Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli

Detalhes bibliográficos
Autor(a) principal: Romero, Ibeth
Data de Publicação: 2014
Outros Autores: Téllez, Jair, Yamanaka, Lais Eiko, Steindel, Mario, Romanha, Alvaro José, Grisard, Edmundo Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9479
Resumo: Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil.
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spelling Romero, IbethTéllez, JairYamanaka, Lais EikoSteindel, MarioRomanha, Alvaro JoséGrisard, Edmundo Carlos2015-02-10T17:26:26Z2015-02-10T17:26:26Z2014ROMERO, Ibeth et al. Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli. Parasites & Vectors, v. 7, n. 1, p. 197, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/9479engBioMed CentralTranssulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil.BACKGROUND: Cysteine, a sulfur-containing amino acid, plays an important role in a variety of cellular functions such as protein biosynthesis, methylation, and polyamine and glutathione syntheses. In trypanosomatids, glutathione is conjugated with spermidine to form the specific antioxidant thiol trypanothione (T[SH]2) that plays a central role in maintaining intracellular redox homeostasis and providing defence against oxidative stress. METHODS: We cloned and characterised genes coding for a cystathionine β-synthase (CβS) and cysteine synthase (CS), key enzymes of the transsulfuration and assimilatory pathways, respectively, from the hemoflagellate protozoan parasite Trypanosoma rangeli. RESULTS: Our results show that T. rangeli CβS (TrCβS), similar to its homologs in T. cruzi, contains the catalytic domain essential for enzymatic activity. Unlike the enzymes in bacteria, plants, and other parasites, T. rangeli CS lacks two of the four lysine residues (Lys26 and Lys184) required for activity. Enzymatic studies using T. rangeli extracts confirmed the absence of CS activity but confirmed the expression of an active CβS. Moreover, CβS biochemical assays revealed that the T. rangeli CβS enzyme also has serine sulfhydrylase activity. CONCLUSION: These findings demonstrate that the RTS pathway is active in T. rangeli, suggesting that this may be the only pathway for cysteine biosynthesis in this parasite. In this sense, the RTS pathway appears to have an important functional role during the insect stage of the life cycle of this protozoan parasite.Cysteine biosynthesisCystathionine β-synthaseCysteine synthaseT. rangeliThiol metabolismAntioxidant defenceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9479/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_069.pdf2014_069.pdfapplication/pdf2576997https://www.arca.fiocruz.br/bitstream/icict/9479/2/2014_069.pdf077200f211bbadff1c1319e785d3bed7MD52TEXT2014_069.pdf.txt2014_069.pdf.txtExtracted texttext/plain46363https://www.arca.fiocruz.br/bitstream/icict/9479/3/2014_069.pdf.txt46b3545e7eaa62578e13fb21bc85d20fMD53icict/94792019-06-19 10:07:36.603oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
title Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
spellingShingle Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
Romero, Ibeth
Cysteine biosynthesis
Cystathionine β-synthase
Cysteine synthase
T. rangeli
Thiol metabolism
Antioxidant defence
title_short Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
title_full Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
title_fullStr Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
title_full_unstemmed Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
title_sort Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli
author Romero, Ibeth
author_facet Romero, Ibeth
Téllez, Jair
Yamanaka, Lais Eiko
Steindel, Mario
Romanha, Alvaro José
Grisard, Edmundo Carlos
author_role author
author2 Téllez, Jair
Yamanaka, Lais Eiko
Steindel, Mario
Romanha, Alvaro José
Grisard, Edmundo Carlos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Romero, Ibeth
Téllez, Jair
Yamanaka, Lais Eiko
Steindel, Mario
Romanha, Alvaro José
Grisard, Edmundo Carlos
dc.subject.en.pt_BR.fl_str_mv Cysteine biosynthesis
Cystathionine β-synthase
Cysteine synthase
T. rangeli
Thiol metabolism
Antioxidant defence
topic Cysteine biosynthesis
Cystathionine β-synthase
Cysteine synthase
T. rangeli
Thiol metabolism
Antioxidant defence
description Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia e de Bioinformática. Florianópolis, SC, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-02-10T17:26:26Z
dc.date.available.fl_str_mv 2015-02-10T17:26:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROMERO, Ibeth et al. Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli. Parasites & Vectors, v. 7, n. 1, p. 197, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9479
dc.identifier.issn.none.fl_str_mv 1756-3305
identifier_str_mv ROMERO, Ibeth et al. Transsulfuration is an active pathway for cysteine biosynthesis in Trypanosoma rangeli. Parasites & Vectors, v. 7, n. 1, p. 197, 2014.
1756-3305
url https://www.arca.fiocruz.br/handle/icict/9479
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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