Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells

Detalhes bibliográficos
Autor(a) principal: Fonseca, Leonardo Marques da
Data de Publicação: 2020
Outros Autores: Silva, Lucas Rodrigues Jacques da, Reis, Jhenifer Santos dos, Santos, Marcos André Rodrigues da Costa, Chaves, Victoria de Sousa, Costa, Kelli Monteiro da, Diniz, Julliana de Nazareth Sá, Lima, Celio Geraldo Freire de, Morrot, Alexandre, Franklim, Tatiany Nunes, Pinto, Douglas Chaves de Alcântara, Lima, Marco Edilson Freire de, Previato, Jose Osvaldo, Previato, Lucia Mendonça, Lima, Leonardo Freire de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42523
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.
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spelling Fonseca, Leonardo Marques daSilva, Lucas Rodrigues Jacques daReis, Jhenifer Santos dosSantos, Marcos André Rodrigues da CostaChaves, Victoria de SousaCosta, Kelli Monteiro daDiniz, Julliana de Nazareth SáLima, Celio Geraldo Freire deMorrot, AlexandreFranklim, Tatiany NunesPinto, Douglas Chaves de AlcântaraLima, Marco Edilson Freire dePreviato, Jose OsvaldoPreviato, Lucia MendonçaLima, Leonardo Freire de2020-08-04T14:32:06Z2020-08-04T14:32:06Z2020FONSECA, Leonardo Marques da et al. Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells. Medicines, v. 7, n. 19, 16p, 2020.2305-6320https://www.arca.fiocruz.br/handle/icict/4252310.3390/medicines7040019engMDPIPiprerinaVias de sinalização de TGF-βEMTPulmão humanoCélulas de adenocarcinomaPiperineGF-β Signaling PathwaysEMTHuman LungAdenocarcinoma CellsPiperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Química. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Química. Seropédica, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Química. Seropédica, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.Background: Piperine, an amide extracted from the Piper spices, exhibits strong anti-tumor properties. However, its effect on the epithelial-mesenchymal transition (EMT) process has never been investigated. Herein, we evaluate the toxic effect of piperine on lung adenocarcinoma (A549), breast adenocarcinoma (MDA-MB-231) and hepatocellular carcinoma (HepG2) cell lines, as well as its ability to inhibit EMT-related events induced by TGF-β1 treatment. Methods: The cell viability was investigated by MTT assay. Protein expression was evaluated by Western blot. Gene expression was monitored by real-time PCR. Zymography assay was employed to detect metalloproteinase (MMP) activity in conditioned media. Cell motility was assessed by the wound-healing and phagokinetic gold sol assays. Results: The results revealed that piperine was cytotoxic in concentrations over 100 µM, showing IC50 values for HepG2, MDA-MB-231 and A549 cell lines of 214, 238 and 198 µM, respectively. In order to investigate whether piperine would reverse the TGF-β1 induced-EMT, the A549 cell line was pretreated with sublethal concentrations of the natural amide followed by the addition of TGF-β1. Besides disrupting EMT-related events, piperine also inhibited both ERK 1/2 and SMAD 2 phosphorylation. Conclusions: These results suggest that piperine might be further used in therapeutic strategies for metastatic cancer and EMT-related disorders.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42523/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlexandre Morrot_etal_IOC_2020.pdfAlexandre Morrot_etal_IOC_2020.pdfapplication/pdf2131728https://www.arca.fiocruz.br/bitstream/icict/42523/2/Alexandre%20Morrot_etal_IOC_2020.pdfe0d6a86050745ee9d61908e67b0f8d4eMD52TEXTAlexandre Morrot_etal_IOC_2020.pdf.txtAlexandre Morrot_etal_IOC_2020.pdf.txtExtracted texttext/plain73180https://www.arca.fiocruz.br/bitstream/icict/42523/3/Alexandre%20Morrot_etal_IOC_2020.pdf.txtf530c1081c69e0ef1db55810b313a6eaMD53icict/425232020-08-05 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dc.title.pt_BR.fl_str_mv Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
title Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
spellingShingle Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
Fonseca, Leonardo Marques da
Piprerina
Vias de sinalização de TGF-β
EMT
Pulmão humano
Células de adenocarcinoma
Piperine
GF-β Signaling Pathways
EMT
Human Lung
Adenocarcinoma Cells
title_short Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
title_full Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
title_fullStr Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
title_full_unstemmed Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
title_sort Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells
author Fonseca, Leonardo Marques da
author_facet Fonseca, Leonardo Marques da
Silva, Lucas Rodrigues Jacques da
Reis, Jhenifer Santos dos
Santos, Marcos André Rodrigues da Costa
Chaves, Victoria de Sousa
Costa, Kelli Monteiro da
Diniz, Julliana de Nazareth Sá
Lima, Celio Geraldo Freire de
Morrot, Alexandre
Franklim, Tatiany Nunes
Pinto, Douglas Chaves de Alcântara
Lima, Marco Edilson Freire de
Previato, Jose Osvaldo
Previato, Lucia Mendonça
Lima, Leonardo Freire de
author_role author
author2 Silva, Lucas Rodrigues Jacques da
Reis, Jhenifer Santos dos
Santos, Marcos André Rodrigues da Costa
Chaves, Victoria de Sousa
Costa, Kelli Monteiro da
Diniz, Julliana de Nazareth Sá
Lima, Celio Geraldo Freire de
Morrot, Alexandre
Franklim, Tatiany Nunes
Pinto, Douglas Chaves de Alcântara
Lima, Marco Edilson Freire de
Previato, Jose Osvaldo
Previato, Lucia Mendonça
Lima, Leonardo Freire de
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Leonardo Marques da
Silva, Lucas Rodrigues Jacques da
Reis, Jhenifer Santos dos
Santos, Marcos André Rodrigues da Costa
Chaves, Victoria de Sousa
Costa, Kelli Monteiro da
Diniz, Julliana de Nazareth Sá
Lima, Celio Geraldo Freire de
Morrot, Alexandre
Franklim, Tatiany Nunes
Pinto, Douglas Chaves de Alcântara
Lima, Marco Edilson Freire de
Previato, Jose Osvaldo
Previato, Lucia Mendonça
Lima, Leonardo Freire de
dc.subject.other.pt_BR.fl_str_mv Piprerina
Vias de sinalização de TGF-β
EMT
Pulmão humano
Células de adenocarcinoma
topic Piprerina
Vias de sinalização de TGF-β
EMT
Pulmão humano
Células de adenocarcinoma
Piperine
GF-β Signaling Pathways
EMT
Human Lung
Adenocarcinoma Cells
dc.subject.en.pt_BR.fl_str_mv Piperine
GF-β Signaling Pathways
EMT
Human Lung
Adenocarcinoma Cells
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Glicobiologia. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-04T14:32:06Z
dc.date.available.fl_str_mv 2020-08-04T14:32:06Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FONSECA, Leonardo Marques da et al. Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells. Medicines, v. 7, n. 19, 16p, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42523
dc.identifier.issn.pt_BR.fl_str_mv 2305-6320
dc.identifier.doi.none.fl_str_mv 10.3390/medicines7040019
identifier_str_mv FONSECA, Leonardo Marques da et al. Piperine Inhibits TGF-β Signaling Pathways and Disrupts EMT-Related Events in Human Lung Adenocarcinoma Cells. Medicines, v. 7, n. 19, 16p, 2020.
2305-6320
10.3390/medicines7040019
url https://www.arca.fiocruz.br/handle/icict/42523
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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