The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia

Detalhes bibliográficos
Autor(a) principal: Almeida-Siva, Fernando
Data de Publicação: 2023
Outros Autores: Almeida-Paes, Rodrigo, Serra-Damasceno, Lisandra, Motta-Santos, Edwiges, Ferreira, Luiz Claudio, Pereira-Quintella, Leonardo, Ferreira, Marcela de Faria, Medeiros-Muniz, Mauro de, Zancopé-Oliveira, Rosely M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/61090
Resumo: This research was funded by Fundação de Amparo a Pesquisa do Estado do Rio de Ja-neiro (FAPERJ), grant number E-26/203.076/2016. R.M.Z-O. It was also supported in part by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 302796/2017-7) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) E-26/202.527/2019.
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spelling Almeida-Siva, FernandoAlmeida-Paes, RodrigoSerra-Damasceno, LisandraMotta-Santos, EdwigesFerreira, Luiz ClaudioPereira-Quintella, LeonardoFerreira, Marcela de FariaMedeiros-Muniz, Mauro deZancopé-Oliveira, Rosely M.2023-11-08T00:05:53Z2023-11-08T00:05:53Z2023ALMEIDA-SILVA, Fernando et al. The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia. Biomédica: Revista del Instituto Nacional de Salud, v. 43, Sp. 1, p. 255-266, Aug. 2023.0120-4157https://www.arca.fiocruz.br/handle/icict/6109010.7705/biomedica.70202590-7379This research was funded by Fundação de Amparo a Pesquisa do Estado do Rio de Ja-neiro (FAPERJ), grant number E-26/203.076/2016. R.M.Z-O. It was also supported in part by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 302796/2017-7) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) E-26/202.527/2019.Introducción: Pneumocystis jirovecii es un hongo oportunista que afecta principalmente a personas con HIV (recuento de CD4 menor de 200 células/ml) y a otros pacientes inmunosuprimidos. Como P. jirovecii no crece en los medios micológicos de rutina, el diagnóstico de neumonía por P. jirovecii se basa en la evidencia presente en muestras respiratorias. Objetivos: Asociar los resultados de la inmunofluorescencia directa y los de dos métodos moleculares con un puntaje para predecir la neumonía causada por P. jirovecii en pacientes con sida. Materiales y métodos: Se realizó un estudio prospectivo de 40 pacientes. Se recolectó una muestra respiratoria antes del inicio de tratamiento y se sometió a una prueba de inmunofluorescencia directa con el kit Merifluor, una PCR anidada para la amplificación de la subunidad larga del ribosoma mitocondrial y una PCR en tiempo real usando el kit VIASURE. Resultados: Estas tres técnicas evidenciaron la presencia de P. jirovecii en 6, 12 y 15 muestras, respectivamente. Todas las muestras positivas por inmunofluorescencia directa fueron positivas en la PCR anidada y todas las muestras positivas en la PCR anidada amplificaron por PCR en tiempo real. Se encontró una asociación estadística entre los valores de la neumonía causada por P. jirovecii y los métodos moleculares. Dos pacientes con diagnóstico temprano respondieron satisfactoriamente al tratamiento. Conclusión. Se recomiendan los métodos moleculares, especialmente la PCR en tiempo real, para el diagnóstico temprano de neumonía causada por P. jirovecii en pacientes con sida. reacción en cadena en tiempo real de la polimerasa.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brazil.Hospital São José de Doenças Infecciosas. Fortaleza, CE, Brazil / Universidade Federal do Ceará. Faculdade de Medicina. Departamento de Saúde Comunitária. Fortaleza, CE, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço de Anatomia Patológica. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço Ambulatorial. Rio de Janeiro, RJ, Brazil.Introduction: Pneumocystis jirovecii is an opportunistic fungus that affects mainly people living with HIV (CD4 cell count lower than 200 cells/ml) and other immunosuppressed patients. Since P. jirovecii does not grow on routine mycological media, diagnosis of P. jirovecii pneumonia relies on indirect evidence of its presence in respiratory samples. Objectives: To associate the results of direct immunofluorescence and two molecular methods with a score to predict P. jirovecii pneumonia in patients with AIDS. Materials and methods: A prospective study was conducted with 40 patients. A respiratory sample collected before treatment was subjected to direct immunofluorescence using the Merifluor kit, to nested PCR targeting the mitochondrial large subunit ribosomal RNA, and to the VIASURE real-time PCR kit. Results: These three techniques revealed P. jirovecii in 6, 12, and 15 samples, respectively. All positive samples by direct immunofluorescence were positive by nested PCR, and all positive samples by nested PCR amplified by real-time PCR. There was a statistically significant association between the P. jirovecii pneumonia score and the molecular methods. Two patients were early diagnosed and responded well to treatment. Conclusion: Molecular methods, especially real-time PCR, are recommended for early diagnosis of P. jirovecii pneumonia in AIDS patients.engEl InstitutoThe conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumoniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePneumonia pneumocystisFluorescent antibody technique directReal-time polymerase chain reactionNeumonía por PneumocystisTécnica de inmunofluorescencia directaReacción en cadena en tiempo real de la polimerasainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
title The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
spellingShingle The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
Almeida-Siva, Fernando
Pneumonia pneumocystis
Fluorescent antibody technique direct
Real-time polymerase chain reaction
Neumonía por Pneumocystis
Técnica de inmunofluorescencia directa
Reacción en cadena en tiempo real de la polimerasa
title_short The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
title_full The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
title_fullStr The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
title_full_unstemmed The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
title_sort The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
author Almeida-Siva, Fernando
author_facet Almeida-Siva, Fernando
Almeida-Paes, Rodrigo
Serra-Damasceno, Lisandra
Motta-Santos, Edwiges
Ferreira, Luiz Claudio
Pereira-Quintella, Leonardo
Ferreira, Marcela de Faria
Medeiros-Muniz, Mauro de
Zancopé-Oliveira, Rosely M.
author_role author
author2 Almeida-Paes, Rodrigo
Serra-Damasceno, Lisandra
Motta-Santos, Edwiges
Ferreira, Luiz Claudio
Pereira-Quintella, Leonardo
Ferreira, Marcela de Faria
Medeiros-Muniz, Mauro de
Zancopé-Oliveira, Rosely M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida-Siva, Fernando
Almeida-Paes, Rodrigo
Serra-Damasceno, Lisandra
Motta-Santos, Edwiges
Ferreira, Luiz Claudio
Pereira-Quintella, Leonardo
Ferreira, Marcela de Faria
Medeiros-Muniz, Mauro de
Zancopé-Oliveira, Rosely M.
dc.subject.en.en_US.fl_str_mv Pneumonia pneumocystis
Fluorescent antibody technique direct
Real-time polymerase chain reaction
topic Pneumonia pneumocystis
Fluorescent antibody technique direct
Real-time polymerase chain reaction
Neumonía por Pneumocystis
Técnica de inmunofluorescencia directa
Reacción en cadena en tiempo real de la polimerasa
dc.subject.es.en_US.fl_str_mv Neumonía por Pneumocystis
Técnica de inmunofluorescencia directa
Reacción en cadena en tiempo real de la polimerasa
description This research was funded by Fundação de Amparo a Pesquisa do Estado do Rio de Ja-neiro (FAPERJ), grant number E-26/203.076/2016. R.M.Z-O. It was also supported in part by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 302796/2017-7) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) E-26/202.527/2019.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-11-08T00:05:53Z
dc.date.available.fl_str_mv 2023-11-08T00:05:53Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA-SILVA, Fernando et al. The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia. Biomédica: Revista del Instituto Nacional de Salud, v. 43, Sp. 1, p. 255-266, Aug. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/61090
dc.identifier.issn.en_US.fl_str_mv 0120-4157
dc.identifier.doi.none.fl_str_mv 10.7705/biomedica.7020
dc.identifier.eissn.none.fl_str_mv 2590-7379
identifier_str_mv ALMEIDA-SILVA, Fernando et al. The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia. Biomédica: Revista del Instituto Nacional de Salud, v. 43, Sp. 1, p. 255-266, Aug. 2023.
0120-4157
10.7705/biomedica.7020
2590-7379
url https://www.arca.fiocruz.br/handle/icict/61090
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv El Instituto
publisher.none.fl_str_mv El Instituto
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/61090/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/61090/2/ve_Fernando_Almeida-Silva_etal_INI_2023.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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