Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13118 http://dx.doi.org/10.3389/fmicb.2015.00860 |
Resumo: | Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. |
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Pinheiro, Guilherme L.Correa, Raquel F.Cunha, Raquel S.Cardoso, Alexander MachadoChaia, CatiaClementino, Maysa Beatriz MandettaGarcia, Eloi de SouzaSouza, Wanderley deFrasés, Susana2016-03-10T17:45:42Z2016-03-10T17:45:42Z2015PINHEIRO, G. L. et al. Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica. Front. Microbiol., v. 6, n. 860, 2015.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/13118http://dx.doi.org/10.3389/fmicb.2015.00860engFrontiers MediaAchatina fulicaBactérias CelulolíticasCarboximetilceluloseDiversidade MicrobianaAchatina fulicaCellulolytic BacteriaCarboxymethycelluloseMicrobial DiversityCaramujosClostridium cellulolyticumCarboximetilcelulose SódicaDiversidade MicrobianaIsolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Centro Universitário Estadual da Zona Oeste. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.The enzymatic hydrolysis of cellulose by cellulases is one of the major limiting steps in the conversion of lignocellulosic biomass to yield bioethanol. To overcome this hindrance, significant efforts are underway to identify novel cellulases. The snail Achatina fulica is a gastropod with high cellulolytic activity, mainly due to the abundance of glycoside hydrolases produced by both the animal and its resident microbiota. In this study, we partially assessed the cellulolytic aerobic bacterial diversity inside the gastrointestinal tract of A. fulica by culture-dependent methods and evaluated the hydrolytic repertoire of the isolates. Forty bacterial isolates were recovered from distinct segments of the snail gut and identified to the genus level by 16S rRNA gene sequence analysis. Additional phenotypic characterization was performed using biochemical tests provided by the Vitek2 identification system. The overall enzymatic repertoire of the isolated strains was investigated by enzymatic plate assays, containing the following substrates: powdered sugarcane bagasse, carboxymethylcellulose (CMC), p-nitrophenyl-beta-D-glucopyranoside (pNPG), p-nitrophenyl-beta-D-cellobioside (pNPC), 4-methylumbelliferyl-beta-D-glucopyranoside (MUG), 4-methylumbelliferyl-beta-D-cellobioside (MUC), and 4-methylumbelliferyl-beta-D-xylopyranoside (MUX). Our results indicate that the snail A. fulica is an attractive source of cultivable bacteria that showed to be valuable resources for the production of different types of biomass-degrading enzymes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13118/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfmicb-06-00860.pdffmicb-06-00860.pdfapplication/pdf1456467https://www.arca.fiocruz.br/bitstream/icict/13118/2/fmicb-06-00860.pdf8046b0dd63675ecfbdcf95d9a719403aMD52TEXTfmicb-06-00860.pdf.txtfmicb-06-00860.pdf.txtExtracted texttext/plain74067https://www.arca.fiocruz.br/bitstream/icict/13118/3/fmicb-06-00860.pdf.txt2765e08af08f92368c3826f50501a174MD53icict/131182018-04-02 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| dc.title.en.fl_str_mv |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica |
| title |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica |
| spellingShingle |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica Pinheiro, Guilherme L. Achatina fulica Bactérias Celulolíticas Carboximetilcelulose Diversidade Microbiana Achatina fulica Cellulolytic Bacteria Carboxymethycellulose Microbial Diversity Caramujos Clostridium cellulolyticum Carboximetilcelulose Sódica Diversidade Microbiana |
| title_short |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica |
| title_full |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica |
| title_fullStr |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica |
| title_full_unstemmed |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica |
| title_sort |
Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica |
| author |
Pinheiro, Guilherme L. |
| author_facet |
Pinheiro, Guilherme L. Correa, Raquel F. Cunha, Raquel S. Cardoso, Alexander Machado Chaia, Catia Clementino, Maysa Beatriz Mandetta Garcia, Eloi de Souza Souza, Wanderley de Frasés, Susana |
| author_role |
author |
| author2 |
Correa, Raquel F. Cunha, Raquel S. Cardoso, Alexander Machado Chaia, Catia Clementino, Maysa Beatriz Mandetta Garcia, Eloi de Souza Souza, Wanderley de Frasés, Susana |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pinheiro, Guilherme L. Correa, Raquel F. Cunha, Raquel S. Cardoso, Alexander Machado Chaia, Catia Clementino, Maysa Beatriz Mandetta Garcia, Eloi de Souza Souza, Wanderley de Frasés, Susana |
| dc.subject.other.pt_BR.fl_str_mv |
Achatina fulica Bactérias Celulolíticas Carboximetilcelulose Diversidade Microbiana |
| topic |
Achatina fulica Bactérias Celulolíticas Carboximetilcelulose Diversidade Microbiana Achatina fulica Cellulolytic Bacteria Carboxymethycellulose Microbial Diversity Caramujos Clostridium cellulolyticum Carboximetilcelulose Sódica Diversidade Microbiana |
| dc.subject.en.en.fl_str_mv |
Achatina fulica Cellulolytic Bacteria Carboxymethycellulose Microbial Diversity |
| dc.subject.decs.pt_BR.fl_str_mv |
Caramujos Clostridium cellulolyticum Carboximetilcelulose Sódica Diversidade Microbiana |
| description |
Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. |
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2015 |
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2015 |
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2016-03-10T17:45:42Z |
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2016-03-10T17:45:42Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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PINHEIRO, G. L. et al. Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica. Front. Microbiol., v. 6, n. 860, 2015. |
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https://www.arca.fiocruz.br/handle/icict/13118 |
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1664-302X |
| dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2015.00860 |
| identifier_str_mv |
PINHEIRO, G. L. et al. Isolation of aerobic cultivable cellulolytic bacteria from different regions of the gastrointestinal tract of giant land snail Achatina fulica. Front. Microbiol., v. 6, n. 860, 2015. 1664-302X |
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Frontiers Media |
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Frontiers Media |
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