BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties

Detalhes bibliográficos
Autor(a) principal: Kayano, Anderson M.
Data de Publicação: 2015
Outros Autores: Simões-Silva, Rodrigo, Medeiros, Patrícia S. M., Maltarollo, Vinícius G., Honorio, Kathia M., Oliveira, Eliandre, Albericio, Fernando, Silva, Saulo L. da, Aguiar, Anna Caroline Campos, Krettli, Antoniana U., Fernandes, Carla F.C., Zuliani, Juliana P., Calderon, Leonardo A., Stábeli, Rodrigo G., Soares, Andreimar M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12539
Resumo: 2016-11-30
id CRUZ_16317dc9d2789f0b03459a4b75c91878
oai_identifier_str oai:www.arca.fiocruz.br:icict/12539
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Kayano, Anderson M.Simões-Silva, RodrigoMedeiros, Patrícia S. M.Maltarollo, Vinícius G.Honorio, Kathia M.Oliveira, EliandreAlbericio, FernandoSilva, Saulo L. daAguiar, Anna Caroline CamposKrettli, Antoniana U.Fernandes, Carla F.C.Zuliani, Juliana P.Calderon, Leonardo A.Stábeli, Rodrigo G.Soares, Andreimar M.2016-01-14T20:36:56Z2015KAYANA, Anderson M., et al. BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties. Toxicon, v. 106, p. 30-41, 2015.https://www.arca.fiocruz.br/handle/icict/1253910.1016/j.toxicon.2015.09.005engElsevierBbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial propertiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2016-11-30Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Universidade Federal do ABC. Centro de Ciências Naturais e Humanas. Santo André, SP, Brazil. / Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Farmácia. São Paulo, SP, Brazil.Universidade Federal do ABC. Centro de Ciências Naturais e Humanas. Santo André, SP, Brazil. / Universidade de São Paulo. Escola de Artes, Ciências e Humanidades. São Paulo, SP, Brazil.Barcelona Science Park. Proteomic Platform. Barcelona, Spain.Institute for Research in Biomedicine. Barcelona, Spain. / CIBER-BBN. Barcelona Science Park. Barcelona, Spain. / University of KwaZulu-Natal. School of Chemistry & Physics. Durban, South Africa.Universidade Federal de São João Del Rei. Departamento de Química, Biotecnologia e Engenharia de Bioprocessos. Ouro Branco, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Programa de Pós-Graduação em Biologia Experimental. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.This study describes the biochemical and functional characterization of a new metalloproteinase named BbMP-1, isolated from Bothrops brazili venom. BbMP-1 was homogeneous on SDS-PAGE, presented molecular mass of 22,933Da and pI 6.4. The primary structure was partially elucidated with high identity with others metalloproteinases from Viperidae venoms. The enzymatic activity on azocasein was evaluated in different experimental conditions (pH, temperature). A signicant reduction in enzyme activity after exposure to chelators of divalent cations (EDTA), reducing agents (DTT), pH less than 5.0 or temperatures higher than 45C was observed. BbMP-1 showed activity on brinogen degrading Aa chain quickly and to a lesser extent the Bb chain. Also demostrated to be weakly hemorrhagic, presenting however, signicant myotoxic and edematogenic activity. The in vitro activity of BbMP-1 against Plasmodium falciparum showed an IC50 of 3.2 ± 2.0 mg/mL. This study may help to understand the pathophysiological effects induced by this group of toxin and their participation in the symptoms observed in cases of snake envenomation. Moreover, this result is representative for this group of proteins and shows the biotechnological potential of BbMP-1 by the demonstration of its antiplasmodial activity.Snake venomB. braziliMetalloproteinaseEnzymatic characterizationFunctional characterizationAntiplasmodial activityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12539/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBbMP-1, a new metalloproteinase.pdfBbMP-1, a new metalloproteinase.pdfapplication/pdf1163290https://www.arca.fiocruz.br/bitstream/icict/12539/2/BbMP-1%2c%20a%20new%20metalloproteinase.pdfbdb756358cb2cfde1d9ca5e3560460ecMD52TEXTBbMP-1, a new metalloproteinase.pdf.txtBbMP-1, a new metalloproteinase.pdf.txtExtracted texttext/plain68212https://www.arca.fiocruz.br/bitstream/icict/12539/3/BbMP-1%2c%20a%20new%20metalloproteinase.pdf.txt9879ae117d898e084ffe8e1c928c44dfMD53icict/125392021-03-24 16:32:19.368oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
title BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
spellingShingle BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
Kayano, Anderson M.
Snake venom
B. brazili
Metalloproteinase
Enzymatic characterization
Functional characterization
Antiplasmodial activity
title_short BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
title_full BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
title_fullStr BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
title_full_unstemmed BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
title_sort BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties
author Kayano, Anderson M.
author_facet Kayano, Anderson M.
Simões-Silva, Rodrigo
Medeiros, Patrícia S. M.
Maltarollo, Vinícius G.
Honorio, Kathia M.
Oliveira, Eliandre
Albericio, Fernando
Silva, Saulo L. da
Aguiar, Anna Caroline Campos
Krettli, Antoniana U.
Fernandes, Carla F.C.
Zuliani, Juliana P.
Calderon, Leonardo A.
Stábeli, Rodrigo G.
Soares, Andreimar M.
author_role author
author2 Simões-Silva, Rodrigo
Medeiros, Patrícia S. M.
Maltarollo, Vinícius G.
Honorio, Kathia M.
Oliveira, Eliandre
Albericio, Fernando
Silva, Saulo L. da
Aguiar, Anna Caroline Campos
Krettli, Antoniana U.
Fernandes, Carla F.C.
Zuliani, Juliana P.
Calderon, Leonardo A.
Stábeli, Rodrigo G.
Soares, Andreimar M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kayano, Anderson M.
Simões-Silva, Rodrigo
Medeiros, Patrícia S. M.
Maltarollo, Vinícius G.
Honorio, Kathia M.
Oliveira, Eliandre
Albericio, Fernando
Silva, Saulo L. da
Aguiar, Anna Caroline Campos
Krettli, Antoniana U.
Fernandes, Carla F.C.
Zuliani, Juliana P.
Calderon, Leonardo A.
Stábeli, Rodrigo G.
Soares, Andreimar M.
dc.subject.en.pt_BR.fl_str_mv Snake venom
B. brazili
Metalloproteinase
Enzymatic characterization
Functional characterization
Antiplasmodial activity
topic Snake venom
B. brazili
Metalloproteinase
Enzymatic characterization
Functional characterization
Antiplasmodial activity
description 2016-11-30
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-14T20:36:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KAYANA, Anderson M., et al. BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties. Toxicon, v. 106, p. 30-41, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12539
dc.identifier.doi.none.fl_str_mv 10.1016/j.toxicon.2015.09.005
identifier_str_mv KAYANA, Anderson M., et al. BbMP-1, a new metalloproteinase isolated from Bothrops brazili snake venom with in vitro antiplasmodial properties. Toxicon, v. 106, p. 30-41, 2015.
10.1016/j.toxicon.2015.09.005
url https://www.arca.fiocruz.br/handle/icict/12539
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12539/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/12539/2/BbMP-1%2c%20a%20new%20metalloproteinase.pdf
https://www.arca.fiocruz.br/bitstream/icict/12539/3/BbMP-1%2c%20a%20new%20metalloproteinase.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
bdb756358cb2cfde1d9ca5e3560460ec
9879ae117d898e084ffe8e1c928c44df
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324888051646464

Registros relacionados