In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil

Detalhes bibliográficos
Autor(a) principal: Pandolfi, Rafael
Data de Publicação: 2017
Outros Autores: Almeida, Denise Ramos de, Pinto, Marcelo Alves, Kreutz, Luiz Carlos, Frandoloso, Rafael
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24847
Resumo: Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil.
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spelling Pandolfi, RafaelAlmeida, Denise Ramos dePinto, Marcelo AlvesKreutz, Luiz CarlosFrandoloso, Rafael2018-02-15T16:49:54Z2018-02-15T16:49:54Z2017PANDOLFI, Rafael; et al. In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG antihepatitis E virus amongst blood donors in south Brazil. Plos One, v.12, n.5, e017640, 12p, May 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2484710.1371/journal.pone.0176409engPublic Library of ScienceVírus da Hepatite EDoadores de sangueSul do BrasilImunoglobulina GTeste ELIZAAnticorposproteína ORF2 recombinanteHepatitis E Virusblood donorssouth BrazilELIZAIgG antihepatitis E virusantibodiesrecombinant ORF2 proteinIn house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil.Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil.Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil.Hepatitis E Virus (HEV) is a zoonotic pathogen responsible for causing acute hepatitis in human, especially in developing countries. Diagnosis of HEV usually relies on the detection of antibodies mostly by enzyme-linked immunosorbent assay (ELISA). In the present study, we designed a new indirect ELISA (iELISA) based on a short recombinant peptide derived from the capsid protein (ORF2p) and demonstrated its potential for detecting human IgG against HEV genotype 3. The best polystyrene plate (Maxisorp®), optimal ORF2p coating antigen concentration (0,67μg/well) and primary antibody dilution (1:100) were determined. This iELISA showed a sensitivity of 91.4% and specificity of 95.9%. The comparison of our in house iELISA with a commercial assay (RecomWell, Mikrogen®) showed 94.25% of agreement and a kappa index of 0.88. The ORF2 recombinant ELISA was used to screen 780 blood donors for anti-HEV IgG and we found that 314 (40,25%) of these donors were IgG positive. This high prevalence of antibodies suggests, for the first time, that the Southern Brazil region might be endemic to Hepatitis E Virus genotype 3.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24847/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarceloalves_pinto_etal_IOC_2017.pdfmarceloalves_pinto_etal_IOC_2017.pdfapplication/pdf2104206https://www.arca.fiocruz.br/bitstream/icict/24847/2/marceloalves_pinto_etal_IOC_2017.pdfd20f4b58077299faab639908c1fd97dfMD52TEXTmarceloalves_pinto_etal_IOC_2017.pdf.txtmarceloalves_pinto_etal_IOC_2017.pdf.txtExtracted texttext/plain39132https://www.arca.fiocruz.br/bitstream/icict/24847/3/marceloalves_pinto_etal_IOC_2017.pdf.txtb01ed37f73844b27675ea108a48fec71MD53icict/248472018-08-15 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dc.title.pt_BR.fl_str_mv In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
title In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
spellingShingle In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
Pandolfi, Rafael
Vírus da Hepatite E
Doadores de sangue
Sul do Brasil
Imunoglobulina G
Teste ELIZA
Anticorpos
proteína ORF2 recombinante
Hepatitis E Virus
blood donors
south Brazil
ELIZA
IgG antihepatitis E virus
antibodies
recombinant ORF2 protein
title_short In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
title_full In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
title_fullStr In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
title_full_unstemmed In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
title_sort In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil
author Pandolfi, Rafael
author_facet Pandolfi, Rafael
Almeida, Denise Ramos de
Pinto, Marcelo Alves
Kreutz, Luiz Carlos
Frandoloso, Rafael
author_role author
author2 Almeida, Denise Ramos de
Pinto, Marcelo Alves
Kreutz, Luiz Carlos
Frandoloso, Rafael
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pandolfi, Rafael
Almeida, Denise Ramos de
Pinto, Marcelo Alves
Kreutz, Luiz Carlos
Frandoloso, Rafael
dc.subject.other.pt_BR.fl_str_mv Vírus da Hepatite E
Doadores de sangue
Sul do Brasil
Imunoglobulina G
Teste ELIZA
Anticorpos
proteína ORF2 recombinante
topic Vírus da Hepatite E
Doadores de sangue
Sul do Brasil
Imunoglobulina G
Teste ELIZA
Anticorpos
proteína ORF2 recombinante
Hepatitis E Virus
blood donors
south Brazil
ELIZA
IgG antihepatitis E virus
antibodies
recombinant ORF2 protein
dc.subject.en.pt_BR.fl_str_mv Hepatitis E Virus
blood donors
south Brazil
ELIZA
IgG antihepatitis E virus
antibodies
recombinant ORF2 protein
description Universidade de Passo Fundo. Faculdade de Agronomia e Medicina Veterinária. Laboratório de Microbiologia e Imunologia Avançada. Passo Fundo, RS, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-15T16:49:54Z
dc.date.available.fl_str_mv 2018-02-15T16:49:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PANDOLFI, Rafael; et al. In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG antihepatitis E virus amongst blood donors in south Brazil. Plos One, v.12, n.5, e017640, 12p, May 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24847
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0176409
identifier_str_mv PANDOLFI, Rafael; et al. In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG antihepatitis E virus amongst blood donors in south Brazil. Plos One, v.12, n.5, e017640, 12p, May 2017.
1932-6203
10.1371/journal.pone.0176409
url https://www.arca.fiocruz.br/handle/icict/24847
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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