Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case

Detalhes bibliográficos
Autor(a) principal: Dario, Maria Augusta
Data de Publicação: 2022
Outros Autores: Lisboa, Cristiane Varella, Xavier, Samanta Cristina das Chagas, D`Andrea, Paulo Sérgio, Roque, André Luiz Rodrigues, Jansen, Ana Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53925
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
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spelling Dario, Maria AugustaLisboa, Cristiane VarellaXavier, Samanta Cristina das ChagasD`Andrea, Paulo SérgioRoque, André Luiz RodriguesJansen, Ana Maria2022-07-23T12:14:32Z2022-07-23T12:14:32Z2022DARIO, Maria Augusta et al. Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case. Frontiers in Cellular and Infection Microbiology, v. 12, Article 812708, p. 1 - 12, Feb. 2022.2235-2988https://www.arca.fiocruz.br/handle/icict/5392510.3389/fcimb.2022.812708engFrontiers MediaTrypanosomatidaeHospedeiro mamíferoClado Trypanosoma cruziInfecçãoMata AtlânticaTrypanosomatidaeMammalian hostTrypanosoma cruzi cladeInfectionAtlantic ForestTrypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Caseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.Trypanosomatids are hemoflagellate parasites that even though they have been increasingly studied, many aspects of their biology and taxonomy remain unknown. The aimof this study was to investigate the Trypanosoma sp. transmission cycle in nonflying small mammals in an area where a case of acute Chagas disease occurred in Mangaratibamunicipality, Rio de Janeiro state. Three expeditions were conducted in the area: the first in 2012, soon after the human case, and two others in 2015. Sylvatic mammals were captured and submitted to blood collection for trypanosomatid parasitological and serological exams. Dogs from the surrounding areas where the sylvatic mammals were captured were also tested for T. cruzi infection. DNA samples were extracted from blood clots and positive hemocultures, submitted to polymerase chain reaction targeting SSU rDNA and gGAPDH genes, sequenced and phylogenetic analysed. Twenty-one wild mammals were captured in 2012, mainly rodents, and 17 mammals, mainly marsupials, were captured in the two expeditions conducted in 2015. Only four rodents demonstrated borderline serological T. cruzi test (IFAT), two in 2012 and two in 2015. Trypanosoma janseni was the main Trypanosoma species identified, and isolates were obtained solely from Didelphis aurita. In addition to biological differences, molecular differences are suggestive of genetic diversity in this flagellate species. Trypanosoma sp. DID was identified in blood clots from D. aurita in single and mixed infections with T. janseni. Concerning dogs, 12 presented mostly borderline serological titers for T. cruzi and no positive hemoculture. In blood clots from 11 dogs, T. cruzi DNA was detected and characterized as TcI (n = 9) or TcII (n = 2). Infections by Trypanosoma rangeli lineage E (n = 2) and, for the first time, Trypanosoma caninum, Trypanosoma dionisii, and Crithidia mellificae (n = 1 each) were also detected in dogs. We concluded that despite the low mammalian species richness and degraded environment, a high Trypanosoma species richness species was being transmitted with the predominance of T. janseni and not T. cruzi, as would be expected in a locality of an acute case of Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAnamariaJansen_SamantaChagasXavier_etal_IOC_2022.pdfAnamariaJansen_SamantaChagasXavier_etal_IOC_2022.pdfapplication/pdf2633419https://www.arca.fiocruz.br/bitstream/icict/53925/2/AnamariaJansen_SamantaChagasXavier_etal_IOC_2022.pdf9b5372fc52cd6239beba623ae30a1b3bMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/53925/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/539252022-07-23 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dc.title.pt_BR.fl_str_mv Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
title Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
spellingShingle Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
Dario, Maria Augusta
Trypanosomatidae
Hospedeiro mamífero
Clado Trypanosoma cruzi
Infecção
Mata Atlântica
Trypanosomatidae
Mammalian host
Trypanosoma cruzi clade
Infection
Atlantic Forest
title_short Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
title_full Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
title_fullStr Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
title_full_unstemmed Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
title_sort Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case
author Dario, Maria Augusta
author_facet Dario, Maria Augusta
Lisboa, Cristiane Varella
Xavier, Samanta Cristina das Chagas
D`Andrea, Paulo Sérgio
Roque, André Luiz Rodrigues
Jansen, Ana Maria
author_role author
author2 Lisboa, Cristiane Varella
Xavier, Samanta Cristina das Chagas
D`Andrea, Paulo Sérgio
Roque, André Luiz Rodrigues
Jansen, Ana Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dario, Maria Augusta
Lisboa, Cristiane Varella
Xavier, Samanta Cristina das Chagas
D`Andrea, Paulo Sérgio
Roque, André Luiz Rodrigues
Jansen, Ana Maria
dc.subject.other.pt_BR.fl_str_mv Trypanosomatidae
Hospedeiro mamífero
Clado Trypanosoma cruzi
Infecção
Mata Atlântica
topic Trypanosomatidae
Hospedeiro mamífero
Clado Trypanosoma cruzi
Infecção
Mata Atlântica
Trypanosomatidae
Mammalian host
Trypanosoma cruzi clade
Infection
Atlantic Forest
dc.subject.en.pt_BR.fl_str_mv Trypanosomatidae
Mammalian host
Trypanosoma cruzi clade
Infection
Atlantic Forest
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-07-23T12:14:32Z
dc.date.available.fl_str_mv 2022-07-23T12:14:32Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DARIO, Maria Augusta et al. Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case. Frontiers in Cellular and Infection Microbiology, v. 12, Article 812708, p. 1 - 12, Feb. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53925
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2022.812708
identifier_str_mv DARIO, Maria Augusta et al. Trypanosoma Species in Small Nonflying Mammals in an Area With a Single Previous Chagas Disease Case. Frontiers in Cellular and Infection Microbiology, v. 12, Article 812708, p. 1 - 12, Feb. 2022.
2235-2988
10.3389/fcimb.2022.812708
url https://www.arca.fiocruz.br/handle/icict/53925
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/53925/2/AnamariaJansen_SamantaChagasXavier_etal_IOC_2022.pdf
https://www.arca.fiocruz.br/bitstream/icict/53925/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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