The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence

Detalhes bibliográficos
Autor(a) principal: Mittra, Bidyottam
Data de Publicação: 2017
Outros Autores: Silva, Maria Fernanda Laranjeira, Miguel, Danilo Ciccone, Menezes, Juliana Perrone Bezerra de, Andrews, Norma W
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1074/jbc.M116.772624
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25492
Resumo: Menezes, Juliana Perrone Bezerra de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Mittra, BidyottamSilva, Maria Fernanda LaranjeiraMiguel, Danilo CicconeMenezes, Juliana Perrone Bezerra deAndrews, Norma W2018-03-26T14:34:09Z2018-03-26T14:34:09Z2017MITTRA, B. et al. The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence. Journal of Biological Chemistry, v. 292, n. 29, p. 12324–12338, 2017.0021-9258https://www.arca.fiocruz.br/handle/icict/2549210.1074/jbc.M116.772624Menezes, Juliana Perrone Bezerra de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.National Institutes of Health Grant R01 AI067979 (to N. W. A.).University of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USAUniversity of Maryland. Department of Cell Biology and Molecular Genetics. Maryland, USALeishmaniasis is one of the leading globally neglected diseases, affecting millions of people worldwide.Leishmaniainfection depends on the ability of insect-transmitted metacyclic promastigotes to invade mammalian hosts, differentiate into amastigotes, and replicate inside macrophages. To counter the hostile oxidative environment inside macrophages, these protozoans contain anti-oxidant systems that include iron-dependent superoxide dismutases (SODs) in mitochondria and glycosomes. Increasing evidence suggests that in addition to this protective role,Leishmaniamitochondrial SOD may also initiate H2O2-mediated redox signaling that regulates gene expression and metabolic changes associated with differentiation into virulent forms. To investigate this hypothesis, we examined the specific role of SODA, the mitochondrial SOD isoform inLeishmania amazonensisOur inability to generateL. amazonensis SODAnull mutants and the lethal phenotype observed following RNAi-mediated silencing of theTrypanosoma brucei SODAortholog suggests that SODA is essential for trypanosomatid survival.L. amazonensismetacyclic promastigotes lacking oneSODAallele failed to replicate in macrophages and were severely attenuated in their ability to generate cutaneous lesions in mice. Reduced expression of SODA also resulted in mitochondrial oxidative damage and failure ofSODA/ΔsodApromastigotes to differentiate into axenic amastigotes. SODA expression above a critical threshold was also required for the development of metacyclic promastigotes, asSODA/ΔsodAcultures were strongly depleted in this infective form and more susceptible to reactive oxygen species (ROS)-induced stress. Collectively, our data suggest that SODA promotesLeishmaniavirulence by protecting the parasites against mitochondrion-generated oxidative stress and by initiating ROS-mediated signaling mechanisms required for the differentiation of infective forms.engAmerican Society for Biochemistry and Molecular BiologyLeishmaniaDiferenciaçãoFerro superóxido dismutaseMitocôndriaSinalização redoxSuperóxido dismutase (SODLeishmaniaDifferentiationIron superoxide dismutaseMitochondriaRedox signalingSuperoxide dismutase (SOD)The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25492/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMittra B The 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dc.title.pt_BR.fl_str_mv The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
title The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
spellingShingle The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
Mittra, Bidyottam
Leishmania
Diferenciação
Ferro superóxido dismutase
Mitocôndria
Sinalização redox
Superóxido dismutase (SOD
Leishmania
Differentiation
Iron superoxide dismutase
Mitochondria
Redox signaling
Superoxide dismutase (SOD)
title_short The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
title_full The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
title_fullStr The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
title_full_unstemmed The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
title_sort The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence
author Mittra, Bidyottam
author_facet Mittra, Bidyottam
Silva, Maria Fernanda Laranjeira
Miguel, Danilo Ciccone
Menezes, Juliana Perrone Bezerra de
Andrews, Norma W
author_role author
author2 Silva, Maria Fernanda Laranjeira
Miguel, Danilo Ciccone
Menezes, Juliana Perrone Bezerra de
Andrews, Norma W
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mittra, Bidyottam
Silva, Maria Fernanda Laranjeira
Miguel, Danilo Ciccone
Menezes, Juliana Perrone Bezerra de
Andrews, Norma W
dc.subject.other.pt_BR.fl_str_mv Leishmania
Diferenciação
Ferro superóxido dismutase
Mitocôndria
Sinalização redox
Superóxido dismutase (SOD
topic Leishmania
Diferenciação
Ferro superóxido dismutase
Mitocôndria
Sinalização redox
Superóxido dismutase (SOD
Leishmania
Differentiation
Iron superoxide dismutase
Mitochondria
Redox signaling
Superoxide dismutase (SOD)
dc.subject.en.pt_BR.fl_str_mv Leishmania
Differentiation
Iron superoxide dismutase
Mitochondria
Redox signaling
Superoxide dismutase (SOD)
description Menezes, Juliana Perrone Bezerra de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-03-26T14:34:09Z
dc.date.available.fl_str_mv 2018-03-26T14:34:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MITTRA, B. et al. The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence. Journal of Biological Chemistry, v. 292, n. 29, p. 12324–12338, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25492
dc.identifier.issn.pt_BR.fl_str_mv 0021-9258
dc.identifier.doi.none.fl_str_mv 10.1074/jbc.M116.772624
identifier_str_mv MITTRA, B. et al. The iron-dependent mitochondrial superoxide dismutase SODA promotesLeishmania virulence. Journal of Biological Chemistry, v. 292, n. 29, p. 12324–12338, 2017.
0021-9258
10.1074/jbc.M116.772624
url https://www.arca.fiocruz.br/handle/icict/25492
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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