BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/55793 https://doi.org/10.1016/j.envpol.2022.120140 |
Resumo: | Universidade Federal do Paraná. Departamento de Biologia Celular. Laboratório de Toxicologia Celular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. |
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Silva Filho, Benisio FerreiraFilipak Neto, FranciscoMarchi, Micheli deMoggio, Erick LaurentRossi, Izadora VolpatoSabatke, BrunaRamírez, Marcel IvanLucena, Miguel Clodomiro dos SantosTodeschini, Adriane ReginaRibeiro, Ciro Alberto de Oliveira2022-11-25T15:19:42Z2022-11-25T15:19:42Z2022SILVA FILHO, Benisio Ferreira et al. BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure. Environmental Pollution, v. 313, n. 120140, p. 1–10 2022.1873-6424https://www.arca.fiocruz.br/handle/icict/55793https://doi.org/10.1016/j.envpol.2022.120140porElsevierTCDDBDE-219MetalloproteasesEpithelial-Mesenchymal TransitionExtracellular Vesicles2,3,7,8-Tetraclorodibenzo-p-dioxinaHydrocarbons, BrominatedDioxinsCarcinogenesisMetaloproteasasTransición Epitelial-MesenquimalVesículas ExtracelularesDibenzodioxinas PolicloradasHidrocarburos BromadosCarcinogénesisMetaloproteasesTransição Epitelial-MesenquimalVesículas ExtracelularesDibenzodioxinas PolicloradasHidrocarbonetos BromadosDioxinasCarcinogêneseBDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Biologia Celular. Laboratório de Toxicologia Celular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Laboratório de Toxicologia Celular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Laboratório de Toxicologia Celular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Laboratório de Toxicologia Celular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Microbiologia, Parasitologia e Patologia. Curitiba, PR, Brasil.Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Microbiologia, Parasitologia e Patologia. Curitiba, PR, Brasil.Universidade Federal do Rio de Janeiro. Instituto Carlos Chagas Filho. Laboratório de Glicobiologia Estrutural e Funcional. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto Carlos Chagas Filho. Laboratório de Glicobiologia Estrutural e Funcional. Rio de Janeiro, RJ, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Laboratório de Toxicologia Celular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil.TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and BDE-209 (decabromodiphenyl ether) are persistent organic pollutants (POPs) produced by industrial activities and associated with several diseases. TCDD is a known human carcinogen, but few studies investigated about the effects of exposure to both compounds, i.e., whether BDE-209 and TCDD can render tumor cells more aggressive and metastatic. In the current study we investigated if the exposure of B16–F1 and B16–F10 melanoma murine cells to environmental relevant concentrations of TCDD and BDE-209 at 24 h and 15-day exposure modulates the expression of genes related to metastasis, making the cells more aggressive. Both pollutants did not affect cell viability but lead to increase of cell proliferation, including the upregulation of vimentin, MMP2, MMP9, MMP14 and PGK1 gene expression and downregulation of Ecadherin, TIMP2, TIMP3 and RECK, strongly suggesting changes in cell phenotypes defined as epithelial to mesenchymal transition (EMT) in BDE-209 and TCDD-exposed cells. Foremost, increased expression of metalloproteinases and decreased expression of their inhibitors made B16–F1 cells similar the more aggressive B16–F10 cell line. Also, the higher secretion of extracellular vesicles by cells after acute exposure to BDE-209 could be related with the phenotype changes. These results are a strong indication of the potential of BDE209 and TCDD to modulate cell phenotype, leading to a more aggressive profile.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55793/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL1-s2.0-S0269749122013549-mainok.pdf1-s2.0-S0269749122013549-mainok.pdfapplication/pdf6084855https://www.arca.fiocruz.br/bitstream/icict/55793/2/1-s2.0-S0269749122013549-mainok.pdf5412a7ad4eee3eca45fd67193ad1a0d5MD52icict/557932022-12-16 15:30:58.305oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-12-16T18:30:58Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure |
| title |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure |
| spellingShingle |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure Silva Filho, Benisio Ferreira TCDD BDE-219 Metalloproteases Epithelial-Mesenchymal Transition Extracellular Vesicles 2,3,7,8-Tetraclorodibenzo-p-dioxina Hydrocarbons, Brominated Dioxins Carcinogenesis Metaloproteasas Transición Epitelial-Mesenquimal Vesículas Extracelulares Dibenzodioxinas Policloradas Hidrocarburos Bromados Carcinogénesis Metaloproteases Transição Epitelial-Mesenquimal Vesículas Extracelulares Dibenzodioxinas Policloradas Hidrocarbonetos Bromados Dioxinas Carcinogênese |
| title_short |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure |
| title_full |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure |
| title_fullStr |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure |
| title_full_unstemmed |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure |
| title_sort |
BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure |
| author |
Silva Filho, Benisio Ferreira |
| author_facet |
Silva Filho, Benisio Ferreira Filipak Neto, Francisco Marchi, Micheli de Moggio, Erick Laurent Rossi, Izadora Volpato Sabatke, Bruna Ramírez, Marcel Ivan Lucena, Miguel Clodomiro dos Santos Todeschini, Adriane Regina Ribeiro, Ciro Alberto de Oliveira |
| author_role |
author |
| author2 |
Filipak Neto, Francisco Marchi, Micheli de Moggio, Erick Laurent Rossi, Izadora Volpato Sabatke, Bruna Ramírez, Marcel Ivan Lucena, Miguel Clodomiro dos Santos Todeschini, Adriane Regina Ribeiro, Ciro Alberto de Oliveira |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva Filho, Benisio Ferreira Filipak Neto, Francisco Marchi, Micheli de Moggio, Erick Laurent Rossi, Izadora Volpato Sabatke, Bruna Ramírez, Marcel Ivan Lucena, Miguel Clodomiro dos Santos Todeschini, Adriane Regina Ribeiro, Ciro Alberto de Oliveira |
| dc.subject.other.en_US.fl_str_mv |
TCDD BDE-219 |
| topic |
TCDD BDE-219 Metalloproteases Epithelial-Mesenchymal Transition Extracellular Vesicles 2,3,7,8-Tetraclorodibenzo-p-dioxina Hydrocarbons, Brominated Dioxins Carcinogenesis Metaloproteasas Transición Epitelial-Mesenquimal Vesículas Extracelulares Dibenzodioxinas Policloradas Hidrocarburos Bromados Carcinogénesis Metaloproteases Transição Epitelial-Mesenquimal Vesículas Extracelulares Dibenzodioxinas Policloradas Hidrocarbonetos Bromados Dioxinas Carcinogênese |
| dc.subject.en.en_US.fl_str_mv |
Metalloproteases Epithelial-Mesenchymal Transition Extracellular Vesicles 2,3,7,8-Tetraclorodibenzo-p-dioxina Hydrocarbons, Brominated Dioxins Carcinogenesis |
| dc.subject.es.en_US.fl_str_mv |
Metaloproteasas Transición Epitelial-Mesenquimal Vesículas Extracelulares Dibenzodioxinas Policloradas Hidrocarburos Bromados Carcinogénesis |
| dc.subject.decs.en_US.fl_str_mv |
Metaloproteases Transição Epitelial-Mesenquimal Vesículas Extracelulares Dibenzodioxinas Policloradas Hidrocarbonetos Bromados Dioxinas Carcinogênese |
| description |
Universidade Federal do Paraná. Departamento de Biologia Celular. Laboratório de Toxicologia Celular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. |
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2022 |
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2022-11-25T15:19:42Z |
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2022-11-25T15:19:42Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA FILHO, Benisio Ferreira et al. BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure. Environmental Pollution, v. 313, n. 120140, p. 1–10 2022. |
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https://www.arca.fiocruz.br/handle/icict/55793 |
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1873-6424 |
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https://doi.org/10.1016/j.envpol.2022.120140 |
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SILVA FILHO, Benisio Ferreira et al. BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure. Environmental Pollution, v. 313, n. 120140, p. 1–10 2022. 1873-6424 |
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Elsevier |
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