Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin

Detalhes bibliográficos
Autor(a) principal: Nascimento, Dilzamar V.
Data de Publicação: 2017
Outros Autores: Dellagostin, Odir A., Matos, Denise C. S., McIntosh, Douglas, Hirata Jr., Raphael, Pereira, Geraldo M. B., Guaraldi, Ana Luiza de Mattos, Armôa, Geraldo R. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24909
Resumo: Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
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spelling Nascimento, Dilzamar V.Dellagostin, Odir A.Matos, Denise C. S.McIntosh, DouglasHirata Jr., RaphaelPereira, Geraldo M. B.Guaraldi, Ana Luiza de MattosArmôa, Geraldo R. G.2018-02-22T12:12:49Z2018-02-22T12:12:49Z2017NASCIMENTO, Dilzamar V. et al. Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin. American Journal of Molecular Biology, v.7, p.176-189, 2017.2161-6620https://www.arca.fiocruz.br/handle/icict/249092161-6663engScientific Research Publishing IncToxina DiftéricaCorynebacterium diphtheriaeDifteriaMycobacterium bovis BCGRecombinant BCGDiphtheria Toxin dtb GenePark Williams 8 (PW8)Corynebacterium diphtheriaerDTBPW8pUS977 VectorMycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade Federal de Pelotas. Campus Universitário. Núcleo de Biotecnologia. Pelotas, RS, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ. Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilDiphtheria is a fulminant bacterial disease caused by toxigenic strains of Corynebacterium diphtheriae whose local and systemic manifestations are due to the action of the diphtheria toxin (DT). The vaccine which is used to prevent diphtheria worldwide is a toxoid obtained by detoxifying DT. Although associated with high efficacy in the prevention of disease, the current anti- diphtheria vaccine, one of the components of DTP (diphtheria, tetanus and pertussis triple vaccine), may present post vaccination effects such as toxicity and reactogenicity resulting from the presence of contaminants in the vaccine that originated during the process of production and/or detoxification. Therefore, strategies to develop a less toxic and at the same time economically viable vaccine alternatives are needed to improve existing vaccines in use worldwide. In this study, the Moreau substrain of BCG which is used in Brazil as a live vaccine against human tuberculosis was genetically modified to carry and express the gene encoding for the diphtheria toxin fragment B (DTB). As such, the DNA sequence encoding the dtb gene was cloned into the pUS977 shuttle vector for cytoplasmic expression and successfully introduced into BCG cells by electroporation. Mice immunized with recombinant BCG expressing DTB showed seroconversion with the detection of specific antibodies against DTB. Also, rBCGs stably expressing DTB persisted up to 60 days in the absence of selective pressure in mice and cell viability did not change significantly during the period tested. Finally, immune sera from BALB/c mice vaccinated with rBCGpUS977dtb PW8 were preliminarily tested for their capacity of neutralizing the diphtheria toxin in the Vero Cells assay.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24909/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgeraldo_armoa_etal_IOC_2017.pdfgeraldo_armoa_etal_IOC_2017.pdfapplication/pdf715759https://www.arca.fiocruz.br/bitstream/icict/24909/2/geraldo_armoa_etal_IOC_2017.pdf233c3857f976dd336cedb7de9789c154MD52TEXTgeraldo_armoa_etal_IOC_2017.pdf.txtgeraldo_armoa_etal_IOC_2017.pdf.txtExtracted texttext/plain40656https://www.arca.fiocruz.br/bitstream/icict/24909/3/geraldo_armoa_etal_IOC_2017.pdf.txt2232488f95f624e57e97f5ae774b2c7dMD53icict/249092023-09-05 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dc.title.pt_BR.fl_str_mv Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
title Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
spellingShingle Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
Nascimento, Dilzamar V.
Toxina Diftérica
Corynebacterium diphtheriae
Difteria
Mycobacterium bovis BCG
Recombinant BCG
Diphtheria Toxin dtb Gene
Park Williams 8 (PW8)
Corynebacterium diphtheriae
rDTBPW8
pUS977 Vector
title_short Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
title_full Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
title_fullStr Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
title_full_unstemmed Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
title_sort Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
author Nascimento, Dilzamar V.
author_facet Nascimento, Dilzamar V.
Dellagostin, Odir A.
Matos, Denise C. S.
McIntosh, Douglas
Hirata Jr., Raphael
Pereira, Geraldo M. B.
Guaraldi, Ana Luiza de Mattos
Armôa, Geraldo R. G.
author_role author
author2 Dellagostin, Odir A.
Matos, Denise C. S.
McIntosh, Douglas
Hirata Jr., Raphael
Pereira, Geraldo M. B.
Guaraldi, Ana Luiza de Mattos
Armôa, Geraldo R. G.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nascimento, Dilzamar V.
Dellagostin, Odir A.
Matos, Denise C. S.
McIntosh, Douglas
Hirata Jr., Raphael
Pereira, Geraldo M. B.
Guaraldi, Ana Luiza de Mattos
Armôa, Geraldo R. G.
dc.subject.other.pt_BR.fl_str_mv Toxina Diftérica
Corynebacterium diphtheriae
Difteria
Mycobacterium bovis BCG
topic Toxina Diftérica
Corynebacterium diphtheriae
Difteria
Mycobacterium bovis BCG
Recombinant BCG
Diphtheria Toxin dtb Gene
Park Williams 8 (PW8)
Corynebacterium diphtheriae
rDTBPW8
pUS977 Vector
dc.subject.en.pt_BR.fl_str_mv Recombinant BCG
Diphtheria Toxin dtb Gene
Park Williams 8 (PW8)
Corynebacterium diphtheriae
rDTBPW8
pUS977 Vector
description Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-22T12:12:49Z
dc.date.available.fl_str_mv 2018-02-22T12:12:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NASCIMENTO, Dilzamar V. et al. Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin. American Journal of Molecular Biology, v.7, p.176-189, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24909
dc.identifier.issn.pt_BR.fl_str_mv 2161-6620
dc.identifier.eissn.none.fl_str_mv 2161-6663
identifier_str_mv NASCIMENTO, Dilzamar V. et al. Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin. American Journal of Molecular Biology, v.7, p.176-189, 2017.
2161-6620
2161-6663
url https://www.arca.fiocruz.br/handle/icict/24909
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Scientific Research Publishing Inc
publisher.none.fl_str_mv Scientific Research Publishing Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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