Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Morais, Mariana Abrahão Bueno de
Data de Publicação: 2013
Outros Autores: Souza, Tatiana de Arruda Campos Brasil de, Murakami, Mario Tyago
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15600
Resumo: This research was supported by grants from Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
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spelling Morais, Mariana Abrahão Bueno deSouza, Tatiana de Arruda Campos Brasil deMurakami, Mario Tyago2016-09-02T17:57:41Z2016-09-02T17:57:41Z2013MORAIS, Mariana Abrahão Bueno de et al. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis. Acta Crystallogr. Sect F. Struct. Biol. Cryst. Commun, n. 69, p. 408-411.1744-3091https://www.arca.fiocruz.br/handle/icict/1560010.1107/S1744309113003989This research was supported by grants from Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil.Tryparedoxin peroxidase (TXNPx) is an essential constituent of the main enzymatic scavenger system for reactive oxygen species (ROS) in trypanosomatids. Genetic studies have demonstrated the importance of this system for the development and virulence of these parasites, representing a potential target for the discovery of new trypanocidal drugs. In this work, the mitochondrial TXNPx from Leishmania braziliensis was cloned, overexpressed, purified and crystallized. The crystals diffracted to 3.3 Å resolution and belonged to space group P4(2)2(1)2, with unit-cell parameters a = b = 131.8, c = 44.4 Å. These studies will contribute to a better understanding of the molecular mechanisms involved in ROS detoxification by trypanosomatids.porCloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmania braziliensistryparedoxin peroxidaseCrystallizationMacromolecular SubstancesCrystallographyCristalizaçãoSubstâncias MacromolecularesCristalografiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/15600/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALCloning, expression, purification, crystallization.pdfCloning, expression, purification, 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dc.title.pt_BR.fl_str_mv Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
title Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
spellingShingle Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
Morais, Mariana Abrahão Bueno de
Leishmania braziliensis
tryparedoxin peroxidase
Crystallization
Macromolecular Substances
Crystallography
Cristalização
Substâncias Macromoleculares
Cristalografia
title_short Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
title_full Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
title_fullStr Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
title_full_unstemmed Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
title_sort Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis
author Morais, Mariana Abrahão Bueno de
author_facet Morais, Mariana Abrahão Bueno de
Souza, Tatiana de Arruda Campos Brasil de
Murakami, Mario Tyago
author_role author
author2 Souza, Tatiana de Arruda Campos Brasil de
Murakami, Mario Tyago
author2_role author
author
dc.contributor.author.fl_str_mv Morais, Mariana Abrahão Bueno de
Souza, Tatiana de Arruda Campos Brasil de
Murakami, Mario Tyago
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
tryparedoxin peroxidase
topic Leishmania braziliensis
tryparedoxin peroxidase
Crystallization
Macromolecular Substances
Crystallography
Cristalização
Substâncias Macromoleculares
Cristalografia
dc.subject.decs.En.fl_str_mv Crystallization
Macromolecular Substances
Crystallography
dc.subject.decs.pt_BR.fl_str_mv Cristalização
Substâncias Macromoleculares
Cristalografia
description This research was supported by grants from Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-09-02T17:57:41Z
dc.date.available.fl_str_mv 2016-09-02T17:57:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAIS, Mariana Abrahão Bueno de et al. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis. Acta Crystallogr. Sect F. Struct. Biol. Cryst. Commun, n. 69, p. 408-411.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15600
dc.identifier.issn.pt_BR.fl_str_mv 1744-3091
dc.identifier.doi.pt_BR.fl_str_mv 10.1107/S1744309113003989
identifier_str_mv MORAIS, Mariana Abrahão Bueno de et al. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis. Acta Crystallogr. Sect F. Struct. Biol. Cryst. Commun, n. 69, p. 408-411.
1744-3091
10.1107/S1744309113003989
url https://www.arca.fiocruz.br/handle/icict/15600
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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