Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40935 |
Resumo: | Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. |
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Oliveira, Iara dos Santos da SilvaColares, Aracélio VianaCardoso, Flávia de OliveiraTellis, Carla Junqueira MoragasChagas, Maria do Socorro dos SantosBehrens, Maria DutraCalabrese, Kátia da SilvaSouza, Fernando AlmeidaSilva, Ana Lúcia Abreu2020-04-23T15:15:30Z2020-04-23T15:15:30Z2019OLIVEIRA, Iara dos Santos da Silva et al. Vernonia polysphaera Baker: Antiinflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells. PLoS ONE, v. 14, n. 12, p. 1-19, 12 Dec. 2019.1932-6203https://www.arca.fiocruz.br/handle/icict/4093510.1371/journal.pone.0225275engPublic Library of ScienceVernonia polysphaera BakerAtividade anti-inflamatóriaIn vivoCélulas RAW 264.7Efeito inibitórioVernonia polysphaera BakerAntiinflammatory activityIn vivoRAW 264.7 cellsInhibitory effectVernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil.Centro Universitário Dr. Leão Sampaio. Juazeiro do Norte, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Departamento de Produtos Naturais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Departamento de Produtos Naturais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Departamento de Produtos Naturais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhão. Pós-Graduação em Ciência Animal. São Luis, MA, Brasil.Universidade Estadual do Maranhão. Pós-Graduação em Ciência Animal. São Luis, MA, Brasil.Species of the Vernonia genius are widely distributed across the world. In traditional communities, they are commonly used in popular medicine for the treatment of inflammatory diseases. The objective of the present study was to evaluate the anti-inflammatory activity of Vernonia polysphaera Baker hydroalcoholic extract. A λ-carrageenan-induced paw edema and peritonitis model was established in BALB/c mice. The in vitro activity of the extract was measured on LPS-stimulated RAW 264.7 cells. There was no toxic effect on mice or on the cells treated with the extract. Animals treated with V. polysphaera extract demonstrated inhibition of paw edema in comparison with the untreated animals at all the analyzed doses. In peritonitis, treatment with the extract at a dose of 500 mg/kg resulted in a lower total leukocyte count in the peritoneal fluid and blood and lower levels of IL-1β, IL-6, TNF-α and PGE-2 than the control group. Cells treated with 50 and 100 μg/mL of the extract exhibited lower levels of nitrite and pro-inflammatory cytokine production and lower COX-2, NF-κB expression. The V. polysphaera extract demonstrated an anti-inflammatory effect, interfering with cell migration, reducing pro-inflammatory cytokine levels and COX-2 expression and consequent interference with PGE-2, as well as inhibiting NF-κB transcription.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40935/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALKatiaCalabrese_FOCardoso_etal_IOC_2019.pdfKatiaCalabrese_FOCardoso_etal_IOC_2019.pdfapplication/pdf2998986https://www.arca.fiocruz.br/bitstream/icict/40935/2/KatiaCalabrese_FOCardoso_etal_IOC_2019.pdf5349c4da6f0435669ce406d7a1165fa6MD52TEXTKatiaCalabrese_FOCardoso_etal_IOC_2019.pdf.txtKatiaCalabrese_FOCardoso_etal_IOC_2019.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells |
| title |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells |
| spellingShingle |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells Oliveira, Iara dos Santos da Silva Vernonia polysphaera Baker Atividade anti-inflamatória In vivo Células RAW 264.7 Efeito inibitório Vernonia polysphaera Baker Antiinflammatory activity In vivo RAW 264.7 cells Inhibitory effect |
| title_short |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells |
| title_full |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells |
| title_fullStr |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells |
| title_full_unstemmed |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells |
| title_sort |
Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells |
| author |
Oliveira, Iara dos Santos da Silva |
| author_facet |
Oliveira, Iara dos Santos da Silva Colares, Aracélio Viana Cardoso, Flávia de Oliveira Tellis, Carla Junqueira Moragas Chagas, Maria do Socorro dos Santos Behrens, Maria Dutra Calabrese, Kátia da Silva Souza, Fernando Almeida Silva, Ana Lúcia Abreu |
| author_role |
author |
| author2 |
Colares, Aracélio Viana Cardoso, Flávia de Oliveira Tellis, Carla Junqueira Moragas Chagas, Maria do Socorro dos Santos Behrens, Maria Dutra Calabrese, Kátia da Silva Souza, Fernando Almeida Silva, Ana Lúcia Abreu |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Iara dos Santos da Silva Colares, Aracélio Viana Cardoso, Flávia de Oliveira Tellis, Carla Junqueira Moragas Chagas, Maria do Socorro dos Santos Behrens, Maria Dutra Calabrese, Kátia da Silva Souza, Fernando Almeida Silva, Ana Lúcia Abreu |
| dc.subject.other.pt_BR.fl_str_mv |
Vernonia polysphaera Baker Atividade anti-inflamatória In vivo Células RAW 264.7 Efeito inibitório |
| topic |
Vernonia polysphaera Baker Atividade anti-inflamatória In vivo Células RAW 264.7 Efeito inibitório Vernonia polysphaera Baker Antiinflammatory activity In vivo RAW 264.7 cells Inhibitory effect |
| dc.subject.en.pt_BR.fl_str_mv |
Vernonia polysphaera Baker Antiinflammatory activity In vivo RAW 264.7 cells Inhibitory effect |
| description |
Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. |
| publishDate |
2019 |
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2019 |
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2020-04-23T15:15:30Z |
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2020-04-23T15:15:30Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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OLIVEIRA, Iara dos Santos da Silva et al. Vernonia polysphaera Baker: Antiinflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells. PLoS ONE, v. 14, n. 12, p. 1-19, 12 Dec. 2019. |
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https://www.arca.fiocruz.br/handle/icict/40935 |
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1932-6203 |
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10.1371/journal.pone.0225275 |
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OLIVEIRA, Iara dos Santos da Silva et al. Vernonia polysphaera Baker: Antiinflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells. PLoS ONE, v. 14, n. 12, p. 1-19, 12 Dec. 2019. 1932-6203 10.1371/journal.pone.0225275 |
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