Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Kalb, Ligia Cristina
Data de Publicação: 2016
Outros Autores: Frederico, Yohana Camila Antunes, Boehm, Corrdula, Moreira, Claudia Maria do Nascimento, Soares, Maurilio José, Field, Mark C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20421
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil.
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spelling Kalb, Ligia CristinaFrederico, Yohana Camila AntunesBoehm, CorrdulaMoreira, Claudia Maria do NascimentoSoares, Maurilio JoséField, Mark C.2017-07-27T12:05:39Z2017-07-27T12:05:39Z2016KALB, Ligia Cristina et al. Conservation and divergence within the clathrin interactome of Trypanosoma cruzi. Scientific Reports v. 6, n. 31212, p. 1-12. 9 Aug. 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/2042110.1038/srep31212engNature Publishing GroupTrypanosoma cruziEndocytosisClathrinAdaptor Protein Complex 2EndocitosisClatrinaComplejo 2 de Proteína AdaptadoraTrypanosoma cruziEndocitoseClatrinaComplexo 2 de Proteínas AdaptadorasConservation and divergence within the Clathrin interactome of Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil.School of Life Sciences. University of Dundee. Dundee, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil / School of Life Sciences. University of Dundee. Dundee, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil.School of Life Sciences. University of Dundee. Dundee, UK.Trypanosomatids are parasitic protozoa with a significant burden on human health. African and American trypanosomes are causative agents of Nagana and Chagas disease respectively, and speciated about 300 million years ago. These parasites have highly distinct life cycles, pathologies, transmission strategies and surface proteomes, being dominated by the variant surface glycoprotein (African) or mucins (American) respectively. In African trypanosomes clathrin-mediated trafficking is responsible for endocytosis and post-Golgi transport, with several mechanistic aspects distinct from higher organisms. Using clathrin light chain (TcCLC) and EpsinR (TcEpsinR) as affinity handles, we identified candidate clathrin-associated proteins (CAPs) in Trypanosoma cruzi; the cohort includes orthologs of many proteins known to mediate vesicle trafficking, but significantly not the AP-2 adaptor complex. Several trypanosome-specific proteins common with African trypanosomes, were also identified. Fluorescence microscopy revealed localisations for TcEpsinR, TcCLC and TcCHC at the posterior region of trypomastigote cells, coincident with the flagellar pocket and Golgi apparatus. These data provide the first systematic analysis of clathrin-mediated trafficking in T. cruzi, allowing comparison between protein cohorts and other trypanosomes and also suggest that clathrin trafficking in at least some life stages of T. cruzi may be AP-2-independent.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/20421/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALCONSERVATION AND DIVERGENCE WITHIN THE CLATHRIN INTERACTOME OF TRYPANOSOMA CRUZI.pdfCONSERVATION AND DIVERGENCE WITHIN THE CLATHRIN INTERACTOME OF TRYPANOSOMA CRUZI.pdfapplication/pdf1054994https://www.arca.fiocruz.br/bitstream/icict/20421/2/CONSERVATION%20AND%20DIVERGENCE%20WITHIN%20THE%20CLATHRIN%20INTERACTOME%20OF%20TRYPANOSOMA%20CRUZI.pdfe4f8555fff924dce0e05ab2d463c6373MD52TEXTCONSERVATION AND DIVERGENCE WITHIN THE 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dc.title.pt_BR.fl_str_mv Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
title Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
spellingShingle Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
Kalb, Ligia Cristina
Trypanosoma cruzi
Endocytosis
Clathrin
Adaptor Protein Complex 2
Endocitosis
Clatrina
Complejo 2 de Proteína Adaptadora
Trypanosoma cruzi
Endocitose
Clatrina
Complexo 2 de Proteínas Adaptadoras
title_short Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
title_full Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
title_fullStr Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
title_full_unstemmed Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
title_sort Conservation and divergence within the Clathrin interactome of Trypanosoma cruzi
author Kalb, Ligia Cristina
author_facet Kalb, Ligia Cristina
Frederico, Yohana Camila Antunes
Boehm, Corrdula
Moreira, Claudia Maria do Nascimento
Soares, Maurilio José
Field, Mark C.
author_role author
author2 Frederico, Yohana Camila Antunes
Boehm, Corrdula
Moreira, Claudia Maria do Nascimento
Soares, Maurilio José
Field, Mark C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Kalb, Ligia Cristina
Frederico, Yohana Camila Antunes
Boehm, Corrdula
Moreira, Claudia Maria do Nascimento
Soares, Maurilio José
Field, Mark C.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
Endocytosis
Clathrin
Adaptor Protein Complex 2
Endocitosis
Clatrina
Complejo 2 de Proteína Adaptadora
Trypanosoma cruzi
Endocitose
Clatrina
Complexo 2 de Proteínas Adaptadoras
dc.subject.en.pt_BR.fl_str_mv Endocytosis
Clathrin
Adaptor Protein Complex 2
dc.subject.es.pt_BR.fl_str_mv Endocitosis
Clatrina
Complejo 2 de Proteína Adaptadora
dc.subject.decs.pt_BR.fl_str_mv Trypanosoma cruzi
Endocitose
Clatrina
Complexo 2 de Proteínas Adaptadoras
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular, Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanosomas, Curitiba, PR, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-07-27T12:05:39Z
dc.date.available.fl_str_mv 2017-07-27T12:05:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KALB, Ligia Cristina et al. Conservation and divergence within the clathrin interactome of Trypanosoma cruzi. Scientific Reports v. 6, n. 31212, p. 1-12. 9 Aug. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20421
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.pt_BR.fl_str_mv 10.1038/srep31212
identifier_str_mv KALB, Ligia Cristina et al. Conservation and divergence within the clathrin interactome of Trypanosoma cruzi. Scientific Reports v. 6, n. 31212, p. 1-12. 9 Aug. 2016.
2045-2322
10.1038/srep31212
url https://www.arca.fiocruz.br/handle/icict/20421
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/20421/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/20421/2/CONSERVATION%20AND%20DIVERGENCE%20WITHIN%20THE%20CLATHRIN%20INTERACTOME%20OF%20TRYPANOSOMA%20CRUZI.pdf
https://www.arca.fiocruz.br/bitstream/icict/20421/3/CONSERVATION%20AND%20DIVERGENCE%20WITHIN%20THE%20CLATHRIN%20INTERACTOME%20OF%20TRYPANOSOMA%20CRUZI.pdf.txt
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