Revisitingmolecular serotyping of Streptococcus pneumonia
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14922 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brasil / Fundação Ezequiel Dias. Serviço de Bactérias e Doenças de Fungos. Belo Horizonte, MG, Brasil. |
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Camargo, Dhian Renato AlmeidaPais, Fabiano Sviatopolk MirskyVolpini, Ângela CristinaOliveira, Marluce AACoimbra, Roney Santos2016-07-06T11:43:26Z2016-07-14T19:10:31Z2016-07-06T11:43:26Z2016-07-14T19:10:31Z2015CAMARGO, Dhian Renato Almeida et al. Revisitingmolecular serotyping of Streptococcus pneumonia. BMC Genomics. v. 16, Suppl 5, p. 1-10, 2015.1471-2164https://www.arca.fiocruz.br/handle/icict/1492210.1186/1471-2164-16-S5-S1engBiomed CentralRevisitingmolecular serotyping of Streptococcus pneumoniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brasil / Fundação Ezequiel Dias. Serviço de Bactérias e Doenças de Fungos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Grupo de Biologia e Genômica Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Serviço de Bactérias e Doenças de Fungos. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brasil.Background: Ninety-two Streptococcus pneumoniae serotypes have been described so far, but the pneumococcal conjugate vaccine introduced in the Brazilian basic vaccination schedule in 2010 covers only the ten most prevalent in the country. Pneumococcal serotype-shifting after massive immunization is a major concern and monitoring this phenomenon requires efficient and accessible serotyping methods. Pneumococcal serotyping based on antisera produced in animals is laborious and restricted to a few reference laboratories. Alternatively, molecular serotyping methods assess polymorphisms in the cps gene cluster, which encodes key enzymes for capsular polysaccharides synthesis in pneumococci. In one such approach, cps-RFLP, the PCR amplified cps loci are digested with an endonuclease, generating serotype-specific fingerprints on agarose gel electrophoresis. Methods: In this work, in silico and in vitro approaches were combined to demonstrate that XhoII is the most discriminating endonuclease for cps-RFLP, and to build a database of serotype-specific fingerprints that accommodates the genetic diversity within the cps locus of 92 known pneumococci serotypes. Results: The expected specificity of cps-RFLP using XhoII was 76% for serotyping and 100% for serogrouping. The database of cps-RFLP fingerprints was integrated to Molecular Serotyping Tool (MST), a previously published web-based software for molecular serotyping. In addition, 43 isolates representing 29 serotypes prevalent in the state of Minas Gerais, Brazil, from 2007 to 2013, were examined in vitro; 11 serotypes (nine serogroups) matched the respective in silico patterns calculated for reference strains. The remaining experimental patterns, despite their resemblance to their expected in silico patterns, did not reach the threshold of similarity score to be considered a match and were then added to the database. Conclusion: The cps-RFLP method with XhoII outperformed the antisera-based and other molecular serotyping methods in regard of the expected specificity. In order to accommodate the genetic variability of the pneumococci cps loci, the database of cps-RFLP patterns will be progressively expanded to include new variant in vitro patterns. The cps-RFLP method with endonuclease XhoII coupled with MST for computer-assisted interpretation of results may represent a relevant contribution to the real time detection of changes in regional pneumococci population diversity in response to mass immunization programs.Streptococcus pneumoniaecps gene clusterMolecular serotypingTyping methodscps-RFLPBioinformaticsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTve_Dhian_Camargo_ Revisitingmolecular_CPqRR_2015.pdf.txtve_Dhian_Camargo_ Revisitingmolecular_CPqRR_2015.pdf.txtExtracted texttext/plain41150https://www.arca.fiocruz.br/bitstream/icict/14922/4/ve_Dhian_Camargo_%20Revisitingmolecular_CPqRR_2015.pdf.txt677ee69411513ac8a87f484354a4dac8MD54ORIGINALve_Dhian_Camargo_ Revisitingmolecular_CPqRR_2015.pdfapplication/pdf1647355https://www.arca.fiocruz.br/bitstream/icict/14922/2/ve_Dhian_Camargo_%20Revisitingmolecular_CPqRR_2015.pdfd446af4ae8ae1cdd1b0e0853582ff5ecMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14922/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/149222019-09-26 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dc.title.pt_BR.fl_str_mv |
Revisitingmolecular serotyping of Streptococcus pneumonia |
title |
Revisitingmolecular serotyping of Streptococcus pneumonia |
spellingShingle |
Revisitingmolecular serotyping of Streptococcus pneumonia Camargo, Dhian Renato Almeida Streptococcus pneumoniae cps gene cluster Molecular serotyping Typing methods cps-RFLP Bioinformatics |
title_short |
Revisitingmolecular serotyping of Streptococcus pneumonia |
title_full |
Revisitingmolecular serotyping of Streptococcus pneumonia |
title_fullStr |
Revisitingmolecular serotyping of Streptococcus pneumonia |
title_full_unstemmed |
Revisitingmolecular serotyping of Streptococcus pneumonia |
title_sort |
Revisitingmolecular serotyping of Streptococcus pneumonia |
author |
Camargo, Dhian Renato Almeida |
author_facet |
Camargo, Dhian Renato Almeida Pais, Fabiano Sviatopolk Mirsky Volpini, Ângela Cristina Oliveira, Marluce AA Coimbra, Roney Santos |
author_role |
author |
author2 |
Pais, Fabiano Sviatopolk Mirsky Volpini, Ângela Cristina Oliveira, Marluce AA Coimbra, Roney Santos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Camargo, Dhian Renato Almeida Pais, Fabiano Sviatopolk Mirsky Volpini, Ângela Cristina Oliveira, Marluce AA Coimbra, Roney Santos |
dc.subject.en.pt_BR.fl_str_mv |
Streptococcus pneumoniae cps gene cluster Molecular serotyping Typing methods cps-RFLP Bioinformatics |
topic |
Streptococcus pneumoniae cps gene cluster Molecular serotyping Typing methods cps-RFLP Bioinformatics |
description |
Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brasil / Fundação Ezequiel Dias. Serviço de Bactérias e Doenças de Fungos. Belo Horizonte, MG, Brasil. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-07-06T11:43:26Z 2016-07-14T19:10:31Z |
dc.date.available.fl_str_mv |
2016-07-06T11:43:26Z 2016-07-14T19:10:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CAMARGO, Dhian Renato Almeida et al. Revisitingmolecular serotyping of Streptococcus pneumonia. BMC Genomics. v. 16, Suppl 5, p. 1-10, 2015. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/14922 |
dc.identifier.issn.pt_BR.fl_str_mv |
1471-2164 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/1471-2164-16-S5-S1 |
identifier_str_mv |
CAMARGO, Dhian Renato Almeida et al. Revisitingmolecular serotyping of Streptococcus pneumonia. BMC Genomics. v. 16, Suppl 5, p. 1-10, 2015. 1471-2164 10.1186/1471-2164-16-S5-S1 |
url |
https://www.arca.fiocruz.br/handle/icict/14922 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Biomed Central |
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Biomed Central |
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