Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata

Bibliographic Details
Main Author: Figueiredo-Carvalho, Maria Helena Galdino
Publication Date: 2017
Other Authors: Ramos, Lívia de Souza, Barbedo, Leonardo Silva, Oliveira, Jean Carlos Almeida de, Santos, André Luis Souza dos, Almeida-Paes, Rodrigo, Zancopé-Oliveira, Rosely Maria
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/31677
Summary: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Figueiredo-Carvalho, Maria Helena GaldinoRamos, Lívia de SouzaBarbedo, Leonardo SilvaOliveira, Jean Carlos Almeida deSantos, André Luis Souza dosAlmeida-Paes, RodrigoZancopé-Oliveira, Rosely Maria2019-02-15T13:49:05Z2019-02-15T13:49:05Z2017FIGUEIREDO-CARVALHO, Maria Helena Galdino et al. Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata. Mediators of Inflammation, v. 2017, 1-10, 2017.0962-9351https://www.arca.fiocruz.br/handle/icict/3167710.1155/2017/89528782314-6141engHindawi Publishing CorporationRelationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabratainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Candida glabrata is a facultative intracellular opportunistic fungal pathogen in human infections. Several virulence-associated attributes are involved in its pathogenesis, host-pathogen interactions, modulation of host immune defenses, and regulation of antifungal drug resistance. This study evaluated the in vitro antifungal susceptibility profile to five antifungal agents, the production of seven hydrolytic enzymes related to virulence, and the relationship between these phenotypes in 91 clinical strains of C. glabrata. All C. glabrata strains were susceptible to flucytosine. However, some of these strains showed resistance to amphotericin B (9.9%), fluconazole (15.4%), itraconazole (5.5%), or micafungin (15.4%). Overall, C. glabrata strains were good producers of catalase, aspartic protease, esterase, phytase, and hemolysin. However, caseinase and phospholipase in vitro activities were not detected. Statistically significant correlations were identified between micafungin minimum inhibitory concentration (MIC) and esterase production, between fluconazole and micafungin MIC and hemolytic activity, and between amphotericin B MIC and phytase production. These results contribute to clarify some of the C. glabrata mechanisms of pathogenicity. Moreover, the association between some virulence attributes and the regulation of antifungal resistance encourage the development of new therapeutic strategies involving virulence mechanisms as potential targets for effective antifungal drug development for the treatment of C. glabrata infections.Antifungal susceptibilityVirulenceHydrolytic enzymesCandida glabrataBrazilian clinical strainsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
title Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
spellingShingle Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
Figueiredo-Carvalho, Maria Helena Galdino
Antifungal susceptibility
Virulence
Hydrolytic enzymes
Candida glabrata
Brazilian clinical strains
title_short Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
title_full Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
title_fullStr Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
title_full_unstemmed Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
title_sort Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata
author Figueiredo-Carvalho, Maria Helena Galdino
author_facet Figueiredo-Carvalho, Maria Helena Galdino
Ramos, Lívia de Souza
Barbedo, Leonardo Silva
Oliveira, Jean Carlos Almeida de
Santos, André Luis Souza dos
Almeida-Paes, Rodrigo
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Ramos, Lívia de Souza
Barbedo, Leonardo Silva
Oliveira, Jean Carlos Almeida de
Santos, André Luis Souza dos
Almeida-Paes, Rodrigo
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Figueiredo-Carvalho, Maria Helena Galdino
Ramos, Lívia de Souza
Barbedo, Leonardo Silva
Oliveira, Jean Carlos Almeida de
Santos, André Luis Souza dos
Almeida-Paes, Rodrigo
Zancopé-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Antifungal susceptibility
Virulence
Hydrolytic enzymes
Candida glabrata
Brazilian clinical strains
topic Antifungal susceptibility
Virulence
Hydrolytic enzymes
Candida glabrata
Brazilian clinical strains
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2019-02-15T13:49:05Z
dc.date.available.fl_str_mv 2019-02-15T13:49:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FIGUEIREDO-CARVALHO, Maria Helena Galdino et al. Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata. Mediators of Inflammation, v. 2017, 1-10, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31677
dc.identifier.issn.pt_BR.fl_str_mv 0962-9351
dc.identifier.doi.none.fl_str_mv 10.1155/2017/8952878
dc.identifier.eissn.none.fl_str_mv 2314-6141
identifier_str_mv FIGUEIREDO-CARVALHO, Maria Helena Galdino et al. Relationship between the antifungal susceptibility profile and the production of virulence-related hydrolytic enzymes in Brazilian clinical strains of Candida glabrata. Mediators of Inflammation, v. 2017, 1-10, 2017.
0962-9351
10.1155/2017/8952878
2314-6141
url https://www.arca.fiocruz.br/handle/icict/31677
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31677/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31677/2/ve_Figueiredo-Carvalho_Maria_etal_INI_2017.pdf
https://www.arca.fiocruz.br/bitstream/icict/31677/3/ve_Figueiredo-Carvalho_Maria_etal_INI_2017.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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