The calmodulin intergenic spacer as molecular target for characterization of Leishmania species

Detalhes bibliográficos
Autor(a) principal: Miranda, Aracelis
Data de Publicação: 2014
Outros Autores: Samudio, Franklin, Saldaña, Azael, Castillo, Juan, Brandão, Adeilton, Calzada, Jose E
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10861
Resumo: Instituto Conmemorativo Gorgas de Estudios de Salud. Ciudad de Panamá, Panamá
id CRUZ_17fc1d3aa8ece49a70ea30b0b9d178df
oai_identifier_str oai:www.arca.fiocruz.br:icict/10861
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Miranda, AracelisSamudio, FranklinSaldaña, AzaelCastillo, JuanBrandão, AdeiltonCalzada, Jose E2015-06-17T12:05:19Z2015-06-17T12:05:19Z2014MIRANDA, Aracelis et al. The calmodulin intergenic spacer as molecular target for characterization of Leishmania species. Parasites & Vectors, v.7:35, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/1086110.1186/1756-3305-7-35engBioMed CentralThe calmodulin intergenic spacer as molecular target for characterization of Leishmania speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Conmemorativo Gorgas de Estudios de Salud. Ciudad de Panamá, PanamáInstituto Conmemorativo Gorgas de Estudios de Salud. Ciudad de Panamá, Panamá / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Instituto Conmemorativo Gorgas de Estudios de Salud. Ciudad de Panamá, PanamáInstituto Conmemorativo Gorgas de Estudios de Salud. Ciudad de Panamá, Panamá.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Instituto Conmemorativo Gorgas de Estudios de Salud. Ciudad de Panamá, PanamáBackground: Human leishmaniasis is a neglected disease caused by parasites of the genus Leishmania. Clinical aspects of this disease can vary significantly, reflecting the wide range of parasites in the genus Leishmania. Knowing accurately the Leishmania species infecting humans is important for clinical case management and evaluation of epidemiological risk. Calmodulin is an essential gene in trypanosomatids that modulates the calcium metabolism in various cellular activities. Despite its strong conservation in trypanosomatids, it has been recently observed that its untranslated regions (UTR) diverge among species. Methods: In this study we analyzed the sequences and the absolute dinucleotide frequency of the intergenic spacer of the calmodulin gene (containing both, 3′ and 5′UTR) in nine reference Leishmania species and ten clinical isolates obtained from patients with cutaneous leishmaniasis. Results: We show that the short calmodulin intergenic spacers exhibit features that make them interesting for applications in molecular characterization and phylogenetic studies of Leishmania. Dendrograms based on sequence alignments and on the dinucleotide frequency indicate that this particular region of calmodulin gene might be useful for species typing between the Leishmania and Viannia subgenera. Conclusions: Mutations and composition of the calmodulin intergenic spacer from Leishmania species might have taxonomic value as parameters to define if an isolate is identical to a certain species or belongs to one of the two current subgenera.LeishmaniaCalmodulinIntergenic spacer3`UTRPhylogenetic analysisPanamaLeishmaniaCalmodulinainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10861/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALadeilton_brandãoetal_IOC_2014.pdfapplication/pdf1322090https://www.arca.fiocruz.br/bitstream/icict/10861/2/adeilton_brand%c3%a3oetal_IOC_2014.pdfcb40a22cda00df4c32983c4024cc9698MD52TEXTadeilton_brandãoetal_IOC_2014.pdf.txtadeilton_brandãoetal_IOC_2014.pdf.txtExtracted texttext/plain28234https://www.arca.fiocruz.br/bitstream/icict/10861/3/adeilton_brand%c3%a3oetal_IOC_2014.pdf.txtbbe28c7689900f297942bf52d7fced68MD53icict/108612022-06-24 13:08:46.527oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:46Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
title The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
spellingShingle The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
Miranda, Aracelis
Leishmania
Calmodulin
Intergenic spacer
3`UTR
Phylogenetic analysis
Panama
Leishmania
Calmodulina
title_short The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
title_full The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
title_fullStr The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
title_full_unstemmed The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
title_sort The calmodulin intergenic spacer as molecular target for characterization of Leishmania species
author Miranda, Aracelis
author_facet Miranda, Aracelis
Samudio, Franklin
Saldaña, Azael
Castillo, Juan
Brandão, Adeilton
Calzada, Jose E
author_role author
author2 Samudio, Franklin
Saldaña, Azael
Castillo, Juan
Brandão, Adeilton
Calzada, Jose E
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Miranda, Aracelis
Samudio, Franklin
Saldaña, Azael
Castillo, Juan
Brandão, Adeilton
Calzada, Jose E
dc.subject.en.pt_BR.fl_str_mv Leishmania
Calmodulin
Intergenic spacer
3`UTR
Phylogenetic analysis
Panama
topic Leishmania
Calmodulin
Intergenic spacer
3`UTR
Phylogenetic analysis
Panama
Leishmania
Calmodulina
dc.subject.decs.pt_BR.fl_str_mv Leishmania
Calmodulina
description Instituto Conmemorativo Gorgas de Estudios de Salud. Ciudad de Panamá, Panamá
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-17T12:05:19Z
dc.date.available.fl_str_mv 2015-06-17T12:05:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MIRANDA, Aracelis et al. The calmodulin intergenic spacer as molecular target for characterization of Leishmania species. Parasites & Vectors, v.7:35, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10861
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/1756-3305-7-35
identifier_str_mv MIRANDA, Aracelis et al. The calmodulin intergenic spacer as molecular target for characterization of Leishmania species. Parasites & Vectors, v.7:35, 2014.
1756-3305
10.1186/1756-3305-7-35
url https://www.arca.fiocruz.br/handle/icict/10861
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10861/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/10861/2/adeilton_brand%c3%a3oetal_IOC_2014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10861/3/adeilton_brand%c3%a3oetal_IOC_2014.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
cb40a22cda00df4c32983c4024cc9698
bbe28c7689900f297942bf52d7fced68
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324872647016448

Registros relacionados