Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1371/journal.pone.0021289 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59695 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil |
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Bueno, Lilian LacerdaLobo, Francisco PereiraMorais, Cristiane GuimaraesMourao, Luıza CarvalhoAvila, Ricardo Andrez Machado deSoares, Irene SilvaFontes, Cor JesusLacerda, Marcus ViníciusOlortegui, Carlos ChavezBartholomeu, Daniella CastanheiraFujiwara, Ricardo ToshioBraga, Erika Martins2023-07-20T16:30:08Z2023-07-20T16:30:08Z2011BUENO, Lilian Lacerda et al. Identification of a highly antigenic linear B cell epitope within Plasmodium vivax apical membrane antigen 1 (AMA-1). PLoS One, v. 6, n. 6, e21289, 2011. doi: 10.1371/journal.pone.0021289.1935-2735https://www.arca.fiocruz.br/handle/icict/5969510.1371/journal.pone.0021289engPublic Library of ScienceIdentification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade de São Paulo. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, BrazilUniversidade Federal de Mato Grosso. Departamento de Clınica Medica. Cuiaba, MT, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil/Instituto Nacional de Ciência e Tecnologia de Vacinas. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrazilApical membrane antigen 1 (AMA-1) is considered to be a major candidate antigen for a malaria vaccine. Previous immunoepidemiological studies of naturally acquired immunity to Plasmodium vivax AMA-1 (PvAMA-1) have shown a higher prevalence of specific antibodies to domain II (DII) of AMA-1. In the present study, we confirmed that specific antibody responses from naturally infected individuals were highly reactive to both full-length AMA-1 and DII. Also, we demonstrated a strong association between AMA-1 and DII IgG and IgG subclass responses. We analyzed the primary sequence of PvAMA-1 for B cell linear epitopes co-occurring with intrinsically unstructured/ disordered regions (IURs). The B cell epitope comprising the amino acid sequence 290-307 of PvAMA-1 (SASDQPTQYEEEMTDYQK), with the highest prediction scores, was identified in domain II and further selected for chemical synthesis and immunological testing. The antigenicity of the synthetic peptide was identified by serological analysis using sera from P. vivax-infected individuals who were knowingly reactive to the PvAMA-1 ectodomain only, domain II only, or reactive to both antigens. Although the synthetic peptide was recognized by all serum samples specific to domain II, serum with reactivity only to the full-length protein presented 58.3% positivity. Moreover, IgG reactivity against PvAMA-1 and domain II after depletion of specific synthetic peptide antibodies was reduced by 18% and 33% (P = 0.0001 for both), respectively. These results suggest that the linear epitope SASDQPTQYEEEMTDYQK is highly antigenic during natural human infections and is an important antigenic region of the domain II of PvAMA-1, suggesting its possible future use in pre-clinical studiesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALpone.0021289 1..9.pdfpone.0021289 1..9.pdfapplication/pdf465572https://www.arca.fiocruz.br/bitstream/icict/59695/2/pone.0021289%201..9.pdfef9a1da0da9ac7b269e397ae59e93842MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59695/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/596952023-07-20 13:30:08.789oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-20T16:30:08Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) |
| title |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) |
| spellingShingle |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) Bueno, Lilian Lacerda |
| title_short |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) |
| title_full |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) |
| title_fullStr |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) |
| title_full_unstemmed |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) |
| title_sort |
Identification of a Highly Antigenic Linear B Cell Epitope within Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) |
| author |
Bueno, Lilian Lacerda |
| author_facet |
Bueno, Lilian Lacerda Lobo, Francisco Pereira Morais, Cristiane Guimaraes Mourao, Luıza Carvalho Avila, Ricardo Andrez Machado de Soares, Irene Silva Fontes, Cor Jesus Lacerda, Marcus Vinícius Olortegui, Carlos Chavez Bartholomeu, Daniella Castanheira Fujiwara, Ricardo Toshio Braga, Erika Martins |
| author_role |
author |
| author2 |
Lobo, Francisco Pereira Morais, Cristiane Guimaraes Mourao, Luıza Carvalho Avila, Ricardo Andrez Machado de Soares, Irene Silva Fontes, Cor Jesus Lacerda, Marcus Vinícius Olortegui, Carlos Chavez Bartholomeu, Daniella Castanheira Fujiwara, Ricardo Toshio Braga, Erika Martins |
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author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bueno, Lilian Lacerda Lobo, Francisco Pereira Morais, Cristiane Guimaraes Mourao, Luıza Carvalho Avila, Ricardo Andrez Machado de Soares, Irene Silva Fontes, Cor Jesus Lacerda, Marcus Vinícius Olortegui, Carlos Chavez Bartholomeu, Daniella Castanheira Fujiwara, Ricardo Toshio Braga, Erika Martins |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciência Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil |
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2011 |
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2011 |
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2023-07-20T16:30:08Z |
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2023-07-20T16:30:08Z |
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BUENO, Lilian Lacerda et al. Identification of a highly antigenic linear B cell epitope within Plasmodium vivax apical membrane antigen 1 (AMA-1). PLoS One, v. 6, n. 6, e21289, 2011. doi: 10.1371/journal.pone.0021289. |
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10.1371/journal.pone.0021289 |
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BUENO, Lilian Lacerda et al. Identification of a highly antigenic linear B cell epitope within Plasmodium vivax apical membrane antigen 1 (AMA-1). PLoS One, v. 6, n. 6, e21289, 2011. doi: 10.1371/journal.pone.0021289. 1935-2735 10.1371/journal.pone.0021289 |
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