A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples

Detalhes bibliográficos
Autor(a) principal: Gandasegui, Javier
Data de Publicação: 2018
Outros Autores: Fernández-Soto, Pedro, Muro, Antonio, Barbosa, Constança Simões, Melo, Fabio Lopes de, Loyo, Rodrigo, Gomes, Elainne Christine de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33046
Resumo: University of Salamanca. Faculty of Pharmacy. Infectious and Tropical Diseases Research Group (e-INTRO). Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS). Salamanca, Spain.
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spelling Gandasegui, JavierFernández-Soto, PedroMuro, AntonioBarbosa, Constança SimõesMelo, Fabio Lopes deLoyo, RodrigoGomes, Elainne Christine de Souza2019-05-13T15:16:28Z2019-05-13T15:16:28Z2018GANDASEGUI, Javier et al. A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples. PLoS Neglected Tropical Diseases, v. 12, n. 3, p. 1-16, Mar. 13, 2018.1935-2735https://www.arca.fiocruz.br/handle/icict/3304610.1371/journal.pntd.0006314engA field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Salamanca. Faculty of Pharmacy. Infectious and Tropical Diseases Research Group (e-INTRO). Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS). Salamanca, Spain.University of Salamanca. Faculty of Pharmacy. Infectious and Tropical Diseases Research Group (e-INTRO). Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS). Salamanca, Spain.University of Salamanca. Faculty of Pharmacy. Infectious and Tropical Diseases Research Group (e-INTRO). Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS). Salamanca, Spain.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Esquistossomose e Serviço de Referência. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Esquistossomose e Serviço de Referência. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Esquistossomose e Serviço de Referência. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Parasitologia. Laboratório de Esquistossomose e Serviço de Referência. Recife, PE, Brasil.In Brazil, schistosomiasis is a parasitic disease of public health relevance, mainly in poor areas where Schistosoma mansoni is the only human species encountered and Biomphalaria straminea is one of the intermediate host snails. A nested-PCR based on a specific mitochondrial S. mansoni minisatellite DNA region has been successfully developed and applied as a reference method in Brazil for S. mansoni detection, mainly in host snails for epidemiological studies. The amplification efficiency of LAMP is known to be higher than PCR. The present work aimed to assess the utility of our previously described SmMIT-LAMP assay for S. mansoni detection in human stool and snail samples in a low-transmission area of schistosomiasis in the municipality of Umbuzeiro, Paraíba State, Northeast Region of Brazil.BiomphalariaDNADisease VectorsFecesSchistosoma mansoniProtozoanAdolescenteAdultoIdoso, 80 anos ou maisAnimaisBiomphalaria / parasitologiaBrasilDNA, protozoário / isolamento e purificaçãoVetores de DoençaFezes / parasitologiaFêmeaMasculinoMeia idadeTécnicas de amplificação de ácidos nucleicosReação em Cadeia da PolimeraseSchistosoma mansoni / isolamento & purificaçãoEsquistossomose mansoni / epidemiologiaAnálise espacialAdulto joveminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
title A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
spellingShingle A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
Gandasegui, Javier
Biomphalaria
DNA
Disease Vectors
Feces
Schistosoma mansoni
Protozoan
Adolescente
Adulto
Idoso, 80 anos ou mais
Animais
Biomphalaria / parasitologia
Brasil
DNA, protozoário / isolamento e purificação
Vetores de Doença
Fezes / parasitologia
Fêmea
Masculino
Meia idade
Técnicas de amplificação de ácidos nucleicos
Reação em Cadeia da Polimerase
Schistosoma mansoni / isolamento & purificação
Esquistossomose mansoni / epidemiologia
Análise espacial
Adulto jovem
title_short A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
title_full A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
title_fullStr A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
title_full_unstemmed A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
title_sort A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples
author Gandasegui, Javier
author_facet Gandasegui, Javier
Fernández-Soto, Pedro
Muro, Antonio
Barbosa, Constança Simões
Melo, Fabio Lopes de
Loyo, Rodrigo
Gomes, Elainne Christine de Souza
author_role author
author2 Fernández-Soto, Pedro
Muro, Antonio
Barbosa, Constança Simões
Melo, Fabio Lopes de
Loyo, Rodrigo
Gomes, Elainne Christine de Souza
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gandasegui, Javier
Fernández-Soto, Pedro
Muro, Antonio
Barbosa, Constança Simões
Melo, Fabio Lopes de
Loyo, Rodrigo
Gomes, Elainne Christine de Souza
dc.subject.en.pt_BR.fl_str_mv Biomphalaria
DNA
Disease Vectors
Feces
Schistosoma mansoni
Protozoan
topic Biomphalaria
DNA
Disease Vectors
Feces
Schistosoma mansoni
Protozoan
Adolescente
Adulto
Idoso, 80 anos ou mais
Animais
Biomphalaria / parasitologia
Brasil
DNA, protozoário / isolamento e purificação
Vetores de Doença
Fezes / parasitologia
Fêmea
Masculino
Meia idade
Técnicas de amplificação de ácidos nucleicos
Reação em Cadeia da Polimerase
Schistosoma mansoni / isolamento & purificação
Esquistossomose mansoni / epidemiologia
Análise espacial
Adulto jovem
dc.subject.decs.pt_BR.fl_str_mv Adolescente
Adulto
Idoso, 80 anos ou mais
Animais
Biomphalaria / parasitologia
Brasil
DNA, protozoário / isolamento e purificação
Vetores de Doença
Fezes / parasitologia
Fêmea
Masculino
Meia idade
Técnicas de amplificação de ácidos nucleicos
Reação em Cadeia da Polimerase
Schistosoma mansoni / isolamento & purificação
Esquistossomose mansoni / epidemiologia
Análise espacial
Adulto jovem
description University of Salamanca. Faculty of Pharmacy. Infectious and Tropical Diseases Research Group (e-INTRO). Biomedical Research Institute of Salamanca-Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS). Salamanca, Spain.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-05-13T15:16:28Z
dc.date.available.fl_str_mv 2019-05-13T15:16:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GANDASEGUI, Javier et al. A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples. PLoS Neglected Tropical Diseases, v. 12, n. 3, p. 1-16, Mar. 13, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33046
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0006314
identifier_str_mv GANDASEGUI, Javier et al. A field survey using LAMP assay for detection of Schistosoma mansoni in a low-transmission area of schistosomiasis in Umbuzeiro, Brazil: Assessment in human and snail samples. PLoS Neglected Tropical Diseases, v. 12, n. 3, p. 1-16, Mar. 13, 2018.
1935-2735
10.1371/journal.pntd.0006314
url https://www.arca.fiocruz.br/handle/icict/33046
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33046/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33046/2/A%20field%20survey%20using%20LAMP%20assay%20for%20detection%20of%20Schistosoma%20mansoni%20in%20a%20low-transmission%20area%20of%20schistosomiasis%20in%20Um.pdf
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