Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test

Detalhes bibliográficos
Autor(a) principal: Tomich, Renata Graça Pinto
Data de Publicação: 2007
Outros Autores: Bomfim, Maria Rosa Quaresma, Koury, Matilde Cota, Pellegrini, Aiesca Oliveira, Pellegrin, Luiz Alberto, Ko, Albert Icksang, Stancioli, Edel Figueiredo Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11987
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil / Empresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, Brasil
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spelling Tomich, Renata Graça PintoBomfim, Maria Rosa QuaresmaKoury, Matilde CotaPellegrini, Aiesca OliveiraPellegrin, Luiz AlbertoKo, Albert IcksangStancioli, Edel Figueiredo Barbosa2015-10-19T14:32:13Z2015-10-19T14:32:13Z2007TOMICH, R. G. P. et al. Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test. Brazilian Journal of Microbiology, v. 38, p. 674-680, 2007.1517-8382https://www.arca.fiocruz.br/handle/icict/11987engSociedade Brasileira de MicrobiologiaLeptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination testinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil / Empresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilEmpresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, BrasilEmpresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Diseases. New York, USAUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilThis investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region’s climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutination test (MAT) and additionally contribute to the knowledge of the distribution of the one of most important worldwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an important ecological preserved area, where cattle constitute not only the most important economic resource but also the major activity compatible of the conservation of natural resource of the region. Out of 282 samples of cattle serum analyzed, 143 (50.71%) were positive in MAT. The serovar Hardjo (genotypic Hardjoprajitno and Hardjobovis), Wolffi and Ballum showed the largest frequency of reactive samples. In the IgG ELISA rLipL32, 161 samples (57.09%) were positive. This result was higher than obtained by MAT (p<0.001). The sensitivity of the ELISA test was 99.30% and the specificity was 86.33%, based on the MAT. This test was shown to be a more sensitive, specific and accurate test for the diagnosis of bovine leptospirosis compared to the MAT.Bovine Leptospirosis,IgG ELISA rLipL32MATinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11987/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALTomich RGP Leptospirosis serosurvey in bovines....pdfTomich RGP Leptospirosis serosurvey in bovines....pdfapplication/pdf253744https://www.arca.fiocruz.br/bitstream/icict/11987/2/Tomich%20RGP%20Leptospirosis%20serosurvey%20in%20bovines....pdf5795ecdee3fc85e82078c8a4bac7159cMD52TEXTTomich RGP Leptospirosis serosurvey in bovines....pdf.txtTomich RGP Leptospirosis serosurvey in bovines....pdf.txtExtracted texttext/plain34103https://www.arca.fiocruz.br/bitstream/icict/11987/3/Tomich%20RGP%20Leptospirosis%20serosurvey%20in%20bovines....pdf.txtbe20ec00de4f18370c984db1b86e3ee7MD53icict/119872023-03-15 14:33:07.447oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
title Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
spellingShingle Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
Tomich, Renata Graça Pinto
Bovine Leptospirosis,
IgG ELISA rLipL32
MAT
title_short Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
title_full Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
title_fullStr Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
title_full_unstemmed Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
title_sort Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test
author Tomich, Renata Graça Pinto
author_facet Tomich, Renata Graça Pinto
Bomfim, Maria Rosa Quaresma
Koury, Matilde Cota
Pellegrini, Aiesca Oliveira
Pellegrin, Luiz Alberto
Ko, Albert Icksang
Stancioli, Edel Figueiredo Barbosa
author_role author
author2 Bomfim, Maria Rosa Quaresma
Koury, Matilde Cota
Pellegrini, Aiesca Oliveira
Pellegrin, Luiz Alberto
Ko, Albert Icksang
Stancioli, Edel Figueiredo Barbosa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tomich, Renata Graça Pinto
Bomfim, Maria Rosa Quaresma
Koury, Matilde Cota
Pellegrini, Aiesca Oliveira
Pellegrin, Luiz Alberto
Ko, Albert Icksang
Stancioli, Edel Figueiredo Barbosa
dc.subject.en.pt_BR.fl_str_mv Bovine Leptospirosis,
IgG ELISA rLipL32
MAT
topic Bovine Leptospirosis,
IgG ELISA rLipL32
MAT
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil / Empresa Brasileira de Pesquisa Agropecuária. Embrapa Pantanal. Corumbá, MS, Brasil
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2015-10-19T14:32:13Z
dc.date.available.fl_str_mv 2015-10-19T14:32:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TOMICH, R. G. P. et al. Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test. Brazilian Journal of Microbiology, v. 38, p. 674-680, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11987
dc.identifier.issn.none.fl_str_mv 1517-8382
identifier_str_mv TOMICH, R. G. P. et al. Leptospirosis serosurvey in bovines from Brazilian pantanal using IGG Elisa with recombinant protein LIPL32 and microscopic agglulutination test. Brazilian Journal of Microbiology, v. 38, p. 674-680, 2007.
1517-8382
url https://www.arca.fiocruz.br/handle/icict/11987
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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