Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19719 |
Resumo: | Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil. |
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Marques, Viviane FigueiraMotta, Cássia Couto daSoares, Bianca da SilvaMelo, Dayanne Araújo deCoelho, Shana de Mattos de OliveiraCoelho, Irene da SilvaBarbosa, Helene SantosSouza, Miliane Moreira Soares de2017-07-05T12:56:49Z2017-07-05T12:56:49Z2017MARQUES, Viviane Figueira; et al. Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis. Brazilian Journal of Microbiology, v.48, n.1, p.118-124, Jan.- Mar. 2017.1517-8382https://www.arca.fiocruz.br/handle/icict/1971910.1016/j.bjm.2016.10.001engSociedade Brasileira de MicrobiologiaBiofilmesMastiteResistência antimicrobianaStaphylococcus aureusBiofilmAgr typesAntimicrobial resistanceMastitisStaphylococcus aureusBiofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica. Instituto de Veterinária (IV).Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil.Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to determine the most suitable time interval for biofilm analysis, scanning electron microscopy was performed. Furthermore, genes such as mecA and blaZ that are related to beta-lactamic resistance and oxacillin susceptibility were tested. All the studied isolates were biofilm producers and mostly presented icaA and icaD. The Agr type II genes were significantly prevalent. According to the SEM, gradual changes in the bacterial arrangement were observed during biofilm formation along the growth curve phases, and the peak was reached at the stationary phase. In this study, the penicillin resistance was related to the production of beta-lactamase, and the high minimal bactericidal concentration for cefoxitin was possibly associated with biofilm protection. Therefore, further studies are warranted to better understand biofilm formation, possibly contributing to our knowledge about bacterial resistance in vivo.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19719/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALhelene_barbosa_etal_IOC_2017.pdfhelene_barbosa_etal_IOC_2017.pdfapplication/pdf2216959https://www.arca.fiocruz.br/bitstream/icict/19719/2/helene_barbosa_etal_IOC_2017.pdf4e795341de226c742692ef9385f93e6fMD52TEXThelene_barbosa_etal_IOC_2017.pdf.txthelene_barbosa_etal_IOC_2017.pdf.txtExtracted texttext/plain36206https://www.arca.fiocruz.br/bitstream/icict/19719/3/helene_barbosa_etal_IOC_2017.pdf.txta4d29714806acb0e119aabab7c5c27f2MD53icict/197192018-08-15 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| dc.title.pt_BR.fl_str_mv |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
| title |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
| spellingShingle |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis Marques, Viviane Figueira Biofilmes Mastite Resistência antimicrobiana Staphylococcus aureus Biofilm Agr types Antimicrobial resistance Mastitis Staphylococcus aureus |
| title_short |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
| title_full |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
| title_fullStr |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
| title_full_unstemmed |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
| title_sort |
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis |
| author |
Marques, Viviane Figueira |
| author_facet |
Marques, Viviane Figueira Motta, Cássia Couto da Soares, Bianca da Silva Melo, Dayanne Araújo de Coelho, Shana de Mattos de Oliveira Coelho, Irene da Silva Barbosa, Helene Santos Souza, Miliane Moreira Soares de |
| author_role |
author |
| author2 |
Motta, Cássia Couto da Soares, Bianca da Silva Melo, Dayanne Araújo de Coelho, Shana de Mattos de Oliveira Coelho, Irene da Silva Barbosa, Helene Santos Souza, Miliane Moreira Soares de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marques, Viviane Figueira Motta, Cássia Couto da Soares, Bianca da Silva Melo, Dayanne Araújo de Coelho, Shana de Mattos de Oliveira Coelho, Irene da Silva Barbosa, Helene Santos Souza, Miliane Moreira Soares de |
| dc.subject.other.pt_BR.fl_str_mv |
Biofilmes Mastite Resistência antimicrobiana Staphylococcus aureus |
| topic |
Biofilmes Mastite Resistência antimicrobiana Staphylococcus aureus Biofilm Agr types Antimicrobial resistance Mastitis Staphylococcus aureus |
| dc.subject.en.pt_BR.fl_str_mv |
Biofilm Agr types Antimicrobial resistance Mastitis Staphylococcus aureus |
| description |
Universidade Federal Rural do Rio de Janeiro. Microbiologia e Imunologia Veterinária. Seropédica, RJ, Brasil. |
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2017 |
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2017-07-05T12:56:49Z |
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2017-07-05T12:56:49Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MARQUES, Viviane Figueira; et al. Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis. Brazilian Journal of Microbiology, v.48, n.1, p.118-124, Jan.- Mar. 2017. |
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https://www.arca.fiocruz.br/handle/icict/19719 |
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1517-8382 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.bjm.2016.10.001 |
| identifier_str_mv |
MARQUES, Viviane Figueira; et al. Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis. Brazilian Journal of Microbiology, v.48, n.1, p.118-124, Jan.- Mar. 2017. 1517-8382 10.1016/j.bjm.2016.10.001 |
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eng |
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Sociedade Brasileira de Microbiologia |
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Sociedade Brasileira de Microbiologia |
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