/10.1186/1471-2164-14-875
Resumo: National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Rockville, MD, USA.
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spelling Chagas, Andrezza C.Calvo, EricRios-Velásquez, Claudia M.Pessoa, Felipe A. C.Medeiros, Jansen F.Ribeiro, José M. C.2016-07-14T15:28:27Z2016-07-15T18:40:39Z2016-07-14T15:28:27Z2016-07-15T18:40:39Z2013CHAGAS, Andrezza C. et al. A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes. BMC Genomics, v. 14, p. 1-19, 2013.https://www.arca.fiocruz.br/handle/icict/14950https://doi.org/10.1186/1471-2164-14-875engBMC GenomicsA deep insight into the sialotranscriptome of the mosquito, Psorophora albipesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Rockville, MD, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Rockville, MD, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Rockville, MD, USA.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Biodiversity Laboratory. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Biodiversity Laboratory. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Entomology Laboratory. Porto Velho, RO, Brasil.Background: Psorophora mosquitoes are exclusively found in the Americas and have been associated with transmission of encephalitis and West Nile fever viruses, among other arboviruses. Mosquito salivary glands represent the final route of differentiation and transmission of many parasites. They also secrete molecules with powerful pharmacologic actions that modulate host hemostasis, inflammation, and immune response. Here, we employed next generation sequencing and proteome approaches to investigate for the first time the salivary composition of a mosquito member of the Psorophora genus. We additionally discuss the evolutionary position of this mosquito genus into the Culicidae family by comparing the identity of its secreted salivary compounds to other mosquito salivary proteins identified so far. Results: Illumina sequencing resulted in 13,535,229 sequence reads, which were assembled into 3,247 contigs. All families were classified according to their in silico-predicted function/ activity. Annotation of these sequences allowed classification of their products into 83 salivary protein families, twenty (24.39%) of which were confirmed by our subsequent proteome analysis. Two protein families were deorphanized from Aedes and one from Ochlerotatus, while four protein families were described as novel to Psorophora genus because they had no match with any other known mosquito salivary sequence. Several protein families described as exclusive to Culicines were present in Psorophora mosquitoes, while we did not identify any member of the protein families already known as unique to Anophelines. Also, the Psorophora salivary proteins had better identity to homologs in Aedes (69.23%), followed by Ochlerotatus (8.15%), Culex (6.52%), and Anopheles (4.66%), respectively. Conclusions: This is the first sialome (from the Greek sialo = saliva) catalog of salivary proteins from a Psorophora mosquito, which may be useful for better understanding the lifecycle of this mosquito and the role of its salivary secretion in arboviral transmission.SialotranscriptomeMosquitoPsorophoraAlbipesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTA deep insight into.pdf.txtA deep insight into.pdf.txtExtracted texttext/plain75909https://www.arca.fiocruz.br/bitstream/icict/14950/4/A%20deep%20insight%20into.pdf.txt38505c38136d376d18397afc6214b30dMD54ORIGINALA deep insight into.pdfapplication/pdf7744357https://www.arca.fiocruz.br/bitstream/icict/14950/2/A%20deep%20insight%20into.pdff6a3b40ab1b92fe8641495d866899badMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14950/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/149502018-07-16 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dc.title.pt_BR.fl_str_mv A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
title A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
spellingShingle A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
Chagas, Andrezza C.
Sialotranscriptome
Mosquito
Psorophora
Albipes
title_short A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
title_full A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
title_fullStr A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
title_full_unstemmed A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
title_sort A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes
author Chagas, Andrezza C.
author_facet Chagas, Andrezza C.
Calvo, Eric
Rios-Velásquez, Claudia M.
Pessoa, Felipe A. C.
Medeiros, Jansen F.
Ribeiro, José M. C.
author_role author
author2 Calvo, Eric
Rios-Velásquez, Claudia M.
Pessoa, Felipe A. C.
Medeiros, Jansen F.
Ribeiro, José M. C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Chagas, Andrezza C.
Calvo, Eric
Rios-Velásquez, Claudia M.
Pessoa, Felipe A. C.
Medeiros, Jansen F.
Ribeiro, José M. C.
dc.subject.en.eng.fl_str_mv Sialotranscriptome
Mosquito
Psorophora
Albipes
topic Sialotranscriptome
Mosquito
Psorophora
Albipes
description National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Rockville, MD, USA.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-07-14T15:28:27Z
2016-07-15T18:40:39Z
dc.date.available.fl_str_mv 2016-07-14T15:28:27Z
2016-07-15T18:40:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHAGAS, Andrezza C. et al. A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes. BMC Genomics, v. 14, p. 1-19, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14950
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1186/1471-2164-14-875
identifier_str_mv CHAGAS, Andrezza C. et al. A deep insight into the sialotranscriptome of the mosquito, Psorophora albipes. BMC Genomics, v. 14, p. 1-19, 2013.
url https://www.arca.fiocruz.br/handle/icict/14950
https://doi.org/10.1186/1471-2164-14-875
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC Genomics
publisher.none.fl_str_mv BMC Genomics
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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