Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/52085 |
Resumo: | Universidade Regional do Cariri. Laboratório de Bioprospecção do Semiárido. Crato, CE, Brasil. |
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Lopes, Thais PereiraBarbosa, Cristina Rodrigues dos SantosMacêdo, Nair SilvaScherf, Jackelyne RobertaSantos, Maria Apoliana Costa dosFreitas, Thiago Sampaio deRibeiro Filho, JaimeCoutinho, Henrique Douglas MeloSiyadatpanah, AbolghasemWilairatana, PolratCunha, Francisco Assis Bezerra daPinheiro, Alysson Pontes2022-04-07T14:26:25Z2022-04-07T14:26:25Z2022LOPES, Thais Pereira et al. Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model. Journal of King Saud University – Science, v. 34, p. 1-7, 2022.1018-3647https://www.arca.fiocruz.br/handle/icict/5208510.1016/j.jksus.2022.101995engElsevierCompostos FenólicosToxicidadeFarmacologiaMétodosPhenolic CompoundsToxicityPharmacologyMethodsCompuestos FenólicosToxicidadFarmacologíaMétodosComposés PhénoliquesToxicitéPharmacologieMéthodesPharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Regional do Cariri. Laboratório de Bioprospecção do Semiárido. Crato, CE, Brasil.Universidade Regional do Cariri. Laboratório de Bioprospecção do Semiárido. Crato, CE, Brasil.Universidade Federal de Pernambuco. Programa de Pós-Graduação em Ciências Biológicas. Recife, PE, CE, Brasil.Universidade Federal de Pernambuco. Laboratório de Antibióticos. Recife, PE, BrasilUniversidade Regional do Cariri. Laboratório de Bioprospecção do Semiárido. Crato, CE, Brasil.Universidade Regional do Cariri. Laboratório de Simulações e Espectroscopia Molecular. Crato, CE, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisa em Genética e Hematologia Translacional. Salvador, BA, Brasil.Universidade Regional do Cariri. Laboratório de Microbiologia e Biologia Molecular. Crato, CE, Brasil.Ferdows School of Paramedical and Health. Birjand University of Medical Sciences. Birjand, Iran.Department of Clinical Tropical Medicine. Faculty of Tropical Medicine. Mahidol University. Bangkok, Thailand.Universidade Regional do Cariri. Laboratório de Bioprospecção do Semiárido. Crato, CE, Brasil.Universidade Regional do Cariri. Laboratório do Laboratório de Crustáceos do Semi-Árido. Crato, CE, BrasilIntroduction:The rapid emergence of multidrug-resistant bacteria has demanded the discovery of new drugs capable of inhibiting bacterial resistance mechanisms, such as those mediated by efflux proteins and β-lactamases. Objective: Considering the evidence indicating that phenylpropanoids are effective substances against bacterial resistance, the present study aimed to evaluate the antibacterial activity of compounds allylbenzene and allylanisol against the Staphylococcus aureus strain SA-K4414, which carries genes encoding the β-lactamase and QacA/B proteins. Methods:The Minimum Inhibitory Concentrations (MICs) were determined by the broth microdilution method. The expression of β-lactamase was verified through the association of the inhibitor sulbactam with the antibiotic ampicillin, while its inhibition was analyzed through the reduction of the penicillin MIC in the presence of phenolic compounds. The efflux pump inhibition was analyzed by the reduction in the MIC of the pump substrate ethidium bromide. The toxicity against Drosophila melanogaster was assessed by evaluating mortality and negative geotaxis through the fumigation method. Results: The results demonstrated that the phenylpropanoids failed to show relevant direct antibacterial activity, with MIC values above 1024 µg/mL. The association of allylbenzene and allylanisole with penicillin potentiated its antibacterial effect, reducing the antibiotic MIC (512 µg/mL) to 128 µg/mL and 256 µg/mL, respectively. The compounds also reduced the MIC of EtBr, indicating a possible inhibitory effect against the β-lactamase enzyme and the QacA/B efflux protein. Regarding toxicity, allylbenzene (after 12 h) and allylanisole (after 3 h) presented EC50 of 19.21 μL/mL and 11.07 μL/mL, respectively. These compounds also caused damage to the locomotor system of flies, which was potentiated following increasing concentrations and exposure period. Conclusion: It is concluded that the compounds allylbenzene and allylanisole can inhibit the resistance mechanisms mediated by the β-lactamase enzyme and the efflux protein QacA/B. However, due to the significant toxicity observed, further research is required to assess the safety of these compounds.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52085/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLopes, Thais P. - Pharmacological activities.pdfLopes, Thais P. - Pharmacological activities.pdfapplication/pdf569539https://www.arca.fiocruz.br/bitstream/icict/52085/2/Lopes%2c%20Thais%20P.%20-%20Pharmacological%20activities.pdf3e28fc2b1de181e05587ba5bf6a1b5e1MD52icict/520852023-03-15 14:34:34.13oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model |
| title |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model |
| spellingShingle |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model Lopes, Thais Pereira Compostos Fenólicos Toxicidade Farmacologia Métodos Phenolic Compounds Toxicity Pharmacology Methods Compuestos Fenólicos Toxicidad Farmacología Métodos Composés Phénoliques Toxicité Pharmacologie Méthodes |
| title_short |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model |
| title_full |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model |
| title_fullStr |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model |
| title_full_unstemmed |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model |
| title_sort |
Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model |
| author |
Lopes, Thais Pereira |
| author_facet |
Lopes, Thais Pereira Barbosa, Cristina Rodrigues dos Santos Macêdo, Nair Silva Scherf, Jackelyne Roberta Santos, Maria Apoliana Costa dos Freitas, Thiago Sampaio de Ribeiro Filho, Jaime Coutinho, Henrique Douglas Melo Siyadatpanah, Abolghasem Wilairatana, Polrat Cunha, Francisco Assis Bezerra da Pinheiro, Alysson Pontes |
| author_role |
author |
| author2 |
Barbosa, Cristina Rodrigues dos Santos Macêdo, Nair Silva Scherf, Jackelyne Roberta Santos, Maria Apoliana Costa dos Freitas, Thiago Sampaio de Ribeiro Filho, Jaime Coutinho, Henrique Douglas Melo Siyadatpanah, Abolghasem Wilairatana, Polrat Cunha, Francisco Assis Bezerra da Pinheiro, Alysson Pontes |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lopes, Thais Pereira Barbosa, Cristina Rodrigues dos Santos Macêdo, Nair Silva Scherf, Jackelyne Roberta Santos, Maria Apoliana Costa dos Freitas, Thiago Sampaio de Ribeiro Filho, Jaime Coutinho, Henrique Douglas Melo Siyadatpanah, Abolghasem Wilairatana, Polrat Cunha, Francisco Assis Bezerra da Pinheiro, Alysson Pontes |
| dc.subject.other.pt_BR.fl_str_mv |
Compostos Fenólicos Toxicidade Farmacologia Métodos |
| topic |
Compostos Fenólicos Toxicidade Farmacologia Métodos Phenolic Compounds Toxicity Pharmacology Methods Compuestos Fenólicos Toxicidad Farmacología Métodos Composés Phénoliques Toxicité Pharmacologie Méthodes |
| dc.subject.en.pt_BR.fl_str_mv |
Phenolic Compounds Toxicity Pharmacology Methods |
| dc.subject.es.pt_BR.fl_str_mv |
Compuestos Fenólicos Toxicidad Farmacología Métodos |
| dc.subject.fr.pt_BR.fl_str_mv |
Composés Phénoliques Toxicité Pharmacologie Méthodes |
| description |
Universidade Regional do Cariri. Laboratório de Bioprospecção do Semiárido. Crato, CE, Brasil. |
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2022 |
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2022-04-07T14:26:25Z |
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2022-04-07T14:26:25Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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LOPES, Thais Pereira et al. Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model. Journal of King Saud University – Science, v. 34, p. 1-7, 2022. |
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https://www.arca.fiocruz.br/handle/icict/52085 |
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1018-3647 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.jksus.2022.101995 |
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LOPES, Thais Pereira et al. Pharmacological activities of allylbenzene and allylanisole phenylpropanoids: inhibition of antibiotic resistance targets and toxicity profile in a drosophila melanogaster model. Journal of King Saud University – Science, v. 34, p. 1-7, 2022. 1018-3647 10.1016/j.jksus.2022.101995 |
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Elsevier |
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Elsevier |
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