Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14673 |
Resumo: | Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratório de Mosquitos Genéticamente Modificados. São. Paulo, SP, Brasil. |
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Kojin, Bianca B.Silva, André Luis Costa daMaciel, CeresHenriques, Dayane AlvesCarvalho, Danilo O.Martin, KelcieMarinotti, OsvaldoJames, Anthony A.Bonaldo, Myrna C.Capurro, Margareth Lara2016-07-02T18:45:00Z2016-07-07T12:13:20Z2016-07-02T18:45:00Z2016-07-07T12:13:20Z2016KOJIN, Bianca B. et al. Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands. Malaria Journal, v.15:153, 11p, 2016.1475-2875https://www.arca.fiocruz.br/handle/icict/1467310.1186/s12936-016-1207-81475-2875engBioMed CentralEndogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glandsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratório de Mosquitos Genéticamente Modificados. São. Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratório de Mosquitos Genéticamente Modificados. São. Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratório de Mosquitos Genéticamente Modificados. São. Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. BSL3+ Laboratório. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratório de Mosquitos Genéticamente Modificados. São. Paulo, SP, Brasil.University of California Irvine. Department of Molecular Biology and Biochemistry. Irvine, CA, USA.University of California Irvine. Department of Molecular Biology and Biochemistry. Irvine, CA, USA.University of California Irvine. Department of Molecular Biology and Biochemistry. Irvine, CA, USA / University of California Irvine. Department of Microbiology and Molecular Genetics. Irvine, CA, USA.Fundação Oswaldo Cruz. instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivirus. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratório de Mosquitos Genéticamente Modificados. São. Paulo, SP, Brasil.Background: The circumsporozoite protein is the most abundant polypeptide expressed by sporozoites, the malaria parasite stage capable of infecting humans. Sporozoite invasion of mosquito salivary glands prior to transmission is likely mediated by a receptor/ligand-like interaction of the parasites with the target tissues, and the amino (NH2)terminal portion of CSP is involved in this interaction but not the TSR region on the carboxyl (C)-terminus. Peptides based on the NH2-terminal domain could compete with the parasites for the salivary gland receptors and thus inhibit penetration. Methods: Peptides based on the NH2-terminus and TSR domains of the CSP from avian or human malaria para‑ sites, Plasmodium gallinaceum and Plasmodium falciparum, respectively, were expressed endogenously in mosquito haemolymph using a transient (Sindbis virus-mediated) or stable (piggyBac-mediated transgenesis) system. Results: Transient endogenous expression of partial NH2-terminus peptide from P. falciparum CSP in P. gallinaceum-infected Aedes aegypti resulted in a reduced number of sporozoites in the salivary glands. When a transgenic approach was used to express a partial CSP NH2-terminal domain from P. gallinaceum the number of sporozoites in the salivary glands did not show a difference when compared to controls. However, a significant difference could be observed when mosquitoes with a lower infection were analysed. The same result could not be observed with mos‑ quitoes endogenously expressing peptides based on the TSR domain from either P. gallinaceum or P. falciparum. Conclusion: These results support the conclusion that CSP partial NH2-terminal domain can be endogenously expressed to promote a competition for the receptor used by sporozoites to invade salivary glands, and they could be used to block this interaction and reduce parasite transmission. The same effect cannot be obtained with peptides based on the TSR domain.Plasmodium gallinaceumPlasmodium falciparumCircumsporozoite proteinSindbis virusTransgenic mosquitoesAedes aegyptiPlasmodium falciparumAedes aegyptiVírus SindbisPlasmodium gallinaceuminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTMyrna2_bonaldo_etal_IOC-2016.pdf.txtMyrna2_bonaldo_etal_IOC-2016.pdf.txtExtracted texttext/plain44664https://www.arca.fiocruz.br/bitstream/icict/14673/4/Myrna2_bonaldo_etal_IOC-2016.pdf.txtf3a745b49ab4d5710850e46994f141b3MD54ORIGINALMyrna2_bonaldo_etal_IOC-2016.pdfapplication/pdf1664029https://www.arca.fiocruz.br/bitstream/icict/14673/2/Myrna2_bonaldo_etal_IOC-2016.pdfe2a8801d652e7f521f4b805766ea42beMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14673/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/146732022-06-24 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| dc.title.pt_BR.fl_str_mv |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands |
| title |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands |
| spellingShingle |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands Kojin, Bianca B. Plasmodium gallinaceum Plasmodium falciparum Circumsporozoite protein Sindbis virus Transgenic mosquitoes Aedes aegypti Plasmodium falciparum Aedes aegypti Vírus Sindbis Plasmodium gallinaceum |
| title_short |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands |
| title_full |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands |
| title_fullStr |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands |
| title_full_unstemmed |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands |
| title_sort |
Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands |
| author |
Kojin, Bianca B. |
| author_facet |
Kojin, Bianca B. Silva, André Luis Costa da Maciel, Ceres Henriques, Dayane Alves Carvalho, Danilo O. Martin, Kelcie Marinotti, Osvaldo James, Anthony A. Bonaldo, Myrna C. Capurro, Margareth Lara |
| author_role |
author |
| author2 |
Silva, André Luis Costa da Maciel, Ceres Henriques, Dayane Alves Carvalho, Danilo O. Martin, Kelcie Marinotti, Osvaldo James, Anthony A. Bonaldo, Myrna C. Capurro, Margareth Lara |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Kojin, Bianca B. Silva, André Luis Costa da Maciel, Ceres Henriques, Dayane Alves Carvalho, Danilo O. Martin, Kelcie Marinotti, Osvaldo James, Anthony A. Bonaldo, Myrna C. Capurro, Margareth Lara |
| dc.subject.en.pt_BR.fl_str_mv |
Plasmodium gallinaceum Plasmodium falciparum Circumsporozoite protein Sindbis virus Transgenic mosquitoes Aedes aegypti |
| topic |
Plasmodium gallinaceum Plasmodium falciparum Circumsporozoite protein Sindbis virus Transgenic mosquitoes Aedes aegypti Plasmodium falciparum Aedes aegypti Vírus Sindbis Plasmodium gallinaceum |
| dc.subject.decs.pt_BR.fl_str_mv |
Plasmodium falciparum Aedes aegypti Vírus Sindbis Plasmodium gallinaceum |
| description |
Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Laboratório de Mosquitos Genéticamente Modificados. São. Paulo, SP, Brasil. |
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2016 |
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2016-07-02T18:45:00Z 2016-07-07T12:13:20Z |
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2016-07-02T18:45:00Z 2016-07-07T12:13:20Z |
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2016 |
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info:eu-repo/semantics/article |
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KOJIN, Bianca B. et al. Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands. Malaria Journal, v.15:153, 11p, 2016. |
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https://www.arca.fiocruz.br/handle/icict/14673 |
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1475-2875 |
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10.1186/s12936-016-1207-8 |
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1475-2875 |
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KOJIN, Bianca B. et al. Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands. Malaria Journal, v.15:153, 11p, 2016. 1475-2875 10.1186/s12936-016-1207-8 |
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BioMed Central |
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BioMed Central |
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