A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases

Detalhes bibliográficos
Autor(a) principal: Alves, Carlos Roberto
Data de Publicação: 2001
Outros Autores: Pontes-de-Carvalho, Lain Carlos, Souza, André Luis Almeida, De Simone, Salvatore Giovanni
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20388
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do estado do Rio de Janeiro (FAPERJ) and FIOCRUZ.
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spelling Alves, Carlos RobertoPontes-de-Carvalho, Lain CarlosSouza, André Luis AlmeidaDe Simone, Salvatore Giovanni2017-07-25T13:16:34Z2017-07-25T13:16:34Z2001ALVES, Carlos Roberto et al. A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases. Cytobios, v. 104, p. 33-41, 2001.0011-4529https://www.arca.fiocruz.br/handle/icict/20388Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do estado do Rio de Janeiro (FAPERJ) and FIOCRUZ.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteinases e Peptídeos. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteinases e Peptídeos. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteinases e Peptídeos. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.In this study computational analysis was used to compile sequence alignments, constructo a dendrogram and calculate physical data in order to predict potential T-cell epitopes of the Leishmania cysteine proteinase. Using multiple alignment of human and Leishmania proteinase sequences deposited on data bank sequences, it was possible to predict that the extreme C-terminus of cysteine proteinase (Cyspep, 355-444) contained three peptides (pl 361-370, pll 415-422 and plll 431-444) with charge score, hydrophobicity and isoelectric points compatible for human leucocyte-associated antigen (HLA) class II binding. The prediction was confirmed in vitro through the ability of synthetic peptides corresponding to the predicted regions to stimulate peripheral blood mononuclear cells of patients with leishmaniasis.engLeishmaniaCisteina proteinasesEpitoposLeishmania braziliensisHumanosLeishmaniaCysteine proteinasesEpitopesLeishmania braziliensisHumansA strategy for the identification of T-cell epitopes on Leishmania cysteine proteinasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20388/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlves C A strategy for the ....pdfAlves C A strategy for the 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dc.title.pt_BR.fl_str_mv A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
title A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
spellingShingle A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
Alves, Carlos Roberto
Leishmania
Cisteina proteinases
Epitopos
Leishmania braziliensis
Humanos
Leishmania
Cysteine proteinases
Epitopes
Leishmania braziliensis
Humans
title_short A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
title_full A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
title_fullStr A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
title_full_unstemmed A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
title_sort A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases
author Alves, Carlos Roberto
author_facet Alves, Carlos Roberto
Pontes-de-Carvalho, Lain Carlos
Souza, André Luis Almeida
De Simone, Salvatore Giovanni
author_role author
author2 Pontes-de-Carvalho, Lain Carlos
Souza, André Luis Almeida
De Simone, Salvatore Giovanni
author2_role author
author
author
dc.contributor.author.fl_str_mv Alves, Carlos Roberto
Pontes-de-Carvalho, Lain Carlos
Souza, André Luis Almeida
De Simone, Salvatore Giovanni
dc.subject.other.pt_BR.fl_str_mv Leishmania
Cisteina proteinases
Epitopos
Leishmania braziliensis
Humanos
topic Leishmania
Cisteina proteinases
Epitopos
Leishmania braziliensis
Humanos
Leishmania
Cysteine proteinases
Epitopes
Leishmania braziliensis
Humans
dc.subject.en.pt_BR.fl_str_mv Leishmania
Cysteine proteinases
Epitopes
Leishmania braziliensis
Humans
description Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do estado do Rio de Janeiro (FAPERJ) and FIOCRUZ.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2017-07-25T13:16:34Z
dc.date.available.fl_str_mv 2017-07-25T13:16:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Carlos Roberto et al. A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases. Cytobios, v. 104, p. 33-41, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20388
dc.identifier.issn.pt_BR.fl_str_mv 0011-4529
identifier_str_mv ALVES, Carlos Roberto et al. A strategy for the identification of T-cell epitopes on Leishmania cysteine proteinases. Cytobios, v. 104, p. 33-41, 2001.
0011-4529
url https://www.arca.fiocruz.br/handle/icict/20388
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
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