Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection

Detalhes bibliográficos
Autor(a) principal: Ricciardi-Jorge, Taissa
Data de Publicação: 2017
Outros Autores: Bordignon, Juliano, Koishi, Andrea Cristine, Zanluca, Camila, Mosimann, Ana Luiza Pamplona, Santos, Claudia Nunes Duarte dos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26214
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
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spelling Ricciardi-Jorge, TaissaBordignon, JulianoKoishi, Andrea CristineZanluca, CamilaMosimann, Ana Luiza PamplonaSantos, Claudia Nunes Duarte dos2018-05-02T14:59:59Z2018-05-02T14:59:59Z2017RICCIARDI-JORGE, Taissa et al. Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection. Scientific Reports, v. 7, n. 16229, p. 1-8, 2017.2045-2322https://www.arca.fiocruz.br/handle/icict/2621410.1038/s41598-017-16231-610.1038/s41598-017-16231-6porNature Publishing GroupDiagnósticoYellow FeverDiagnosisEnzyme-Linked Immunosorbent AssayFiebre AmarillaEnsayo de Inmunoadsorción EnzimáticaFebre AmarelaEnsaio de Imunoadsorção EnzimáticaDevelopment of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Yellow fever is an arboviral disease that causes thousands of deaths every year in Africa and the Americas. However, few commercial diagnostic kits are available. Non-structural protein 1 (NS1) is an early marker of several flavivirus infections and is widely used to diagnose dengue virus (DENV) infection. Nonetheless, little is known about the dynamics of Yellow fever virus (YFV) NS1 expression and secretion, to encourage its use in diagnosis. To tackle this issue, we developed a quantitative NS1-capture ELISA specific for YFV using a monoclonal antibody and recombinant NS1 protein. This test was used to quantify NS1 in mosquito and human cell line cultures infected with vaccine and wild YFV strains. Our results showed that NS1 was detectable in the culture supernatants of both cell lines; however, a higher concentration was maintained as cell-associated rather than secreted into the extracellular milieu. A panel of 73 human samples was used to demonstrate the suitability of YFV NS1 as a diagnostic tool, resulting in 80% sensitivity, 100% specificity, a 100% positive predictive value and a 95.5% negative predictive value compared with RT-PCR. Overall, the developed NS1-capture ELISA showed potential as a promising assay for the detection of early YF infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/26214/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALtaissaDevelopment.pdftaissaDevelopment.pdfapplication/pdf2289648https://www.arca.fiocruz.br/bitstream/icict/26214/2/taissaDevelopment.pdfe06564731f7c627430412daf18e48364MD52TEXTtaissaDevelopment.pdf.txttaissaDevelopment.pdf.txtExtracted texttext/plain35804https://www.arca.fiocruz.br/bitstream/icict/26214/3/taissaDevelopment.pdf.txt076de7d79900402c1d5c793c75c82289MD53icict/262142019-11-23 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dc.title.pt_BR.fl_str_mv Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
title Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
spellingShingle Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
Ricciardi-Jorge, Taissa
Diagnóstico
Yellow Fever
Diagnosis
Enzyme-Linked Immunosorbent Assay
Fiebre Amarilla
Ensayo de Inmunoadsorción Enzimática
Febre Amarela
Ensaio de Imunoadsorção Enzimática
title_short Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
title_full Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
title_fullStr Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
title_full_unstemmed Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
title_sort Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection
author Ricciardi-Jorge, Taissa
author_facet Ricciardi-Jorge, Taissa
Bordignon, Juliano
Koishi, Andrea Cristine
Zanluca, Camila
Mosimann, Ana Luiza Pamplona
Santos, Claudia Nunes Duarte dos
author_role author
author2 Bordignon, Juliano
Koishi, Andrea Cristine
Zanluca, Camila
Mosimann, Ana Luiza Pamplona
Santos, Claudia Nunes Duarte dos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ricciardi-Jorge, Taissa
Bordignon, Juliano
Koishi, Andrea Cristine
Zanluca, Camila
Mosimann, Ana Luiza Pamplona
Santos, Claudia Nunes Duarte dos
dc.subject.other.pt_BR.fl_str_mv Diagnóstico
topic Diagnóstico
Yellow Fever
Diagnosis
Enzyme-Linked Immunosorbent Assay
Fiebre Amarilla
Ensayo de Inmunoadsorción Enzimática
Febre Amarela
Ensaio de Imunoadsorção Enzimática
dc.subject.en.pt_BR.fl_str_mv Yellow Fever
Diagnosis
Enzyme-Linked Immunosorbent Assay
dc.subject.es.pt_BR.fl_str_mv Fiebre Amarilla
Ensayo de Inmunoadsorción Enzimática
dc.subject.decs.pt_BR.fl_str_mv Febre Amarela
Ensaio de Imunoadsorção Enzimática
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-05-02T14:59:59Z
dc.date.available.fl_str_mv 2018-05-02T14:59:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RICCIARDI-JORGE, Taissa et al. Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection. Scientific Reports, v. 7, n. 16229, p. 1-8, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26214
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.pt_BR.fl_str_mv 10.1038/s41598-017-16231-6
dc.identifier.eissn.none.fl_str_mv 10.1038/s41598-017-16231-6
identifier_str_mv RICCIARDI-JORGE, Taissa et al. Development of a quantitative NS1-capture enzyme-linked immunosorbent assay for early detection of yellow fever virus infection. Scientific Reports, v. 7, n. 16229, p. 1-8, 2017.
2045-2322
10.1038/s41598-017-16231-6
url https://www.arca.fiocruz.br/handle/icict/26214
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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