Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins

Detalhes bibliográficos
Autor(a) principal: Souza, Ana Paula Almeida de
Data de Publicação: 2013
Outros Autores: Soto, Manuel, Costa, Jackson Mauricio Lopes, Oliveira, Viviane Sampaio Boaventura de, Oliveira, Camila Indiani de, Cristal, Juqueline Rocha, Barral Netto, Manoel, Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7323
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Souza, Ana Paula Almeida deSoto, ManuelCosta, Jackson Mauricio LopesOliveira, Viviane Sampaio Boaventura deOliveira, Camila Indiani deCristal, Juqueline RochaBarral Netto, ManoelBarral, Aldina Maria Prado2014-02-18T17:26:09Z2014-02-18T17:26:09Z2013SOUZA, A. P. et al. Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins. Plos One, v. 8, p. e66110, 2013.1932-620310.1371/journal.pone.0066110https://www.arca.fiocruz.br/handle/icict/7323engUniversity of São PauloTowards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autónoma de Madrid. Departamento de Biología Molecular. Centro de Biología Molecular Severo Ochoa. Madrid, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, BrasilBACKGROUND: Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure. METHODOLOGY/PRINCIPAL FINDINGS: Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis (TL) against different L. infantum-chagasi-derived recombinant proteins (rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11). Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas' disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA. CONCLUSION: Our results raise possibility of using rHSP70 for the serodiagnosis of TL.Leishmania/imunologiaLeishmaniose/sangueLeishmaniose/diagnósticoProteínas de Protozoários/uso diagnósticoProteínas Recombinantes/uso diagnósticoTestes Sorológicos/métodosEnsaio de Imunoadsorção EnzimáticaProteínas de Choque Térmico HSP70/uso diagnósticoProteínas de Choque Térmico HSP70/metabolismoHumanosLeishmania/genéticaOligonucleotídeos/genéticaCurva ROCEstatísticas não Paramétricasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSouza A P Towards a More....pdfSouza A P Towards a More....pdfapplication/pdf2199911https://www.arca.fiocruz.br/bitstream/icict/7323/1/Souza%20A%20P%20Towards%20a%20More....pdf8ed1ae72e2ffbdbee4e84caf43d409c5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7323/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSouza A P Towards a More....pdf.txtSouza A P Towards a More....pdf.txtExtracted texttext/plain40570https://www.arca.fiocruz.br/bitstream/icict/7323/5/Souza%20A%20P%20Towards%20a%20More....pdf.txtc3c6c2ea33ba5af24b602f1f863fee08MD55THUMBNAILSouza A P Towards a More....pdf.jpgSouza A P Towards a More....pdf.jpgGenerated Thumbnailimage/jpeg2410https://www.arca.fiocruz.br/bitstream/icict/7323/4/Souza%20A%20P%20Towards%20a%20More....pdf.jpgade948b7a3bd5757afec305d9e780277MD54icict/73232023-03-15 14:32:56.256oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
title Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
spellingShingle Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
Souza, Ana Paula Almeida de
Leishmania/imunologia
Leishmaniose/sangue
Leishmaniose/diagnóstico
Proteínas de Protozoários/uso diagnóstico
Proteínas Recombinantes/uso diagnóstico
Testes Sorológicos/métodos
Ensaio de Imunoadsorção Enzimática
Proteínas de Choque Térmico HSP70/uso diagnóstico
Proteínas de Choque Térmico HSP70/metabolismo
Humanos
Leishmania/genética
Oligonucleotídeos/genética
Curva ROC
Estatísticas não Paramétricas
title_short Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
title_full Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
title_fullStr Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
title_full_unstemmed Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
title_sort Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins
author Souza, Ana Paula Almeida de
author_facet Souza, Ana Paula Almeida de
Soto, Manuel
Costa, Jackson Mauricio Lopes
Oliveira, Viviane Sampaio Boaventura de
Oliveira, Camila Indiani de
Cristal, Juqueline Rocha
Barral Netto, Manoel
Barral, Aldina Maria Prado
author_role author
author2 Soto, Manuel
Costa, Jackson Mauricio Lopes
Oliveira, Viviane Sampaio Boaventura de
Oliveira, Camila Indiani de
Cristal, Juqueline Rocha
Barral Netto, Manoel
Barral, Aldina Maria Prado
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Ana Paula Almeida de
Soto, Manuel
Costa, Jackson Mauricio Lopes
Oliveira, Viviane Sampaio Boaventura de
Oliveira, Camila Indiani de
Cristal, Juqueline Rocha
Barral Netto, Manoel
Barral, Aldina Maria Prado
dc.subject.decs.pt_BR.fl_str_mv Leishmania/imunologia
Leishmaniose/sangue
Leishmaniose/diagnóstico
Proteínas de Protozoários/uso diagnóstico
Proteínas Recombinantes/uso diagnóstico
Testes Sorológicos/métodos
Ensaio de Imunoadsorção Enzimática
Proteínas de Choque Térmico HSP70/uso diagnóstico
Proteínas de Choque Térmico HSP70/metabolismo
Humanos
Leishmania/genética
Oligonucleotídeos/genética
Curva ROC
Estatísticas não Paramétricas
topic Leishmania/imunologia
Leishmaniose/sangue
Leishmaniose/diagnóstico
Proteínas de Protozoários/uso diagnóstico
Proteínas Recombinantes/uso diagnóstico
Testes Sorológicos/métodos
Ensaio de Imunoadsorção Enzimática
Proteínas de Choque Térmico HSP70/uso diagnóstico
Proteínas de Choque Térmico HSP70/metabolismo
Humanos
Leishmania/genética
Oligonucleotídeos/genética
Curva ROC
Estatísticas não Paramétricas
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-02-18T17:26:09Z
dc.date.available.fl_str_mv 2014-02-18T17:26:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, A. P. et al. Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins. Plos One, v. 8, p. e66110, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7323
dc.identifier.issn.none.fl_str_mv 1932-6203
10.1371/journal.pone.0066110
identifier_str_mv SOUZA, A. P. et al. Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins. Plos One, v. 8, p. e66110, 2013.
1932-6203
10.1371/journal.pone.0066110
url https://www.arca.fiocruz.br/handle/icict/7323
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv University of São Paulo
publisher.none.fl_str_mv University of São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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