Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41461 |
Resumo: | Universidade Federal de Juiz de Fora. Hospital Universitário. Departamento de Oftalmologia. Juiz de Fora, MG, Brasil. |
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Matos, Aline M. F.Moreira, Lucas M.Barczewski, Bianca F.Matos, Lucas X. deOliveira, Jordane B. V.Pimentel, Maria Ines F.Paes, Rodrigo AlmeidaOliveira, Murilo G.Pinto, Tatiana C. A.Lima, NelsonMatos, Magnum de O.Costa, Louise G. de M.Santos, CledirOliveira, Manoel Marques Evangelista2020-05-31T16:13:12Z2020-05-31T16:13:12Z2020MATOS, Aline M. E. et al. Identification by MALDI-TOF-MS of Sporothrix brasiliensis isolated from a Subconjuctival infiltrative lesion in an immunocompetent patient. Microorganisms, v. 8, n. 22, p. 1-12, 2020.2076-2607https://www.arca.fiocruz.br/handle/icict/4146110.3390/microorganisms8010022engMDPIEsporotricoseSporotrix brasiliensisSpotricose ocularIdentificação fúngicaSporotrichosisSporothrix brasiliensisOcular sporotrichosisFungal identificationMALDI-TOF-MSIdentification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patientinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Hospital Universitário. Departamento de Oftalmologia. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora. Hospital Universitário. Departamento de Oftalmologia. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Hospital Universitário. Departamento de Oftalmologia. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Hospital Universitário. Departamento de Oftalmologia. Juiz de Fora, MG Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora. Departamento de Farmácia. Juiz de Fora, MG, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goes. Rio de Janeiro, RJ, Brasil.Universidade do Minho. CEB-Biological Engineering Centre. Campus Braga, Portugal.Hospital Nove de Julho. Instituto Oncológico. Departamento de Imagem. São Paulo, SP, Brasil.Universidade Federal de Juiz de Fora. Faculdade de Medicina. Departamento de Patologia. Juiz de Fora, MG, Brasil.Universidad de La Feontera. BIOIREN-UFRO. Department of Chemical Science and Natural Resources. Temuco, Chile.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímíca e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Sporotrichosis is a globally distributed subcutaneous fungal infection caused by dimorphic fungi belonging to the Sporothrix species complex that a ects the skin of limbs predominantly, but not exclusively. A rare case of ocular sporotrichosis in an immunocompetent Brazilian patient from the countryside of Rio de Janeiro State is reported. A 68-year-old woman presented with a subconjunctival infiltrative lesion in the right eye with pre-auricular lymphadenopathy of onset 4 months ago that evolved to suppurative nodular lesions on the eyelids. Conjunctival secretion was evaluated by histopathological examination and inoculated on Sabouraud Dextrose Agar (SDA). Histopathology showed oval bodies within giant cells and other mononucleated histiocytes. Fungus grown on SDA was identified as Sporothrix sp. by morphological observations. The isolated strain was finally identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) associated with an in-house database enriched with reference Sporothrix complex spectra. The strain presented a MALDI spectrum with the ion peaks of the molecular mass profile of S. brasiliensis. The patient was adequately treated with amphotericin B subsequently replaced byAbstract: Sporotrichosis is a globally distributed subcutaneous fungal infection caused by dimorphic fungi belonging to the Sporothrix species complex that a ects the skin of limbs predominantly, but not exclusively. A rare case of ocular sporotrichosis in an immunocompetent Brazilian patient from the countryside of Rio de Janeiro State is reported. A 68-year-old woman presented with a subconjunctival infiltrative lesion in the right eye with pre-auricular lymphadenopathy of onset 4 months ago that evolved to suppurative nodular lesions on the eyelids. Conjunctival secretion was evaluated by histopathological examination and inoculated on Sabouraud Dextrose Agar (SDA). Histopathology showed oval bodies within giant cells and other mononucleated histiocytes. Fungus grown on SDA was identified as Sporothrix sp. by morphological observations. The isolated strain was finally identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) associated with an in-house database enriched with reference Sporothrix complex spectra. The strain presented a MALDI spectrum with the ion peaks of the molecular mass profile of S. brasiliensis. The patient was adequately treated with amphotericin B subsequently replaced by itraconazole. Due to scars left by the suppurative process, the patient presented poor final visual acuity. The present work presents an overview of ocular sporotrichosis and discusses the diagnostic di culty that can lead to visual sequelae in these cases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41461/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALManuelME_Oliveira_etal_IOC_2019.pdfManuelME_Oliveira_etal_IOC_2019.pdfapplication/pdf2631525https://www.arca.fiocruz.br/bitstream/icict/41461/2/ManuelME_Oliveira_etal_IOC_2019.pdf86054b4424f284ce5cf831ff1d6b62bbMD52TEXTManuelME_Oliveira_etal_IOC_2019.pdf.txtManuelME_Oliveira_etal_IOC_2019.pdf.txtExtracted texttext/plain49114https://www.arca.fiocruz.br/bitstream/icict/41461/3/ManuelME_Oliveira_etal_IOC_2019.pdf.txt090e8e1cea9808c592d4c2747e28e508MD53icict/414612020-07-23 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| dc.title.pt_BR.fl_str_mv |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient |
| title |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient |
| spellingShingle |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient Matos, Aline M. F. Esporotricose Sporotrix brasiliensis Spotricose ocular Identificação fúngica Sporotrichosis Sporothrix brasiliensis Ocular sporotrichosis Fungal identification MALDI-TOF-MS |
| title_short |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient |
| title_full |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient |
| title_fullStr |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient |
| title_full_unstemmed |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient |
| title_sort |
Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient |
| author |
Matos, Aline M. F. |
| author_facet |
Matos, Aline M. F. Moreira, Lucas M. Barczewski, Bianca F. Matos, Lucas X. de Oliveira, Jordane B. V. Pimentel, Maria Ines F. Paes, Rodrigo Almeida Oliveira, Murilo G. Pinto, Tatiana C. A. Lima, Nelson Matos, Magnum de O. Costa, Louise G. de M. Santos, Cledir Oliveira, Manoel Marques Evangelista |
| author_role |
author |
| author2 |
Moreira, Lucas M. Barczewski, Bianca F. Matos, Lucas X. de Oliveira, Jordane B. V. Pimentel, Maria Ines F. Paes, Rodrigo Almeida Oliveira, Murilo G. Pinto, Tatiana C. A. Lima, Nelson Matos, Magnum de O. Costa, Louise G. de M. Santos, Cledir Oliveira, Manoel Marques Evangelista |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Matos, Aline M. F. Moreira, Lucas M. Barczewski, Bianca F. Matos, Lucas X. de Oliveira, Jordane B. V. Pimentel, Maria Ines F. Paes, Rodrigo Almeida Oliveira, Murilo G. Pinto, Tatiana C. A. Lima, Nelson Matos, Magnum de O. Costa, Louise G. de M. Santos, Cledir Oliveira, Manoel Marques Evangelista |
| dc.subject.other.pt_BR.fl_str_mv |
Esporotricose Sporotrix brasiliensis Spotricose ocular Identificação fúngica |
| topic |
Esporotricose Sporotrix brasiliensis Spotricose ocular Identificação fúngica Sporotrichosis Sporothrix brasiliensis Ocular sporotrichosis Fungal identification MALDI-TOF-MS |
| dc.subject.en.pt_BR.fl_str_mv |
Sporotrichosis Sporothrix brasiliensis Ocular sporotrichosis Fungal identification MALDI-TOF-MS |
| description |
Universidade Federal de Juiz de Fora. Hospital Universitário. Departamento de Oftalmologia. Juiz de Fora, MG, Brasil. |
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2020 |
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2020-05-31T16:13:12Z |
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2020-05-31T16:13:12Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MATOS, Aline M. E. et al. Identification by MALDI-TOF-MS of Sporothrix brasiliensis isolated from a Subconjuctival infiltrative lesion in an immunocompetent patient. Microorganisms, v. 8, n. 22, p. 1-12, 2020. |
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https://www.arca.fiocruz.br/handle/icict/41461 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2076-2607 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/microorganisms8010022 |
| identifier_str_mv |
MATOS, Aline M. E. et al. Identification by MALDI-TOF-MS of Sporothrix brasiliensis isolated from a Subconjuctival infiltrative lesion in an immunocompetent patient. Microorganisms, v. 8, n. 22, p. 1-12, 2020. 2076-2607 10.3390/microorganisms8010022 |
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MDPI |
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MDPI |
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