Planarians as models to investigate the bioactivity of gold(I) complexes in vivo

Detalhes bibliográficos
Autor(a) principal: Tunes, Luiza Guimarães
Data de Publicação: 2018
Outros Autores: Allen, John M., Zayas, Ricardo M., Monte Neto, Rubens Lima do
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33789
Resumo: 2020-01-01
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spelling Tunes, Luiza GuimarãesAllen, John M.Zayas, Ricardo M.Monte Neto, Rubens Lima do2019-07-03T14:08:31Z2019-07-03T14:08:31Z2018TUNES, Luiza Guimarães et al. Planarians as models to investigate the bioactivity of gold(I) complexes in vivo. Scientific Reports, v. 8, p. 1-8, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3378910.1038/s41598-018-34558-6engNature Publishing GroupGold(I)Gold(I)Planarians as models to investigate the bioactivity of gold(I) complexes in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil / Department of Biology. San Diego State University. San Diego, CA, USA.Department of Biology. San Diego State University. San Diego, CA, USA.Department of Biology. San Diego State University. San Diego, CA, USA.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Gold(I)-containing complexes are used in drug discovery research for rheumatoid arthritis, cancer, and parasitic infections. In this study, we tested the bioactivity of gold(I) complexes in vivo using planarians. The planarian Schmidtea mediterranea possesses orthologues of tumor suppressor genes, such as p53, that, when silenced, cause deregulation of cell proliferation and apoptosis. In this context, we tested two triethylphosphine-gold(I) complexes (AdO and AdT) to determine if they can attenuate phenotypes that result from p53 inhibition. First, we identified the drug concentration that did not affect survival or regeneration and evaluated the drug’s effect on cell division and apoptosis. We found that AdT treatment decreased the number of mitotic cells and that all drug treatments increased the number of apoptotic cells. We then performed p53(RNAi) and drug treatments concomitantly and observed the phenotype progression. Drug treatment increased survival three-fold and decreased apoptosis, which resulted in an attenuated phenotype. Our results indicate that planarians can be treated with gold(I) complexes, and that this treatment can diminish the p53(RNAi) phenotype and extend survival. In this work we show that planarians can be used as a model to study the in vivo effect of gold(I) complexes and to further investigate their mechanisms of action.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33789/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALPlanarians as models to investigate the bioactivity of gold.pdfPlanarians as models to investigate the bioactivity of gold.pdfapplication/pdf4891814https://www.arca.fiocruz.br/bitstream/icict/33789/2/Planarians%20as%20models%20to%20investigate%20the%20bioactivity%20of%20gold.pdf1dec2fc1dcfd5bbc04d065d15ec3e133MD52TEXTPlanarians as models to investigate the bioactivity of gold.pdf.txtPlanarians as models to investigate the bioactivity of gold.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
title Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
spellingShingle Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
Tunes, Luiza Guimarães
Gold(I)
Gold(I)
title_short Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
title_full Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
title_fullStr Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
title_full_unstemmed Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
title_sort Planarians as models to investigate the bioactivity of gold(I) complexes in vivo
author Tunes, Luiza Guimarães
author_facet Tunes, Luiza Guimarães
Allen, John M.
Zayas, Ricardo M.
Monte Neto, Rubens Lima do
author_role author
author2 Allen, John M.
Zayas, Ricardo M.
Monte Neto, Rubens Lima do
author2_role author
author
author
dc.contributor.author.fl_str_mv Tunes, Luiza Guimarães
Allen, John M.
Zayas, Ricardo M.
Monte Neto, Rubens Lima do
dc.subject.other.pt_BR.fl_str_mv Gold(I)
topic Gold(I)
Gold(I)
dc.subject.en.pt_BR.fl_str_mv Gold(I)
description 2020-01-01
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-07-03T14:08:31Z
dc.date.available.fl_str_mv 2019-07-03T14:08:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TUNES, Luiza Guimarães et al. Planarians as models to investigate the bioactivity of gold(I) complexes in vivo. Scientific Reports, v. 8, p. 1-8, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33789
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-018-34558-6
identifier_str_mv TUNES, Luiza Guimarães et al. Planarians as models to investigate the bioactivity of gold(I) complexes in vivo. Scientific Reports, v. 8, p. 1-8, 2018.
2045-2322
10.1038/s41598-018-34558-6
url https://www.arca.fiocruz.br/handle/icict/33789
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33789/1/license.txt
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