Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2

Detalhes bibliográficos
Autor(a) principal: Monteiro, Andrea de Souza
Data de Publicação: 2012
Outros Autores: Domingues, Vitor Souza, Souza, Marcus Vinícius Dias, Lula, Ivana, Gonçalves, Daniel Bonoto, Siqueira Filho, Ezequias Pessoa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7798
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Universidade Vale do Rio Doce Faculdade de Ciências da Saúde. Governador Valadares. Laboratório de Pesquisa em Microbiologia. MG, Brazil
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spelling Monteiro, Andrea de SouzaDomingues, Vitor SouzaSouza, Marcus Vinícius DiasLula, IvanaGonçalves, Daniel BonotoSiqueira Filho, Ezequias Pessoa de2014-06-05T13:53:39Z2014-06-05T13:53:39Z2012MONTEIRO, Andrea de Souza et al. Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2. Biotechnology for Biofuels. 2012, vol.5, pp. 29.754-6834https://www.arca.fiocruz.br/handle/icict/779810.1186/1754-6834-5-29engBioMed Central Ltd.Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Universidade Vale do Rio Doce Faculdade de Ciências da Saúde. Governador Valadares. Laboratório de Pesquisa em Microbiologia. MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrazilUniversidade Vale do Rio Doce Faculdade de Ciências da Saúde. Governador Valadares. Laboratório de Pesquisa em Microbiologia. MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Exatas e Tecnológicas. Departamento de Química. Laboratório de Encapsulamento Molecular e Biomateriais. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Exatas e Tecnológicas. Departamento de Química. Laboratório de Ressonância Magnética Nuclear. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais.Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Universidade Federal de São João Del-Rei. Laboratório de Processos Bioquímicos. São João Del Rey, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrazilBackground The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes. Results Trichosporon mycotoxinivorans CLA2 is bioemulsifier-producing yeast strain isolated from effluents of the dairy industry, with ability to emulsify different hydrophobic substrates. Bioemulsifier production (mg/L) and the emulsifying activity (E24) of this strain were optimized by response surface methodology using mineral minimal medium containing refinery waste as the carbon source, which consisted of diatomaceous earth impregnated with esters from filters used in biodiesel purification. The highest bioemulsifier production occurred in mineral minimal medium containing 75 g/L biodiesel residue and 5 g/L ammonium sulfate. The highest emulsifying activity was obtained in medium containing 58 g/L biodiesel refinery residue and 4.6 g/L ammonium sulfate, and under these conditions, the model estimated an emulsifying activity of 85%. Gas chromatography and mass spectrometry analysis suggested a bioemulsifier molecule consisting of monosaccharides, predominantly xylose and mannose, and a long chain aliphatic groups composed of octadecanoic acid and hexadecanoic acid at concentrations of 48.01% and 43.16%, respectively. The carbohydrate composition as determined by GC-MS of their alditol acetate derivatives showed a larger ratio of xylose (49.27%), mannose (39.91%), and glucose (10.81%). 1 H NMR spectra confirmed by COSY suggested high molecular weight, polymeric pattern, presence of monosaccharide’s and long chain aliphatic groups in the bioemulsifier molecule. Conclusions The biodiesel residue is an economical substrate, therefore seems to be very promising for the low-cost production of active emulsifiers in the emulsification of aromatics, aliphatic hydrocarbons, and kerosene.BioemulsifierYeastsResponse surface methodologyBiodiesel residueDiatomaceous earthinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2 - 1754-6834-5-29.pdfBioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2 - 1754-6834-5-29.pdfapplication/pdf2886084https://www.arca.fiocruz.br/bitstream/icict/7798/1/Bioconversion%20of%20biodiesel%20refinery%20waste%20in%20the%20bioemulsifier%20by%20Trichosporon%20mycotoxinivorans%20CLA2%20-%201754-6834-5-29.pdfe6c6a68b9130d3056572618e86f0c570MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7798/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2 - 1754-6834-5-29.pdf.txtBioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2 - 1754-6834-5-29.pdf.txtExtracted texttext/plain12https://www.arca.fiocruz.br/bitstream/icict/7798/5/Bioconversion%20of%20biodiesel%20refinery%20waste%20in%20the%20bioemulsifier%20by%20Trichosporon%20mycotoxinivorans%20CLA2%20-%201754-6834-5-29.pdf.txt36c6f0b2061da514c400c0bc2749b5cfMD55THUMBNAILBioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2 - 1754-6834-5-29.pdf.jpgBioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2 - 1754-6834-5-29.pdf.jpgGenerated Thumbnailimage/jpeg2110https://www.arca.fiocruz.br/bitstream/icict/7798/4/Bioconversion%20of%20biodiesel%20refinery%20waste%20in%20the%20bioemulsifier%20by%20Trichosporon%20mycotoxinivorans%20CLA2%20-%201754-6834-5-29.pdf.jpg9b1fbde5c7c049eefc38f6f37371b61aMD54icict/77982018-04-06 08:43:54.198oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
title Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
spellingShingle Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
Monteiro, Andrea de Souza
Bioemulsifier
Yeasts
Response surface methodology
Biodiesel residue
Diatomaceous earth
title_short Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
title_full Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
title_fullStr Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
title_full_unstemmed Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
title_sort Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2
author Monteiro, Andrea de Souza
author_facet Monteiro, Andrea de Souza
Domingues, Vitor Souza
Souza, Marcus Vinícius Dias
Lula, Ivana
Gonçalves, Daniel Bonoto
Siqueira Filho, Ezequias Pessoa de
author_role author
author2 Domingues, Vitor Souza
Souza, Marcus Vinícius Dias
Lula, Ivana
Gonçalves, Daniel Bonoto
Siqueira Filho, Ezequias Pessoa de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Monteiro, Andrea de Souza
Domingues, Vitor Souza
Souza, Marcus Vinícius Dias
Lula, Ivana
Gonçalves, Daniel Bonoto
Siqueira Filho, Ezequias Pessoa de
dc.subject.en.pt_BR.fl_str_mv Bioemulsifier
Yeasts
Response surface methodology
Biodiesel residue
Diatomaceous earth
topic Bioemulsifier
Yeasts
Response surface methodology
Biodiesel residue
Diatomaceous earth
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil/Universidade Vale do Rio Doce Faculdade de Ciências da Saúde. Governador Valadares. Laboratório de Pesquisa em Microbiologia. MG, Brazil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-06-05T13:53:39Z
dc.date.available.fl_str_mv 2014-06-05T13:53:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MONTEIRO, Andrea de Souza et al. Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2. Biotechnology for Biofuels. 2012, vol.5, pp. 29.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7798
dc.identifier.issn.none.fl_str_mv 754-6834
dc.identifier.doi.none.fl_str_mv 10.1186/1754-6834-5-29
identifier_str_mv MONTEIRO, Andrea de Souza et al. Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2. Biotechnology for Biofuels. 2012, vol.5, pp. 29.
754-6834
10.1186/1754-6834-5-29
url https://www.arca.fiocruz.br/handle/icict/7798
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central Ltd.
publisher.none.fl_str_mv BioMed Central Ltd.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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