Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8154 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil |
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Solcà, Manuela da SilvaBastos, Leila AndradeGuedes, Carlos Eduardo SampaioBordoni, MarceloBorja, Lairton SouzaLarangeira, Daniela FariasTuy, Pétala Gardênia da Silva EstrelaAmorim, Leila Denise Alves FerreiraNascimento, Eliane GomesOliveira, Geraldo Gileno de SádosSantos, Washington Luis ConradoFraga, Deborah Bittencourt MothéVeras, Patrícia Sampaio Tavares2014-08-08T17:03:03Z2014-08-08T17:03:03Z2014SOLCA, M. da S. et al. Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis. PloS One, v. 9, n. 7, p. e103635, 2014.1932-6203https://www.arca.fiocruz.br/handle/icict/815410.1371/journal.pone.0103635engPublic Library of ScienceLeishmania infantumLeishmania visceralAnimaisCãesDiagnósticoPCREvaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, BrasilCentro de Referência em Doenças Endêmicas Pirajá da Silva - PIEJ. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT - DT. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT - DT. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT - DT. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais - INCT - DT. Salvador, BA, BrasilHost tissues affected by Leishmania infantum have differing degrees of parasitism. Previously, the use of different biological tissues to detect L. infantum DNA in dogs has provided variable results. The present study was conducted to evaluate the accuracy of molecular diagnostic testing (qPCR) in dogs from an endemic area for canine visceral leishmaniasis (CVL) by determining which tissue type provided the highest rate of parasite DNA detection. Fifty-one symptomatic dogs were tested for CVL using serological, parasitological and molecular methods. Latent class analysis (LCA) was performed for accuracy evaluation of these methods. qPCR detected parasite DNA in 100% of these animals from at least one of the following tissues: splenic and bone marrow aspirates, lymph node and skin fragments, blood and conjunctival swabs. Using latent variable as gold standard, the qPCR achieved a sensitivity of 95.8% (CI 90.4–100) in splenic aspirate; 79.2% (CI 68–90.3) in lymph nodes; 77.3% (CI 64.5–90.1) in skin; 75% (CI 63.1–86.9) in blood; 50% (CI 30–70) in bone marrow; 37.5% (CI 24.2– 50.8) in left-eye; and 29.2% (CI 16.7–41.6) in right-eye conjunctival swabs. The accuracy of qPCR using splenic aspirates was further evaluated in a random larger sample (n = 800), collected from dogs during a prevalence study. The specificity achieved by qPCR was 76.7% (CI 73.7–79.6) for splenic aspirates obtained from the greater sample. The sensitivity accomplished by this technique was 95% (CI 93.5–96.5) that was higher than those obtained for the other diagnostic tests and was similar to that observed in the smaller sampling study. This confirms that the splenic aspirate is the most effective type of tissue for detecting L. infantum infection. Additionally, we demonstrated that LCA could be used to generate a suitable gold standard for comparative CVL testing.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSolca M S Evaluating the accuracy....pdfSolca M S Evaluating the accuracy....pdfapplication/pdf426203https://www.arca.fiocruz.br/bitstream/icict/8154/1/Solca%20M%20S%20Evaluating%20the%20accuracy....pdffc2399c94a6f2a727a6542f25aa72997MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8154/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSolca M S Evaluating the accuracy....pdf.txtSolca M S Evaluating the accuracy....pdf.txtExtracted texttext/plain65888https://www.arca.fiocruz.br/bitstream/icict/8154/3/Solca%20M%20S%20Evaluating%20the%20accuracy....pdf.txt17d787a46c465dd5fc2c327730df5a27MD53icict/81542023-03-15 14:33:00.17oai:www.arca.fiocruz.br:icict/8154TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis |
title |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis |
spellingShingle |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis Solcà, Manuela da Silva Leishmania infantum Leishmania visceral Animais Cães Diagnóstico PCR |
title_short |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis |
title_full |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis |
title_fullStr |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis |
title_full_unstemmed |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis |
title_sort |
Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis |
author |
Solcà, Manuela da Silva |
author_facet |
Solcà, Manuela da Silva Bastos, Leila Andrade Guedes, Carlos Eduardo Sampaio Bordoni, Marcelo Borja, Lairton Souza Larangeira, Daniela Farias Tuy, Pétala Gardênia da Silva Estrela Amorim, Leila Denise Alves Ferreira Nascimento, Eliane Gomes Oliveira, Geraldo Gileno de Sá dosSantos, Washington Luis Conrado Fraga, Deborah Bittencourt Mothé Veras, Patrícia Sampaio Tavares |
author_role |
author |
author2 |
Bastos, Leila Andrade Guedes, Carlos Eduardo Sampaio Bordoni, Marcelo Borja, Lairton Souza Larangeira, Daniela Farias Tuy, Pétala Gardênia da Silva Estrela Amorim, Leila Denise Alves Ferreira Nascimento, Eliane Gomes Oliveira, Geraldo Gileno de Sá dosSantos, Washington Luis Conrado Fraga, Deborah Bittencourt Mothé Veras, Patrícia Sampaio Tavares |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Solcà, Manuela da Silva Bastos, Leila Andrade Guedes, Carlos Eduardo Sampaio Bordoni, Marcelo Borja, Lairton Souza Larangeira, Daniela Farias Tuy, Pétala Gardênia da Silva Estrela Amorim, Leila Denise Alves Ferreira Nascimento, Eliane Gomes Oliveira, Geraldo Gileno de Sá dosSantos, Washington Luis Conrado Fraga, Deborah Bittencourt Mothé Veras, Patrícia Sampaio Tavares |
dc.subject.other.pt_BR.fl_str_mv |
Leishmania infantum Leishmania visceral Animais Cães Diagnóstico PCR |
topic |
Leishmania infantum Leishmania visceral Animais Cães Diagnóstico PCR |
description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-08-08T17:03:03Z |
dc.date.available.fl_str_mv |
2014-08-08T17:03:03Z |
dc.date.issued.fl_str_mv |
2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOLCA, M. da S. et al. Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis. PloS One, v. 9, n. 7, p. e103635, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8154 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0103635 |
identifier_str_mv |
SOLCA, M. da S. et al. Evaluating the accuracy of molecular diagnostic testing for Canine Visceral Leishmaniasis using latent class analysis. PloS One, v. 9, n. 7, p. e103635, 2014. 1932-6203 10.1371/journal.pone.0103635 |
url |
https://www.arca.fiocruz.br/handle/icict/8154 |
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eng |
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eng |
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Public Library of Science |
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Public Library of Science |
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