A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.microc.2022.107600 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/53371 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. |
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Mendonça, Geovani TorezinStracke, Mateus CassaboniCoelho, Bruna de OliveiraSanchuki, Heloisa Bruna SoligoOliveira, Viviane Klassen deMarchini, Fabricio KleryntonZanette, Dalila LucíolaAoki, Mateus NóbregaViana, Emilson RibeiroBlanes, Lucas2022-06-20T15:24:26Z2022-06-20T15:24:26Z2022MENDONÇA, Geovani Torezin et al. A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics. Microchemical Journal, v. 180, n. 107600, p. 1-9, Sept. 2022.0026-265Xhttps://www.arca.fiocruz.br/handle/icict/5337110.1016/j.microc.2022.1076001095-9149porElsevierSARS-CoV-2COVID-19DiagnósticoLAMP-on-a-chipSARS-CoV-2Raspberry PiCOVID-19DiagnosticOpenCVSARS-CoV-2COVID-19A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnosticsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Universidade Tecnológica Federal do Paraná. Departamento de Física. Laboratório de Pesquisa e Caracterização de Nanomateriais e Nanodipositivos. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.This work describes the development of a Point-of-Care (POC) Lab-on-a-Chip (LOC) instrument for diagnosis of SARS-CoV-2 by Reverse-Transcription Loop-mediated isothermal amplification (RT-LAMP). The hardware is based on a Raspberry Pi computer ($35), a video camera, an Arduino Nano microcontroller, a printed circuit board as a heater and a 3D printed housing. The chips were manufactured in polymethyl methacrylate (PMMA) using a CO2 laser cutting machine and sealed with a PCR optic plastic film. The chip temperature is precisely controlled by a proportional–integral–derivative (PID) algorithm.During the RT-LAMP amplifications the chip was maintained at ~ (65.0 ± 0.1) ◦C for 25 minutes and 5 minutes cooling down, totaling a 30 minutes of reaction .The software interpretation occurs in less than a second. The chip design has four 25 µL chambers, two for clinical samples and two for positive and negative control-samples. The RT-LAMP master mix solution added in the chip chambers contains the pH indicator Phenol Red, that is pink (for pH ~ 8.0) before amplification and becomes yellow (pH ~ 6.0) if the genetic material is amplified. The RT-LAMP SARS-CoV-2 diagnostic was made by color image recognition using the OpenCV machine vision software library. The software was programmed to automatically distinguish the HSV color parameter distribution in each one of the four chip chambers. The instrument was successfully tested for SARS-CoV-2 diagnosis, in 22 clinic samples, 11 positives and 11 negatives, achieving an assertiveness of 86% when compared to the results obtained by RT-LAMP standard reactions performed in conventional PCR equipment.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/53371/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1-s2.0-S0026265X22004283-ok.pdf1-s2.0-S0026265X22004283-ok.pdfapplication/pdf5499887https://www.arca.fiocruz.br/bitstream/icict/53371/2/1-s2.0-S0026265X22004283-ok.pdfc4332f1ffe9f358548da67f99dba37c7MD52icict/533712022-11-17 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| dc.title.pt_BR.fl_str_mv |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics |
| title |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics |
| spellingShingle |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics Mendonça, Geovani Torezin SARS-CoV-2 COVID-19 Diagnóstico LAMP-on-a-chip SARS-CoV-2 Raspberry Pi COVID-19 Diagnostic OpenCV SARS-CoV-2 COVID-19 |
| title_short |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics |
| title_full |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics |
| title_fullStr |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics |
| title_full_unstemmed |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics |
| title_sort |
A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics |
| author |
Mendonça, Geovani Torezin |
| author_facet |
Mendonça, Geovani Torezin Stracke, Mateus Cassaboni Coelho, Bruna de Oliveira Sanchuki, Heloisa Bruna Soligo Oliveira, Viviane Klassen de Marchini, Fabricio Klerynton Zanette, Dalila Lucíola Aoki, Mateus Nóbrega Viana, Emilson Ribeiro Blanes, Lucas |
| author_role |
author |
| author2 |
Stracke, Mateus Cassaboni Coelho, Bruna de Oliveira Sanchuki, Heloisa Bruna Soligo Oliveira, Viviane Klassen de Marchini, Fabricio Klerynton Zanette, Dalila Lucíola Aoki, Mateus Nóbrega Viana, Emilson Ribeiro Blanes, Lucas |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mendonça, Geovani Torezin Stracke, Mateus Cassaboni Coelho, Bruna de Oliveira Sanchuki, Heloisa Bruna Soligo Oliveira, Viviane Klassen de Marchini, Fabricio Klerynton Zanette, Dalila Lucíola Aoki, Mateus Nóbrega Viana, Emilson Ribeiro Blanes, Lucas |
| dc.subject.other.pt_BR.fl_str_mv |
SARS-CoV-2 COVID-19 Diagnóstico |
| topic |
SARS-CoV-2 COVID-19 Diagnóstico LAMP-on-a-chip SARS-CoV-2 Raspberry Pi COVID-19 Diagnostic OpenCV SARS-CoV-2 COVID-19 |
| dc.subject.en.en_US.fl_str_mv |
LAMP-on-a-chip SARS-CoV-2 Raspberry Pi COVID-19 Diagnostic OpenCV |
| dc.subject.decs.pt_BR.fl_str_mv |
SARS-CoV-2 COVID-19 |
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Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciencias e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. |
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2022 |
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2022-06-20T15:24:26Z |
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2022-06-20T15:24:26Z |
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2022 |
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MENDONÇA, Geovani Torezin et al. A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics. Microchemical Journal, v. 180, n. 107600, p. 1-9, Sept. 2022. |
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https://www.arca.fiocruz.br/handle/icict/53371 |
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0026-265X |
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10.1016/j.microc.2022.107600 |
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1095-9149 |
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MENDONÇA, Geovani Torezin et al. A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics. Microchemical Journal, v. 180, n. 107600, p. 1-9, Sept. 2022. 0026-265X 10.1016/j.microc.2022.107600 1095-9149 |
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Elsevier |
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