Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection

Detalhes bibliográficos
Autor(a) principal: Nosanchuk, J. D.
Data de Publicação: 2002
Outros Autores: Gomez, B. L., Youngchim, S., Diez, S., Aisen, P., Oliveira, R. M. Z., Restrepo, A., Casadevall, A., Hamilton, A. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33095
Resumo: 2020-05-15
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spelling Nosanchuk, J. D.Gomez, B. L.Youngchim, S.Diez, S.Aisen, P.Oliveira, R. M. Z.Restrepo, A.Casadevall, A.Hamilton, A. J.2019-05-15T22:08:20Z2019-05-15T22:08:20Z2002NOSANCHUK, Joshua D. et al. Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection, v. 70, n. 9, p. 5124-5131, Sept. 2002.0019-9567https://www.arca.fiocruz.br/handle/icict/3309510.1128/IAI.70.9.5124–5131.20021098-5522engAmerican Society for MicrobiologyHistoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-05-15Albert Einstein College of Medicine. Department of Medicine. New York, NY, USA.Guy’s, Kings, and St. Thomas Medical Schools.Guy’s Hospital. St. Johns Institute of Dermatology. Dermatology Department. London, UK / Corporación para Investigaciones Biológicas. Medellín, Colombia.Guy’s, Kings, and St. Thomas Medical Schools.Guy’s Hospital. St. Johns. Institute of Dermatology. Dermatology Department. London, UK / Chiang-Mai Medical School. Microbiology Department. Chiang-Mai, Thailand.Guy’s, Kings, and St. Thomas Medical Schools.Guy’s Hospital. St. Johns Institute of Dermatology. Dermatology Department. London, UK / Corporación para Investigaciones Biológicas. Medellín, Colombia.Albert Einstein College of Medicine. Department of Physiology and Biophysics. New York, NY, USA.Fundação Oswaldo Cruz. Centro de Pesquisas Hospital Evandro Chagas. Rio de Janeiro, RJ, Brasil.Corporación para Investigaciones Biológicas. Medellín, Colombia.Albert Einstein College of Medicine. Department of Medicine. New York, NY, USA / Albert Einstein College of Medicine. Department of Microbiology and Immunology. New York, NY, USA.Guy’s, Kings, and St. Thomas Medical Schools.Guy’s Hospital. St. Johns. Institute of Dermatology. Dermatology Department. London, UK.Melanin is made by several important pathogenic fungi and has been implicated in the pathogenesis of a number of fungal infections. This study investigated whether the thermally dimorphic fungal pathogen Histoplasma capsulatum var. capsulatum produced melanin or melanin-like compounds in vitro and during infection. Growth of H. capsulatum mycelia in chemically defined minimal medium produced pigmented conidia. Growth of H. capsulatum yeast in chemically defined minimal medium with L-3,4-dihydroxyphenylalanine (DOPA) or (-)-epinephrine produced pigmented cells. Treatment of the pigmented cells with proteolytic enzymes, denaturant, and hot concentrated acid yielded dark particles that were similar in size and shape to their respective propagules. Melanin-binding monoclonal antibodies (MAb) labeled pigmented conidia, yeast, and the isolated particles as determined by immunofluorescence microscopy. Electron spin resonance spectroscopy revealed that pigmented yeast cells and particles derived from pigmented cells were stable free radicals consistent with their identification as melanins. Tissues from mice infected with H. capsulatum and from biopsy specimens from a patient with histoplasmosis contained fungal cells that were labeled by melaninbinding MAb. Digestion of infected mouse tissues yielded dark particles that reacted with the melanin-binding MAb and were similar in appearance to H. capsulatum yeast cells. Additionally, sera from infected mice contained antibodies that bound melanin particles. Phenoloxidase activity capable of synthesizing melanin from L-DOPA was detected in cytoplasmic yeast cell extracts. These findings indicate that H. capsulatum conidia and yeast can produce melanin or melanin-like compounds in vitro and that yeast cells can synthesize pigment in vivo. Since melanin is an important virulence factor in other pathogenic fungi, this pigment may have a similar role to play in the pathogenesis of histoplasmosis.Histoplasma capsulatumPigments in vitroMammalian infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33095/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Nosanchuk_Joshua_etal_INI_2002.pdfve_Nosanchuk_Joshua_etal_INI_2002.pdfapplication/pdf964037https://www.arca.fiocruz.br/bitstream/icict/33095/2/ve_Nosanchuk_Joshua_etal_INI_2002.pdf524cae21d14af9f6fa30b28e855886efMD52TEXTve_Nosanchuk_Joshua_etal_INI_2002.pdf.txtve_Nosanchuk_Joshua_etal_INI_2002.pdf.txtExtracted texttext/plain44924https://www.arca.fiocruz.br/bitstream/icict/33095/3/ve_Nosanchuk_Joshua_etal_INI_2002.pdf.txt25d9ef3a881b089fd46c04900880a696MD53icict/330952021-03-24 16:32:31.616oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:31Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
title Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
spellingShingle Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
Nosanchuk, J. D.
Histoplasma capsulatum
Pigments in vitro
Mammalian infection
title_short Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
title_full Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
title_fullStr Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
title_full_unstemmed Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
title_sort Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection
author Nosanchuk, J. D.
author_facet Nosanchuk, J. D.
Gomez, B. L.
Youngchim, S.
Diez, S.
Aisen, P.
Oliveira, R. M. Z.
Restrepo, A.
Casadevall, A.
Hamilton, A. J.
author_role author
author2 Gomez, B. L.
Youngchim, S.
Diez, S.
Aisen, P.
Oliveira, R. M. Z.
Restrepo, A.
Casadevall, A.
Hamilton, A. J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nosanchuk, J. D.
Gomez, B. L.
Youngchim, S.
Diez, S.
Aisen, P.
Oliveira, R. M. Z.
Restrepo, A.
Casadevall, A.
Hamilton, A. J.
dc.subject.en.pt_BR.fl_str_mv Histoplasma capsulatum
Pigments in vitro
Mammalian infection
topic Histoplasma capsulatum
Pigments in vitro
Mammalian infection
description 2020-05-15
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2019-05-15T22:08:20Z
dc.date.available.fl_str_mv 2019-05-15T22:08:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NOSANCHUK, Joshua D. et al. Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection, v. 70, n. 9, p. 5124-5131, Sept. 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33095
dc.identifier.issn.pt_BR.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.70.9.5124–5131.2002
dc.identifier.eissn.none.fl_str_mv 1098-5522
identifier_str_mv NOSANCHUK, Joshua D. et al. Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection, v. 70, n. 9, p. 5124-5131, Sept. 2002.
0019-9567
10.1128/IAI.70.9.5124–5131.2002
1098-5522
url https://www.arca.fiocruz.br/handle/icict/33095
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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