Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48256 |
Resumo: | Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil. |
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Pina, Jeferson Rodrigo SouzaSilva, João Victor SilvaCarvalho, Josiwander MirandaBitencourt, Heriberto RodriguesWatanabe, Luciano AlmeidaFernandes, Juan Matheus PereiraSouza, Guilherme Eduardo deAguiar, Anna Caroline CamposGuido, Rafael Victorio CarvalhoSouza, Fernando AlmeidaCalabrese, Kátia da SilvaMarinho, Patrícia Santana BarbosaMarinho, Andrey Moacir do Rosario2021-07-16T13:52:56Z2021-07-16T13:52:56Z2021PINA, Jeferson Rodrigo Souza et al. Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum. Molecules, v. 26, 3339, 11 p, 2021.1420-3049https://www.arca.fiocruz.br/handle/icict/4825610.3390/molecules26113339engMDPIAntimicrobianoAntiprotozoárioPoliketídeosXanthoneÁcido Graxo Sintase Tipo IIAntimicrobialAntiprotozoanPolyketidesFungiXanthoneÁcido Graxo Sintase Tipo IIAntiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Maranhão. Programa de Pós-Graduação em Ciências Animal. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil.The natural compound ravenelin was isolated from the biomass extracts of Exserohilum rostratum fungus, and its antimicrobial, antiplasmodial, and trypanocidal activities were evaluated. Ravenelin was isolated by column chromatography and HPLC and identified by NMR and MS. The susceptibility of Gram-positive and Gram-negative bacteria strains to ravenelin was determined by microbroth dilution assay. Cytotoxicity was evaluated in hepatocarcinoma cells (HepG2) and BALB/c peritoneal macrophages by using MTT. SYBR Green I-based assay was used in the asexual stages of Plasmodium falciparum. Trypanocidal activity was tested against the epimastigote and intracellular amastigote forms of Trypanosoma cruzi. Ravenelin was active against Gram-positive bacteria strains, with emphasis on Bacillus subtilis (MIC value of 7.5 µM). Ravenelin’s antiparasitic activities were assessed against both the epimastigote (IC50 value of 5 ± 1 µM) and the intracellular amastigote forms of T. cruzi (IC50 value of 9 ± 2 µM), as well as against P. falciparum (IC50 value of 3.4 ± 0.4 µM). Ravenelin showed low cytotoxic effects on both HepG2 (CC50 > 50 µM) and peritoneal macrophage (CC50 = 185 ± 1 µM) cells with attractive selectivity for the parasites (SI values > 15). These findings indicate that ravenelin is a natural compound with both antibacterial and antiparasitic activities, and considerable selectivity indexes. Therefore, ravenelin is an attractive candidate for hit-to-lead development.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48256/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFernandoSouza_JoaoVitorSilva_etal_IOC_2021.pdfFernandoSouza_JoaoVitorSilva_etal_IOC_2021.pdfapplication/pdf1022776https://www.arca.fiocruz.br/bitstream/icict/48256/2/FernandoSouza_JoaoVitorSilva_etal_IOC_2021.pdfb89e688d6a0fd6647d6f3eb64ac95e6bMD52TEXTFernandoSouza_JoaoVitorSilva_etal_IOC_2021.pdf.txtFernandoSouza_JoaoVitorSilva_etal_IOC_2021.pdf.txtExtracted texttext/plain42842https://www.arca.fiocruz.br/bitstream/icict/48256/3/FernandoSouza_JoaoVitorSilva_etal_IOC_2021.pdf.txt65a6ac0c6119d5a0d0fb07126c53b088MD53icict/482562023-09-05 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| dc.title.pt_BR.fl_str_mv |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum |
| title |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum |
| spellingShingle |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum Pina, Jeferson Rodrigo Souza Antimicrobiano Antiprotozoário Poliketídeos Xanthone Ácido Graxo Sintase Tipo II Antimicrobial Antiprotozoan Polyketides Fungi Xanthone Ácido Graxo Sintase Tipo II |
| title_short |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum |
| title_full |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum |
| title_fullStr |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum |
| title_full_unstemmed |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum |
| title_sort |
Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum |
| author |
Pina, Jeferson Rodrigo Souza |
| author_facet |
Pina, Jeferson Rodrigo Souza Silva, João Victor Silva Carvalho, Josiwander Miranda Bitencourt, Heriberto Rodrigues Watanabe, Luciano Almeida Fernandes, Juan Matheus Pereira Souza, Guilherme Eduardo de Aguiar, Anna Caroline Campos Guido, Rafael Victorio Carvalho Souza, Fernando Almeida Calabrese, Kátia da Silva Marinho, Patrícia Santana Barbosa Marinho, Andrey Moacir do Rosario |
| author_role |
author |
| author2 |
Silva, João Victor Silva Carvalho, Josiwander Miranda Bitencourt, Heriberto Rodrigues Watanabe, Luciano Almeida Fernandes, Juan Matheus Pereira Souza, Guilherme Eduardo de Aguiar, Anna Caroline Campos Guido, Rafael Victorio Carvalho Souza, Fernando Almeida Calabrese, Kátia da Silva Marinho, Patrícia Santana Barbosa Marinho, Andrey Moacir do Rosario |
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author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pina, Jeferson Rodrigo Souza Silva, João Victor Silva Carvalho, Josiwander Miranda Bitencourt, Heriberto Rodrigues Watanabe, Luciano Almeida Fernandes, Juan Matheus Pereira Souza, Guilherme Eduardo de Aguiar, Anna Caroline Campos Guido, Rafael Victorio Carvalho Souza, Fernando Almeida Calabrese, Kátia da Silva Marinho, Patrícia Santana Barbosa Marinho, Andrey Moacir do Rosario |
| dc.subject.other.pt_BR.fl_str_mv |
Antimicrobiano Antiprotozoário Poliketídeos Xanthone Ácido Graxo Sintase Tipo II |
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Antimicrobiano Antiprotozoário Poliketídeos Xanthone Ácido Graxo Sintase Tipo II Antimicrobial Antiprotozoan Polyketides Fungi Xanthone Ácido Graxo Sintase Tipo II |
| dc.subject.en.pt_BR.fl_str_mv |
Antimicrobial Antiprotozoan Polyketides Fungi Xanthone |
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Ácido Graxo Sintase Tipo II |
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Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil. |
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2021 |
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2021-07-16T13:52:56Z |
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2021-07-16T13:52:56Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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PINA, Jeferson Rodrigo Souza et al. Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum. Molecules, v. 26, 3339, 11 p, 2021. |
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1420-3049 |
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10.3390/molecules26113339 |
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PINA, Jeferson Rodrigo Souza et al. Antiprotozoal and Antibacterial Activity of Ravenelin, a Xanthone Isolated from the Endophytic Fungus Exserohilum rostratum. Molecules, v. 26, 3339, 11 p, 2021. 1420-3049 10.3390/molecules26113339 |
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