Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14295 http://dx.doi.org/10.1155/2014/981923 |
Resumo: | Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil. / Brazilian Institute of Environment and Renewable Natural Resources. Porto Velho, RO, Brazil. |
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Guimarães, C. L. S.Andrião-Escarso, S. H.Moreira-Dill, L. S.Carvalho, B. M. A.Marchi-Salvador, D. P.Santos-Filho, N. A.Fernandes, C. A. H.Fontes, M. R. M.Giglio, J. R.Barraviera, B.Zuliani, J. P.Fernandes, C. F. C.Calderón, L. A.Stábeli, R. G.Albericio, F.Silva, S. L. daSoares, A. M.2016-05-16T14:07:41Z2016-05-16T14:07:41Z2014GUIMARÃES, C. L. S. et al. Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases A2: A Biotechnological Tool to Improve the Production of Antibodies. BioMed Research International, v. 2014, p. 1-12, 2014.https://www.arca.fiocruz.br/handle/icict/14295http://dx.doi.org/10.1155/2014/981923engBioMed Research InternationalAlkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil. / Brazilian Institute of Environment and Renewable Natural Resources. Porto Velho, RO, Brazil.University of São Paulo. Faculty of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brazil.Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil.Federal University of São Joao Del Rei. Department of Chemistry, Biotechnology and Bioprocess Engineering. Ouro Branco, MG, Brazil.Federal University of Paraíba. Center for Science and Nature. Department of Molecular Biology. João Pessoa, Brazil.University of São Paulo. Faculty of Pharmaceutical Sciences of Ribeirão Preto. Department of Clinical Analysis. Ribeirão Preto, SP, Brazil.State University Paulista. Department of Physics and Biophysics. Botucatu, SP, Brazil.State University Paulista. Department of Physics and Biophysics. Botucatu, SP, Brazil.University of São Paulo. Faculty of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brazil.State University Paulista. Center for the Study of Venoms and Venomous Animals. Botucatu, SP, Brazil.Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil.Barcelona Science Park. Institute for Research in Biomedicine and CIBER-BBN. Barcelona, Spain. / University of Barcelona. Department of Organic Chemistry. Barcelona, Spain. / University of KwaZulu Natal. School of Chemistry and Physics. Durban, South Africa.Federal University of São Joao Del Rei. Department of Chemistry, Biotechnology and Bioprocess Engineering. Ouro Branco, MG, Brazil.Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil.Crude venom of Bothrops jararacussu and isolated phospholipases A2 (PLA2) of this toxin (BthTX-I and BthTX-II) were chemically modified (alkylation) by p-bromophenacyl bromide (BPB) in order to study antibody production capacity in function of the structure-function relationship of these substances (crude venom and PLA2 native and alkylated). BthTX-II showed enzymatic activity, while BthTX-I did not. Alkylation reduced BthTX-II activity by 50% while this process abolished the catalytic and myotoxic activities of BthTX-I, while reducing its edema-inducing activity by about 50%. Antibody production against the native and alkylated forms of BthTX-I and -II and the cross-reactivity of antibodies to native and alkylated toxins did not show any apparent differences and these observations were reinforced by surface plasmon resonance (SPR) data. Histopathological analysis of mouse gastrocnemius muscle sections after injection of PBS, BthTX-I, BthTX-II, or both myotoxins previously incubated with neutralizing antibody showed inhibition of the toxin-inducedmyotoxicity.These results reveal that the chemical modification of the phospholipases A2 (PLA2) diminished their toxicity but did not alter their antigenicity. This observation indicates that themodified PLA2 may provide a biotechnological tool to attenuate the toxicity of the crude venom, by improving the production of antibodies and decreasing the local toxic effects of this poisonous substance in animals used to produce antivenom.Bothrops jararacuçuPhospholipases A2Venom Proteinsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14295/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlkylation of Histidine Residues of Bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies.pdfAlkylation of Histidine Residues of Bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies.pdfapplication/pdf3055761https://www.arca.fiocruz.br/bitstream/icict/14295/2/Alkylation%20of%20Histidine%20Residues%20of%20Bothrops%20jararacussu%20venom%20proteins%20and%20isolated%20phospholipases%20A2%20a%20biotechnological%20tool%20to%20improve%20the%20production%20of%20antibodies.pdfccac03d4311a62188145b676dc9d0f34MD52TEXTAlkylation of Histidine Residues of Bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies.pdf.txtAlkylation of Histidine Residues of Bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies |
title |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies |
spellingShingle |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies Guimarães, C. L. S. Bothrops jararacuçu Phospholipases A2 Venom Proteins |
title_short |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies |
title_full |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies |
title_fullStr |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies |
title_full_unstemmed |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies |
title_sort |
Alkylation of histidine residues of bothrops jararacussu venom proteins and isolated phospholipases A2 a biotechnological tool to improve the production of antibodies |
author |
Guimarães, C. L. S. |
author_facet |
Guimarães, C. L. S. Andrião-Escarso, S. H. Moreira-Dill, L. S. Carvalho, B. M. A. Marchi-Salvador, D. P. Santos-Filho, N. A. Fernandes, C. A. H. Fontes, M. R. M. Giglio, J. R. Barraviera, B. Zuliani, J. P. Fernandes, C. F. C. Calderón, L. A. Stábeli, R. G. Albericio, F. Silva, S. L. da Soares, A. M. |
author_role |
author |
author2 |
Andrião-Escarso, S. H. Moreira-Dill, L. S. Carvalho, B. M. A. Marchi-Salvador, D. P. Santos-Filho, N. A. Fernandes, C. A. H. Fontes, M. R. M. Giglio, J. R. Barraviera, B. Zuliani, J. P. Fernandes, C. F. C. Calderón, L. A. Stábeli, R. G. Albericio, F. Silva, S. L. da Soares, A. M. |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Guimarães, C. L. S. Andrião-Escarso, S. H. Moreira-Dill, L. S. Carvalho, B. M. A. Marchi-Salvador, D. P. Santos-Filho, N. A. Fernandes, C. A. H. Fontes, M. R. M. Giglio, J. R. Barraviera, B. Zuliani, J. P. Fernandes, C. F. C. Calderón, L. A. Stábeli, R. G. Albericio, F. Silva, S. L. da Soares, A. M. |
dc.subject.en.pt_BR.fl_str_mv |
Bothrops jararacuçu Phospholipases A2 Venom Proteins |
topic |
Bothrops jararacuçu Phospholipases A2 Venom Proteins |
description |
Fundação Oswaldo Cruz. Centro de Estudos Aplicados de Biomoléculas em Saúde. Porto Velho, RO, Brazil. / Federal University of Rondônia. Department of Medicine. Porto Velho, RO, Brazil. / Brazilian Institute of Environment and Renewable Natural Resources. Porto Velho, RO, Brazil. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2016-05-16T14:07:41Z |
dc.date.available.fl_str_mv |
2016-05-16T14:07:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GUIMARÃES, C. L. S. et al. Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases A2: A Biotechnological Tool to Improve the Production of Antibodies. BioMed Research International, v. 2014, p. 1-12, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/14295 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1155/2014/981923 |
identifier_str_mv |
GUIMARÃES, C. L. S. et al. Alkylation of Histidine Residues of Bothrops jararacussu Venom Proteins and Isolated Phospholipases A2: A Biotechnological Tool to Improve the Production of Antibodies. BioMed Research International, v. 2014, p. 1-12, 2014. |
url |
https://www.arca.fiocruz.br/handle/icict/14295 http://dx.doi.org/10.1155/2014/981923 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
BioMed Research International |
publisher.none.fl_str_mv |
BioMed Research International |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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