Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46991 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas à Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. |
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Costa, Alexandre Dias TavaresJacomasso, ThiagoMattos, Elaine C.Farias, Aline B.Rampazzo, Rita C.P.Pinto, Rebeka S.Tassi, WalleydMarciano, Maria Aparecida M.Pereira-Chioccola, Vera LuciaMurphy, Helen R.Silva, Alexandre J. daKrieger, Marco Aurélio2021-04-30T20:08:09Z2021-04-30T20:08:09Z2021COSTA, Alexandre D. T. et al. Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices. Food and Waterborne Parasitology, n. 22, p. 1–12, 2021.2405-6766https://www.arca.fiocruz.br/handle/icict/4699110.1016/j.fawpar.2021.e00111porElsevierCyclospora cayetanensisTrypanosoma cruziqPCRFoodborne DiseasesMolecular DiagnosticsProtozoan InfectionsQuantitative Real-Time Polymerase Chain ReactionEnfermedades Transmitidas por los AlimentosPatología MolecularInfecciones por ProtozoosReacción en Cadena en Tiempo Real de la PolimerasaMaladies d'origine alimentaireAnatomopathologie moléculaireProtozoosesRéaction de polymérisation en chaine quantitative en temps réelCyclosporaDoenças Transmitidas por AlimentosPatologia MolecularInfecções por ProtozoáriosReação em Cadeia da Polimerase em Tempo RealReady-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matricesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas à Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto Adolfo Lutz. Santo André, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.U.S. Food & Drug Administration Center for Food Safety and Applied Nutrition (CFSAN). Office of Applied Research and Safety Assessment. Laurel, MD, USA.U.S. Food & Drug Administration Center for Food Safety and Applied Nutrition (CFSAN). Office of Applied Research and Safety Assessment. Laurel, MD, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas à Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Foodborne outbreaks caused by parasites have long been a public health issue. Among the available contamination detection methods, qPCR is one of the most sensitive and specific. However, it can be cumbersome and error-prone, if used by unexperienced users. Moreover, qPCR reagents usually require freezer temperatures for transportation and storage. We present a gelified reaction format that allows the reagents to be stored at 2–8 °C for up to 90 days without losing performance. The gelification process eliminates most operator mistakes during reaction setup, and renders the qPCR plates ready-to-use. The new reaction makeup was evaluated using artificially contaminated samples of distinct food matrices for sensitivity, specificity, repeatability, reproducibility, and stability. Samples consisted of cilantro leaves and raspberry fruits spiked with Cyclospora cayetanensis oocysts, as well as açai pulp and sugarcane juice tainted with Trypanosoma cruzi trypomastigotes. No significant difference between the gelified and the non-gelified qPCR was found. Our results suggest that gelifying the assay may help to achieve more reproducible qPCR data across laboratories, thus supporting surveillance actions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/46991/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALFoodWaterborneok.pdfFoodWaterborneok.pdfapplication/pdf1522007https://www.arca.fiocruz.br/bitstream/icict/46991/2/FoodWaterborneok.pdf23a56bb15ef3aca4a6ad843dc4e9ceefMD52TEXTFoodWaterborneok.pdf.txtFoodWaterborneok.pdf.txtExtracted texttext/plain59129https://www.arca.fiocruz.br/bitstream/icict/46991/3/FoodWaterborneok.pdf.txt20c02557d3536aea6d5d45379df3a077MD53icict/469912021-05-01 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| dc.title.pt_BR.fl_str_mv |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices |
| title |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices |
| spellingShingle |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices Costa, Alexandre Dias Tavares Cyclospora cayetanensis Trypanosoma cruzi qPCR Foodborne Diseases Molecular Diagnostics Protozoan Infections Quantitative Real-Time Polymerase Chain Reaction Enfermedades Transmitidas por los Alimentos Patología Molecular Infecciones por Protozoos Reacción en Cadena en Tiempo Real de la Polimerasa Maladies d'origine alimentaire Anatomopathologie moléculaire Protozooses Réaction de polymérisation en chaine quantitative en temps réel Cyclospora Doenças Transmitidas por Alimentos Patologia Molecular Infecções por Protozoários Reação em Cadeia da Polimerase em Tempo Real |
| title_short |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices |
| title_full |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices |
| title_fullStr |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices |
| title_full_unstemmed |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices |
| title_sort |
Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices |
| author |
Costa, Alexandre Dias Tavares |
| author_facet |
Costa, Alexandre Dias Tavares Jacomasso, Thiago Mattos, Elaine C. Farias, Aline B. Rampazzo, Rita C.P. Pinto, Rebeka S. Tassi, Walleyd Marciano, Maria Aparecida M. Pereira-Chioccola, Vera Lucia Murphy, Helen R. Silva, Alexandre J. da Krieger, Marco Aurélio |
| author_role |
author |
| author2 |
Jacomasso, Thiago Mattos, Elaine C. Farias, Aline B. Rampazzo, Rita C.P. Pinto, Rebeka S. Tassi, Walleyd Marciano, Maria Aparecida M. Pereira-Chioccola, Vera Lucia Murphy, Helen R. Silva, Alexandre J. da Krieger, Marco Aurélio |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Costa, Alexandre Dias Tavares Jacomasso, Thiago Mattos, Elaine C. Farias, Aline B. Rampazzo, Rita C.P. Pinto, Rebeka S. Tassi, Walleyd Marciano, Maria Aparecida M. Pereira-Chioccola, Vera Lucia Murphy, Helen R. Silva, Alexandre J. da Krieger, Marco Aurélio |
| dc.subject.other.pt_BR.fl_str_mv |
Cyclospora cayetanensis Trypanosoma cruzi qPCR |
| topic |
Cyclospora cayetanensis Trypanosoma cruzi qPCR Foodborne Diseases Molecular Diagnostics Protozoan Infections Quantitative Real-Time Polymerase Chain Reaction Enfermedades Transmitidas por los Alimentos Patología Molecular Infecciones por Protozoos Reacción en Cadena en Tiempo Real de la Polimerasa Maladies d'origine alimentaire Anatomopathologie moléculaire Protozooses Réaction de polymérisation en chaine quantitative en temps réel Cyclospora Doenças Transmitidas por Alimentos Patologia Molecular Infecções por Protozoários Reação em Cadeia da Polimerase em Tempo Real |
| dc.subject.en.pt_BR.fl_str_mv |
Foodborne Diseases Molecular Diagnostics Protozoan Infections Quantitative Real-Time Polymerase Chain Reaction |
| dc.subject.es.pt_BR.fl_str_mv |
Enfermedades Transmitidas por los Alimentos Patología Molecular Infecciones por Protozoos Reacción en Cadena en Tiempo Real de la Polimerasa |
| dc.subject.fr.pt_BR.fl_str_mv |
Maladies d'origine alimentaire Anatomopathologie moléculaire Protozooses Réaction de polymérisation en chaine quantitative en temps réel |
| dc.subject.decs.pt_BR.fl_str_mv |
Cyclospora Doenças Transmitidas por Alimentos Patologia Molecular Infecções por Protozoários Reação em Cadeia da Polimerase em Tempo Real |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas à Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. |
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2021 |
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2021-04-30T20:08:09Z |
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2021-04-30T20:08:09Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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COSTA, Alexandre D. T. et al. Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices. Food and Waterborne Parasitology, n. 22, p. 1–12, 2021. |
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https://www.arca.fiocruz.br/handle/icict/46991 |
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2405-6766 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.fawpar.2021.e00111 |
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COSTA, Alexandre D. T. et al. Ready-to-use qPCR for detection of Cyclospora cayetanensis or Trypanosoma cruzi in food matrices. Food and Waterborne Parasitology, n. 22, p. 1–12, 2021. 2405-6766 10.1016/j.fawpar.2021.e00111 |
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Elsevier |
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Elsevier |
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