HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil

Detalhes bibliográficos
Autor(a) principal: Spano, Liliana Cruz
Data de Publicação: 2007
Outros Autores: Ferreira, Mônica Simões Rocha, Almeida, Marilda Santos, Nascimento, Jussara Pereira do, Leite, José Paulo Gagliardi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S1517-83822007000300008
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36628
Resumo: Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia e Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
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spelling Spano, Liliana CruzFerreira, Mônica Simões RochaAlmeida, Marilda SantosNascimento, Jussara Pereira doLeite, José Paulo Gagliardi2019-10-22T13:16:50Z2019-10-22T13:16:50Z2007SPANO, Liliana Cruz et al. HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil. Brazilian Journal of Microbiology, São Paulo, v. 38, n. 3, p. 424-429, July/Sept. 2007.1517-8382https://www.arca.fiocruz.br/handle/icict/3662810.1590/S1517-838220070003000081678-4405engUniversidade de São Paulo. Sociedade Brasileira de MicrobiologiaCitomegalovírusSecreção cervicalTecidos da placentagB GenótiposPolimorfismo do comprimento do fragmento de restriçãoCytomegalovirusRFLPgB genotypesCervical secretionPlacenta tissuesHCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn BrazilGenótipos gB de citomegalovírus humano em secreção cervical e placenta no Espírito Santo, Sudeste do Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia e Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Centro Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Human cytomegalovirus (HCMV) displays genetic variability in several regions, supposed to be related with strain-specific tissue tropism and immunopathogenesis. Based on sequence variation in the UL55 gene that encodes gB glycoprotein, HCMV strains can be assigned to one of four genotypes. Previous studies have addressed gB genotyping mostly by investigating strains derived from immunosuppressed patients, sometimes without previous knowledge about genotype distribution in a geographic area. The present study verified the distribution of HCMV gB genotypes of strains obtained from immunocompetent women at Vitória City, Espírito Santo State, Southeastern, Brazil. The HCMV genome was extracted from their cervical secretion, fetal and maternal placenta tissues (chorionic villous and decidua) from abortion cases and from white blood cells (WBCs). HCMV genotyping was performed by restriction fragment length polymorphism analyses of amplified product from the high variability site of the UL55 gene. All four genotypes were observed in both cervical secretion and placenta, whereas in WBCs a single gB1 genotype was detected. HCMV gB1 and gB2 genotypes were detected, respectively, in nine and in six of the 23 studied samples, while gB3 and gB4 were each found in four separate samples of the total. The differences in genotype frequency were not considered statistically significant. No mixed genotype infection was observed. The results indicated that the four gB HCMV genotypes had no particular tropism for placenta tissues and that all genotypes circulated within immunocompetent women at the time and in the region of study.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36628/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLilianaCSpano_JosePauloGLeite_etal_IOC_2007.pdfLilianaCSpano_JosePauloGLeite_etal_IOC_2007.pdfapplication/pdf126030https://www.arca.fiocruz.br/bitstream/icict/36628/2/LilianaCSpano_JosePauloGLeite_etal_IOC_2007.pdf2956a04469c21ad640c5685e03ad95e7MD52TEXTLilianaCSpano_JosePauloGLeite_etal_IOC_2007.pdf.txtLilianaCSpano_JosePauloGLeite_etal_IOC_2007.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
dc.title.alternative.pt_BR.fl_str_mv Genótipos gB de citomegalovírus humano em secreção cervical e placenta no Espírito Santo, Sudeste do Brasil
title HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
spellingShingle HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
Spano, Liliana Cruz
Citomegalovírus
Secreção cervical
Tecidos da placenta
gB Genótipos
Polimorfismo do comprimento do fragmento de restrição
Cytomegalovirus
RFLP
gB genotypes
Cervical secretion
Placenta tissues
title_short HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
title_full HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
title_fullStr HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
title_full_unstemmed HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
title_sort HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil
author Spano, Liliana Cruz
author_facet Spano, Liliana Cruz
Ferreira, Mônica Simões Rocha
Almeida, Marilda Santos
Nascimento, Jussara Pereira do
Leite, José Paulo Gagliardi
author_role author
author2 Ferreira, Mônica Simões Rocha
Almeida, Marilda Santos
Nascimento, Jussara Pereira do
Leite, José Paulo Gagliardi
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Spano, Liliana Cruz
Ferreira, Mônica Simões Rocha
Almeida, Marilda Santos
Nascimento, Jussara Pereira do
Leite, José Paulo Gagliardi
dc.subject.other.pt_BR.fl_str_mv Citomegalovírus
Secreção cervical
Tecidos da placenta
gB Genótipos
Polimorfismo do comprimento do fragmento de restrição
topic Citomegalovírus
Secreção cervical
Tecidos da placenta
gB Genótipos
Polimorfismo do comprimento do fragmento de restrição
Cytomegalovirus
RFLP
gB genotypes
Cervical secretion
Placenta tissues
dc.subject.en.pt_BR.fl_str_mv Cytomegalovirus
RFLP
gB genotypes
Cervical secretion
Placenta tissues
description Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia e Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2019-10-22T13:16:50Z
dc.date.available.fl_str_mv 2019-10-22T13:16:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SPANO, Liliana Cruz et al. HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil. Brazilian Journal of Microbiology, São Paulo, v. 38, n. 3, p. 424-429, July/Sept. 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36628
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.doi.none.fl_str_mv 10.1590/S1517-83822007000300008
dc.identifier.eissn.none.fl_str_mv 1678-4405
identifier_str_mv SPANO, Liliana Cruz et al. HCMV gB genotypes in cervical secretion and placenta tissues in the state of Espírito Santo, Southeastearn Brazil. Brazilian Journal of Microbiology, São Paulo, v. 38, n. 3, p. 424-429, July/Sept. 2007.
1517-8382
10.1590/S1517-83822007000300008
1678-4405
url https://www.arca.fiocruz.br/handle/icict/36628
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de São Paulo. Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Universidade de São Paulo. Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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