Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Cantanhêde, Lilian Motta
Data de Publicação: 2021
Outros Autores: Mattos, Cristiane Batista, Cruz, Ana Karoline, Ikenohuchi, Yoda Janaina, Fernandes, Flavia Gonçalves, Medeiros, Enmanuella Helga Ratier Terceiro, Silva Júnior, Cipriano Ferreira da, Cupolillo, Elisa, Ferreira, Gabriel Eduardo Melim, Ferreira, Ricardo de Godoi Mattos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50903
Resumo: Fundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.
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spelling Cantanhêde, Lilian MottaMattos, Cristiane BatistaCruz, Ana KarolineIkenohuchi, Yoda JanainaFernandes, Flavia GonçalvesMedeiros, Enmanuella Helga Ratier TerceiroSilva Júnior, Cipriano Ferreira daCupolillo, ElisaFerreira, Gabriel Eduardo MelimFerreira, Ricardo de Godoi Mattos2022-01-26T14:22:18Z2022-01-26T14:22:18Z2021CANTHANHÊDE, Lilian Motta et al. Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis. Pathogens, v. 10, 1116, p. 1 - 16, Sept. 2021.2076-0817https://www.arca.fiocruz.br/handle/icict/5090310.3390/ pathogens10091116engMDPILeishmaniose TegumentarLeishmaniose mucosaDiagnóstico molecularTegumentary leishmaniasisMucosal leishmaniasisMolecular diagnosisOvercoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilFundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, BrasilThe northern region of Brazil, which has the largest number of cases of tegumentary leishmaniasis (TL) in the country, is also the region that has the highest diversity of species of vectors and Leishmania parasites. In this region, cases of mucosal leishmaniasis (ML), a clinical form of TL, exceed the national average of cases, reaching up to 12% of the total annual TL notifications. ML is associated with multiple factors, such as the parasite species and the viral endosymbiont Leishmania RNA virus 1 (LRV1). Being a chronic parasitological disease, laboratory diagnosis of ML poses a challenge for health services. Here, we evaluated more than 700 clinical samples from patients with clinical suspicion of TL, including patients with cutaneous leishmaniasis (CL) and mucosal leishmaniasis, comparing the results of parasitological tests—direct parasitological examination by microscopy (DP) and conventional PCR (cPCR) targeting of both kDNA and hsp70. The DP was performed by collecting material from lesions through biopsies (mucosal lesions) or scarification (cutaneous lesions); for PCR, a cervical brush was used for sample collection. Blood samples were tested employing standardized real-time PCR (qPCR) protocol targeting the HSP70 gene. PCR tests showed higher sensitivity than DP for both CL and ML samples. Considering ML samples only (N = 89), DP showed a sensitivity of 49.4% (N = 44) against 98.8% (N = 88) for kDNA PCR. The qPCR hsp70 for blood samples from patients with ML (N = 14) resulted in superior sensitivity (50%; N = 7) compared to DP (21.4%; N = 3) for samples from the same patients. Our results reinforced the need to implement a molecular test for the diagnosis of ML, in addition to proposing methods less invasive for collecting material from TL patients. Sample collection using a cervical brush in lesions observed in CL and ML patients is easy to perform and less invasive, compared to scarification and biopsies. Blood samples could be a good source for qPCR diagnosis for ML patients. Thus, we propose here a standardized method for collection and for performing of molecular diagnosis of clinical samples from suspicious ML patients that can be applied in reference services for improving ML diagnosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50903/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLilianMCatanhede_ElisaCupolillo_etal_IOC_2021.pdfLilianMCatanhede_ElisaCupolillo_etal_IOC_2021.pdfapplication/pdf828187https://www.arca.fiocruz.br/bitstream/icict/50903/2/LilianMCatanhede_ElisaCupolillo_etal_IOC_2021.pdf2db8bcc16aa4a8736d898e69a6f8b825MD52icict/509032022-01-26 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dc.title.pt_BR.fl_str_mv Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
title Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
spellingShingle Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
Cantanhêde, Lilian Motta
Leishmaniose Tegumentar
Leishmaniose mucosa
Diagnóstico molecular
Tegumentary leishmaniasis
Mucosal leishmaniasis
Molecular diagnosis
title_short Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
title_full Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
title_fullStr Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
title_full_unstemmed Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
title_sort Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis
author Cantanhêde, Lilian Motta
author_facet Cantanhêde, Lilian Motta
Mattos, Cristiane Batista
Cruz, Ana Karoline
Ikenohuchi, Yoda Janaina
Fernandes, Flavia Gonçalves
Medeiros, Enmanuella Helga Ratier Terceiro
Silva Júnior, Cipriano Ferreira da
Cupolillo, Elisa
Ferreira, Gabriel Eduardo Melim
Ferreira, Ricardo de Godoi Mattos
author_role author
author2 Mattos, Cristiane Batista
Cruz, Ana Karoline
Ikenohuchi, Yoda Janaina
Fernandes, Flavia Gonçalves
Medeiros, Enmanuella Helga Ratier Terceiro
Silva Júnior, Cipriano Ferreira da
Cupolillo, Elisa
Ferreira, Gabriel Eduardo Melim
Ferreira, Ricardo de Godoi Mattos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cantanhêde, Lilian Motta
Mattos, Cristiane Batista
Cruz, Ana Karoline
Ikenohuchi, Yoda Janaina
Fernandes, Flavia Gonçalves
Medeiros, Enmanuella Helga Ratier Terceiro
Silva Júnior, Cipriano Ferreira da
Cupolillo, Elisa
Ferreira, Gabriel Eduardo Melim
Ferreira, Ricardo de Godoi Mattos
dc.subject.other.pt_BR.fl_str_mv Leishmaniose Tegumentar
Leishmaniose mucosa
Diagnóstico molecular
topic Leishmaniose Tegumentar
Leishmaniose mucosa
Diagnóstico molecular
Tegumentary leishmaniasis
Mucosal leishmaniasis
Molecular diagnosis
dc.subject.en.pt_BR.fl_str_mv Tegumentary leishmaniasis
Mucosal leishmaniasis
Molecular diagnosis
description Fundação Oswaldo Cruz. Laboratório de Epidemiologia Genética. Porto Velho, RO, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmanioses. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-26T14:22:18Z
dc.date.available.fl_str_mv 2022-01-26T14:22:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CANTHANHÊDE, Lilian Motta et al. Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis. Pathogens, v. 10, 1116, p. 1 - 16, Sept. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50903
dc.identifier.issn.pt_BR.fl_str_mv 2076-0817
dc.identifier.doi.none.fl_str_mv 10.3390/ pathogens10091116
identifier_str_mv CANTHANHÊDE, Lilian Motta et al. Overcoming the Negligence in Laboratory Diagnosis of Mucosal Leishmaniasis. Pathogens, v. 10, 1116, p. 1 - 16, Sept. 2021.
2076-0817
10.3390/ pathogens10091116
url https://www.arca.fiocruz.br/handle/icict/50903
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50903/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50903/2/LilianMCatanhede_ElisaCupolillo_etal_IOC_2021.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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