Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays
| Autor(a) principal: | |
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| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30688 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil |
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Mendes, Tiago Antônio de OliveiraCunha, João Luís ReisLourdes, Rodrigo de AlmeidaLuiz, Gabriela Flávia RodriguesLemos, Lucas DhomSantos, Ana Rita Rocha dosCâmara, Antônia Cláudia Jácome daGalvão, Lúcia Maria da CunhaBern, CarynGilman, Robert H.Fujiwara, Ricardo ToshioGazzinelli, Ricardo TostesBartholomeu, Daniella Castanheira2018-12-19T11:31:26Z2018-12-19T11:31:26Z2013MENDES, Tiago Antônio de Oliveira et al. Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays. PLoS Negl Trop Dis., v. 7, n. 10, e2524, 20131935-2735https://www.arca.fiocruz.br/handle/icict/3068810.1371/journal.pntd.0002524. eCollection 2013engPublic Library of ScienceDoença de ChagasChagas diseaseIdentification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arraysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Natal, RN, BrasilUniversidade Federal do Rio Grande do Norte. Centro de Ciências da Saúde. Natal, RN, BrasilUniversity of California. San Francisco, CA, United States of AmericaUniversidad Cayetano Heredia. Lima, Peru/Johns Hopkins University. Baltimore, Maryland, United States of AmericaUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil/Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of AmericUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilBACKGROUND: The factors influencing variation in the clinical forms of Chagas disease have not been elucidated; however, it is likely that the genetics of both the host and the parasite are involved. Several studies have attempted to correlate the T. cruzi strains involved in infection with the clinical forms of the disease by using hemoculture and/or PCR-based genotyping of parasites from infected human tissues. However, both techniques have limitations that hamper the analysis of large numbers of samples. The goal of this work was to identify conserved and polymorphic linear B-cell epitopes of T. cruzi that could be used for serodiagnosis and serotyping of Chagas disease using ELISA. METHODOLOGY: By performing B-cell epitope prediction on proteins derived from pair of alleles of the hybrid CL Brener genome, we have identified conserved and polymorphic epitopes in the two CL Brener haplotypes. The rationale underlying this strategy is that, because CL Brener is a recent hybrid between the TcII and TcIII DTUs (discrete typing units), it is likely that polymorphic epitopes in pairs of alleles could also be polymorphic in the parental genotypes. We excluded sequences that are also present in the Leishmania major, L. infantum, L. braziliensis and T. brucei genomes to minimize the chance of cross-reactivity. A peptide array containing 150 peptides was covalently linked to a cellulose membrane, and the reactivity of the peptides was tested using sera from C57BL/6 mice chronically infected with the Colombiana (TcI) and CL Brener (TcVI) clones and Y (TcII) strain. FINDINGS AND CONCLUSIONS: A total of 36 peptides were considered reactive, and the cross-reactivity among the strains is in agreement with the evolutionary origin of the different T. cruzi DTUs. Four peptides were tested against a panel of chagasic patients using ELISA. A conserved peptide showed 95.8% sensitivity, 88.5% specificity, and 92.7% accuracy for the identification of T. cruzi in patients infected with different strains of the parasite. Therefore, this peptide, in association with other T. cruzi antigens, may improve Chagas disease serodiagnosis. Together, three polymorphic epitopes were able to discriminate between the three parasite strains used in this study and are thus potential targets for Chagas disease serotyping.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30688/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALIdentification of Strain-Specific B-cell Epitopes.pdfIdentification of Strain-Specific B-cell Epitopes.pdfapplication/pdf1385855https://www.arca.fiocruz.br/bitstream/icict/30688/2/Identification%20of%20Strain-Specific%20B-cell%20Epitopes.pdf56137343c2ee76c184b711dbbd2297d9MD52TEXTIdentification of Strain-Specific B-cell Epitopes.pdf.txtIdentification of Strain-Specific B-cell Epitopes.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
| title |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
| spellingShingle |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays Mendes, Tiago Antônio de Oliveira Doença de Chagas Chagas disease |
| title_short |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
| title_full |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
| title_fullStr |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
| title_full_unstemmed |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
| title_sort |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
| author |
Mendes, Tiago Antônio de Oliveira |
| author_facet |
Mendes, Tiago Antônio de Oliveira Cunha, João Luís Reis Lourdes, Rodrigo de Almeida Luiz, Gabriela Flávia Rodrigues Lemos, Lucas Dhom Santos, Ana Rita Rocha dos Câmara, Antônia Cláudia Jácome da Galvão, Lúcia Maria da Cunha Bern, Caryn Gilman, Robert H. Fujiwara, Ricardo Toshio Gazzinelli, Ricardo Tostes Bartholomeu, Daniella Castanheira |
| author_role |
author |
| author2 |
Cunha, João Luís Reis Lourdes, Rodrigo de Almeida Luiz, Gabriela Flávia Rodrigues Lemos, Lucas Dhom Santos, Ana Rita Rocha dos Câmara, Antônia Cláudia Jácome da Galvão, Lúcia Maria da Cunha Bern, Caryn Gilman, Robert H. Fujiwara, Ricardo Toshio Gazzinelli, Ricardo Tostes Bartholomeu, Daniella Castanheira |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mendes, Tiago Antônio de Oliveira Cunha, João Luís Reis Lourdes, Rodrigo de Almeida Luiz, Gabriela Flávia Rodrigues Lemos, Lucas Dhom Santos, Ana Rita Rocha dos Câmara, Antônia Cláudia Jácome da Galvão, Lúcia Maria da Cunha Bern, Caryn Gilman, Robert H. Fujiwara, Ricardo Toshio Gazzinelli, Ricardo Tostes Bartholomeu, Daniella Castanheira |
| dc.subject.other.pt_BR.fl_str_mv |
Doença de Chagas |
| topic |
Doença de Chagas Chagas disease |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas disease |
| description |
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil |
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2013 |
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2013 |
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2018-12-19T11:31:26Z |
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2018-12-19T11:31:26Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MENDES, Tiago Antônio de Oliveira et al. Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays. PLoS Negl Trop Dis., v. 7, n. 10, e2524, 2013 |
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https://www.arca.fiocruz.br/handle/icict/30688 |
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1935-2735 |
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10.1371/journal.pntd.0002524. eCollection 2013 |
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MENDES, Tiago Antônio de Oliveira et al. Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays. PLoS Negl Trop Dis., v. 7, n. 10, e2524, 2013 1935-2735 10.1371/journal.pntd.0002524. eCollection 2013 |
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Public Library of Science |
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Public Library of Science |
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