Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni

Detalhes bibliográficos
Autor(a) principal: Blauth, Monica L.
Data de Publicação: 2009
Outros Autores: Bruno, Rafaela V., Abdelhay, Eliana Saul Furquim Werneck, Loreto, Elgion L. S., Valente, Vera L. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30718
Resumo: Universidade do Estado de Mato Grosso. Departamento de Ciências Biológicas. Tangará da Serra, MT, Brasil / Universidade Federal do Rio Grande do Sul. Instituto de Biociências. Departamento de Genética. Laboratório de Drosophila. Porto Alegre, RS, Brasil.
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spelling Blauth, Monica L.Bruno, Rafaela V.Abdelhay, Eliana Saul Furquim WerneckLoreto, Elgion L. S.Valente, Vera L. S.2018-12-20T13:42:15Z2018-12-20T13:42:15Z2009BLAUTH, Monica L. et al. Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni. Anais da Academia Brasileira de Ciências, v. 81, n. 4, p. 679-689, 2009.0001-3765https://www.arca.fiocruz.br/handle/icict/3071810.1590/S0001-376520090004000071678-2690engAcademia Brasileira de CiênciasDisgenesia híbridaDrosophilaD. willistonihybrid dysgenesisRNAiP elementTransposable elementElementos de DNA TransponíveisInterferência de RNADrosophilaDetection of P element transcripts in embryos of Drosophila melanogaster and D. willistoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado de Mato Grosso. Departamento de Ciências Biológicas. Tangará da Serra, MT, Brasil / Universidade Federal do Rio Grande do Sul. Instituto de Biociências. Departamento de Genética. Laboratório de Drosophila. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Instos. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Centro de Transplante de Medula Óssea. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Instituto de Biociências. Departamento de Genética. Laboratório de Drosophila. Porto Alegre, RS, Brasil / Universidade Federal de Santa Maria. Centro de Ciências Naturais e Exatas. Departamento de Biologia. Santa Maria, RS, Brasil.Universidade Federal do Rio Grande do Sul. Instituto de Biociências. Departamento de Genética. Laboratório de Drosophila. Porto Alegre, RS, Brasil / Universidade Federal de Santa Maria. Centro de Ciências Naturais e Exatas. Departamento de Biologia. Santa Maria, RS, Brasil.The P element is one of the most thoroughly studied transposable elements (TE). Its mobilization causes the hybrid dysgenesis that was first described in Drosophila melanogaster. While studies of the P element have mainly been done in D. melanogaster, it is believed that Drosophila willistoni was the original host species of this TE and that P was transposed to the D. melanogaster genome by horizontal transfer. Our study sought to compare the transcriptional behavior of the P element in embryos of D. melanogaster, which is a recent host, with embryos of two strains of D. willistoni, a species that has contained the P element for a longer time. In both species, potential transcripts of transposase, the enzyme responsible for the TE mobilization, were detected, as were transcripts of the 66-kDa repressor, truncated and antisense sequences, which can have the ability to prevent TEs mobilization. The truncated transcripts reveal the truncated P elements present in the genome strains and whose number seems to be related to the invasion time of the genome by the TE. No qualitative differences in antisense transcripts were observed among the strains, even in the D. willistoni strain with the highest frequency of heterochromatic P elements.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/30718/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrafaelav_bruno_etal_IOC_2009.pdfrafaelav_bruno_etal_IOC_2009.pdfapplication/pdf1409373https://www.arca.fiocruz.br/bitstream/icict/30718/2/rafaelav_bruno_etal_IOC_2009.pdf39eef486097d649fce38ed8d35c046a1MD52TEXTrafaelav_bruno_etal_IOC_2009.pdf.txtrafaelav_bruno_etal_IOC_2009.pdf.txtExtracted texttext/plain44635https://www.arca.fiocruz.br/bitstream/icict/30718/3/rafaelav_bruno_etal_IOC_2009.pdf.txt605e0656e6433e2519daf60e7c936136MD53icict/307182020-12-03 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dc.title.pt_BR.fl_str_mv Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
title Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
spellingShingle Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
Blauth, Monica L.
Disgenesia híbrida
Drosophila
D. willistoni
hybrid dysgenesis
RNAi
P element
Transposable element
Elementos de DNA Transponíveis
Interferência de RNA
Drosophila
title_short Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
title_full Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
title_fullStr Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
title_full_unstemmed Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
title_sort Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni
author Blauth, Monica L.
author_facet Blauth, Monica L.
Bruno, Rafaela V.
Abdelhay, Eliana Saul Furquim Werneck
Loreto, Elgion L. S.
Valente, Vera L. S.
author_role author
author2 Bruno, Rafaela V.
Abdelhay, Eliana Saul Furquim Werneck
Loreto, Elgion L. S.
Valente, Vera L. S.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Blauth, Monica L.
Bruno, Rafaela V.
Abdelhay, Eliana Saul Furquim Werneck
Loreto, Elgion L. S.
Valente, Vera L. S.
dc.subject.other.pt_BR.fl_str_mv Disgenesia híbrida
topic Disgenesia híbrida
Drosophila
D. willistoni
hybrid dysgenesis
RNAi
P element
Transposable element
Elementos de DNA Transponíveis
Interferência de RNA
Drosophila
dc.subject.en.pt_BR.fl_str_mv Drosophila
D. willistoni
hybrid dysgenesis
RNAi
P element
Transposable element
dc.subject.decs.pt_BR.fl_str_mv Elementos de DNA Transponíveis
Interferência de RNA
Drosophila
description Universidade do Estado de Mato Grosso. Departamento de Ciências Biológicas. Tangará da Serra, MT, Brasil / Universidade Federal do Rio Grande do Sul. Instituto de Biociências. Departamento de Genética. Laboratório de Drosophila. Porto Alegre, RS, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-12-20T13:42:15Z
dc.date.available.fl_str_mv 2018-12-20T13:42:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BLAUTH, Monica L. et al. Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni. Anais da Academia Brasileira de Ciências, v. 81, n. 4, p. 679-689, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30718
dc.identifier.issn.pt_BR.fl_str_mv 0001-3765
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0001-37652009000400007
dc.identifier.eissn.none.fl_str_mv 1678-2690
identifier_str_mv BLAUTH, Monica L. et al. Detection of P element transcripts in embryos of Drosophila melanogaster and D. willistoni. Anais da Academia Brasileira de Ciências, v. 81, n. 4, p. 679-689, 2009.
0001-3765
10.1590/S0001-37652009000400007
1678-2690
url https://www.arca.fiocruz.br/handle/icict/30718
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30718/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/30718/2/rafaelav_bruno_etal_IOC_2009.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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