Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route

Oliveira, Edson R. A.; Gonçalves, Antônio J. S.; Costa, Simone M.; Azevedo, Adriana S.; Mantuano-Barradas, Marcio; Nogueira, Ana Cristina M. A.; Alves, Ada M. B.

Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route

Detalhes bibliográficos
Autor(a) principal:	Oliveira, Edson R. A.
Data de Publicação:	2016
Outros Autores:	Gonçalves, Antônio J. S., Costa, Simone M., Azevedo, Adriana S., Mantuano-Barradas, Marcio, Nogueira, Ana Cristina M. A., Alves, Ada M. B.
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/17832
Resumo:	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.

Metadados do item

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spelling	Oliveira, Edson R. A.Gonçalves, Antônio J. S.Costa, Simone M.Azevedo, Adriana S.Mantuano-Barradas, MarcioNogueira, Ana Cristina M. A.Alves, Ada M. B.2017-02-16T11:51:04Z2017-02-16T11:51:04Z2016OLIVEIRA, Edson R. A. et al. Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route. . PLoS ONE, v.11, n.9, e0163240, 19p, Sept. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1783210.1371/journal.pone.0163240engPublic Library of ScienceDengueSaúde PúblicaVacina de DNAImunidade mediada por células TDenguePublic healthDNA vaccineT Cell-Mediated ImmunityAspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Routeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clìnica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.Dengue disease has emerged as a major public health issue across tropical and subtropical countries. Infections caused by dengue virus (DENV) can evolve to life-threatening forms, resulting in about 20,000 deaths every year worldwide. Several animal models have been described concerning pre-clinical stages in vaccine development against dengue, each of them presenting limitations and advantages. Among these models, a traditional approach is the inoculation of a mouse-brain adapted DENV variant in immunocompetent animals by the intracerebral (i.c.) route. Despite the historical usage and relevance of this model for vaccine testing, little is known about the mechanisms by which the protection is developed upon vaccination. To cover this topic, a DNA vaccine based on the DENV non-structural protein 1 (pcTPANS1) was considered and investigations were focused on the induced T cell-mediated immunity against i.c.-DENV infection. Immunophenotyping assays by flow cytometry revealed that immunization with pcTPANS1 promotes a sustained T cell activation in spleen of i.c.-infected mice. Moreover, we found that the downregulation of CD45RB on T cells, as an indicator of cell activation, correlated with absence of morbidity upon virus challenge. Adoptive transfer procedures supported by CFSE-labeled cell tracking showed that NS1-specific T cells induced by vaccination, proliferate and migrate to peripheral organs of infected mice, such as the liver. Additionally, in late stages of infection (from the 7th day onwards), vaccinated mice also presented reduced levels of circulating IFN-γ and IL-12p70 in comparison to non-vaccinated animals. In conclusion, this work presented new aspects about the T cell-mediated immunity concerning DNA vaccination with pcTPANS1 and the i.c. infection model. These insights can be explored in further studies of anti-dengue vaccine efficacy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17832/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALedson_oliveira_etal_IOC_2016.pdfedson_oliveira_etal_IOC_2016.pdfapplication/pdf2481802https://www.arca.fiocruz.br/bitstream/icict/17832/2/edson_oliveira_etal_IOC_2016.pdf94775663ee3222818c039d9e1a115b52MD52TEXTedson_oliveira_etal_IOC_2016.pdf.txtedson_oliveira_etal_IOC_2016.pdf.txtExtracted texttext/plain62282https://www.arca.fiocruz.br/bitstream/icict/17832/3/edson_oliveira_etal_IOC_2016.pdf.txt252ad01e08920cb96d0e865249431a89MD53icict/178322018-08-15 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dc.title.pt_BR.fl_str_mv	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route
title	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route
spellingShingle	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route Oliveira, Edson R. A. Dengue Saúde Pública Vacina de DNA Imunidade mediada por células T Dengue Public health DNA vaccine T Cell-Mediated Immunity
title_short	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route
title_full	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route
title_fullStr	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route
title_full_unstemmed	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route
title_sort	Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route
author	Oliveira, Edson R. A.
author_facet	Oliveira, Edson R. A. Gonçalves, Antônio J. S. Costa, Simone M. Azevedo, Adriana S. Mantuano-Barradas, Marcio Nogueira, Ana Cristina M. A. Alves, Ada M. B.
author_role	author
author2	Gonçalves, Antônio J. S. Costa, Simone M. Azevedo, Adriana S. Mantuano-Barradas, Marcio Nogueira, Ana Cristina M. A. Alves, Ada M. B.
author2_role	author author author author author author
dc.contributor.author.fl_str_mv	Oliveira, Edson R. A. Gonçalves, Antônio J. S. Costa, Simone M. Azevedo, Adriana S. Mantuano-Barradas, Marcio Nogueira, Ana Cristina M. A. Alves, Ada M. B.
dc.subject.other.pt_BR.fl_str_mv	Dengue Saúde Pública Vacina de DNA Imunidade mediada por células T
topic	Dengue Saúde Pública Vacina de DNA Imunidade mediada por células T Dengue Public health DNA vaccine T Cell-Mediated Immunity
dc.subject.en.pt_BR.fl_str_mv	Dengue Public health DNA vaccine T Cell-Mediated Immunity
description	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.
publishDate	2016
dc.date.issued.fl_str_mv	2016
dc.date.accessioned.fl_str_mv	2017-02-16T11:51:04Z
dc.date.available.fl_str_mv	2017-02-16T11:51:04Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	OLIVEIRA, Edson R. A. et al. Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route. . PLoS ONE, v.11, n.9, e0163240, 19p, Sept. 2016.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/17832
dc.identifier.issn.pt_BR.fl_str_mv	1932-6203
dc.identifier.doi.none.fl_str_mv	10.1371/journal.pone.0163240
identifier_str_mv	OLIVEIRA, Edson R. A. et al. Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route. . PLoS ONE, v.11, n.9, e0163240, 19p, Sept. 2016. 1932-6203 10.1371/journal.pone.0163240
url	https://www.arca.fiocruz.br/handle/icict/17832
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/17832/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/17832/2/edson_oliveira_etal_IOC_2016.pdf https://www.arca.fiocruz.br/bitstream/icict/17832/3/edson_oliveira_etal_IOC_2016.pdf.txt
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Aspects of T Cell-Mediated Immunity Induced in Mice by a DNA Vaccine Based on the Dengue-NS1 Antigen after Challenge by the Intracerebral Route

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