The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59561 |
Resumo: | Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil. |
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Avelar, Bethânia Alves deLélis, Felipe J. N.Avelar, Renato SathlerWeber, MathiasFagundes, Elaine Maria de SouzaLopes, Miriam Teresa PazMartins Filho, Olindo AssisMelo, Gustavo Eustáquio Brito Alvim de2023-07-13T13:25:12Z2023-07-13T13:25:12Z2011AVELAR, Bethânia Alves et al. The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4+ T lymphocytes. Revista Brasileira de Farmacognosia, v. 21, n. 4, p. 662-667, Jul./Aug. 2011.0102-695Xhttps://www.arca.fiocruz.br/handle/icict/5956110.1590/S0102-695X2011005000096engSociedade Brasileira de FarmacognosiaThe crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores. Belo Horizonte, MG, Brazil.Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biomarcadores. Belo Horizonte, MG, Brazil.Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil.The plants of the Euphorbiaceae family, especially those of the genus Euphorbia, are frequently used by Brazilian folk communities to treat a wide variety of infectious, tumoral and inflammatory illnesses. Among the species of this genus, Euphorbia tirucalli L. is widely used in some Brazilian regions, such as the Jequitinhonha River Valley. There is evidence that the latex produced by E. tirucalli has antiviral and antitumor activities, but little is known about the mechanisms involved in these effects. It is likely that the mechanism for such activities involves leukocyte activation and cytokine production. In this work, we aimed to evaluate the production of type 1 (TNF-alpha and IFN-gamma) and type 2 (IL-4 and IL-10) cytokines by circulating leukocyte subsets submitted to brief stimulation with the crude latex of E. tirucalli. Peripheral blood leukocytes of twenty healthy subjects were submitted to 4 h incubation with crude E. tirucalli latex diluted in dimethylsulfoxide. After the incubation period, the cells were stained with FITC-conjugated monoclonal antibodies specific to the cell surface receptors CD4, CD8 and CD14, and to PE-conjugated monoclonal antibodies specific to the cytokines TNF-alpha, IFN-gamma, IL-4 and IL-10. The acquisition and analysis of data were performed by flow cytometry. The results showed a significant increase (p<0.05) in the percentage of CD4(+) T lymphocytes positive for the type 1 cytokines TNF-alpha and IFN-gamma. Neutrophils and CD8(+) T lymphocytes showed a mixed profile of cytokine production, characterized by an increase in the percentage of cells expressing IFN-gamma, TNF-alpha, and IL-10. The data indicate a predominant type 1 cytokine response. The findings presented suggest that the effect popularly attributed to E. tirucalli usage may be attributed to its effect on the production of TNF-alpha and IFN-gamma. However, the relationship between the in vitro and in vivo effects of E. tirucalli needs to be investigated.Euphorbia tirucalliMedicinal plantType 1 cytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALArticle 14.indd.pdfArticle 14.indd.pdfapplication/pdf424420https://www.arca.fiocruz.br/bitstream/icict/59561/2/Article%2014.indd.pdf51a3b74e7b03145329b3acd92f97c704MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59561/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/595612023-07-13 10:49:26.161oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-13T13:49:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.en_US.fl_str_mv |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes |
title |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes |
spellingShingle |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes Avelar, Bethânia Alves de Euphorbia tirucalli Medicinal plant Type 1 cytokines |
title_short |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes |
title_full |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes |
title_fullStr |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes |
title_full_unstemmed |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes |
title_sort |
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4(+) T lymphocytes |
author |
Avelar, Bethânia Alves de |
author_facet |
Avelar, Bethânia Alves de Lélis, Felipe J. N. Avelar, Renato Sathler Weber, Mathias Fagundes, Elaine Maria de Souza Lopes, Miriam Teresa Paz Martins Filho, Olindo Assis Melo, Gustavo Eustáquio Brito Alvim de |
author_role |
author |
author2 |
Lélis, Felipe J. N. Avelar, Renato Sathler Weber, Mathias Fagundes, Elaine Maria de Souza Lopes, Miriam Teresa Paz Martins Filho, Olindo Assis Melo, Gustavo Eustáquio Brito Alvim de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Avelar, Bethânia Alves de Lélis, Felipe J. N. Avelar, Renato Sathler Weber, Mathias Fagundes, Elaine Maria de Souza Lopes, Miriam Teresa Paz Martins Filho, Olindo Assis Melo, Gustavo Eustáquio Brito Alvim de |
dc.subject.en.en_US.fl_str_mv |
Euphorbia tirucalli Medicinal plant Type 1 cytokines |
topic |
Euphorbia tirucalli Medicinal plant Type 1 cytokines |
description |
Universidade Federal dos Vales do Jequitinhonha e Mucuri. Laboratório de Imunologia. Diamantina, MG, Brazil. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2023-07-13T13:25:12Z |
dc.date.available.fl_str_mv |
2023-07-13T13:25:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
AVELAR, Bethânia Alves et al. The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4+ T lymphocytes. Revista Brasileira de Farmacognosia, v. 21, n. 4, p. 662-667, Jul./Aug. 2011. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/59561 |
dc.identifier.issn.en_US.fl_str_mv |
0102-695X |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0102-695X2011005000096 |
identifier_str_mv |
AVELAR, Bethânia Alves et al. The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4+ T lymphocytes. Revista Brasileira de Farmacognosia, v. 21, n. 4, p. 662-667, Jul./Aug. 2011. 0102-695X 10.1590/S0102-695X2011005000096 |
url |
https://www.arca.fiocruz.br/handle/icict/59561 |
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eng |
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eng |
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Sociedade Brasileira de Farmacognosia |
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Sociedade Brasileira de Farmacognosia |
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