Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40804 |
Resumo: | Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. |
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Machado, Laís FernandaSanfelice, Raquel ArrudaBosqui, Larissa RodriguesAssolini, João PauloScandorieiro, SaraNavarro, Italmar TeodoricoCataneo, Allan Henrique DepieriWowk, Pryscilla FaniniNakazato, GersonBordignon, JulianoPavanelli, Wander RogerioConchon-Costa, IveteCosta, Idessania Nazareth2020-04-16T15:00:12Z2020-04-16T15:00:12Z2020MACHADO, Laís Fernanda. et al. Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction. Experimental Parasitology, Rio de Janeiro, n. 211, p. 1-9, 2020.0014-4894https://www.arca.fiocruz.br/handle/icict/4080410.1016/j.exppara.2020.107853porElsevierBiogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators inductioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Microbiologia. Laboratório de Bacteriologia Básica e Aplicada. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Medicina Veterinária Preventiva. Laboratório de Zoonoses e Saúde Pública. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento de Microbiologia. Laboratório de Bacteriologia Básica e Aplicada. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.The highlights of biogenic silver nanoparticles (AgNp-Bio) include low toxicity - depending on size and concentration - and efficient antiparasitic activity. Therefore, the objective of this study was to assess the action of the AgNp-Bio on HeLa cells in an infection with strain of RH Toxoplasma gondii. Firstly, we performed a cellular viability test and characterized the AgNp-Bio to proceed with the infection of HeLa cells with T. gondii to be treated using AgNp-Bio or conventional drugs. Subsequently, we determined the level of standard cytokines Th1/Th2 as well as the content of nitric oxide (NO) and reactive oxygen species (ROS). Results indicated a mean size of 69 nm in diameter for the AgNp-Bio and obtained a dose-dependent toxicity. In addition, the concentrations of 3 and 6 μM promoted a significant decrease in adherence, infection, and intracellular proliferation. We also found lower IL-8 and production of inflammatory mediators. Thus, the nanoparticles reduced the adherence, infection, and proliferation of ROS and NO, in addition to immunomodulating the IL-8. Therefore, our data proved relevant to introduce a promising therapeutic alternative to toxoplasmosis.ToxoplasmosistherapyHeLa CellsNanoparticlesToxoplasmoseterapiaCélulas HeLaNanopartículasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40804/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1-s2.0-S001448.pdf1-s2.0-S001448.pdfapplication/pdf2278022https://www.arca.fiocruz.br/bitstream/icict/40804/2/1-s2.0-S001448.pdf617f09679791e2eedbca327017b63564MD52TEXT1-s2.0-S001448.pdf.txt1-s2.0-S001448.pdf.txtExtracted texttext/plain53902https://www.arca.fiocruz.br/bitstream/icict/40804/3/1-s2.0-S001448.pdf.txt7e5fd254e07f04f5c692fb6bb87af618MD53icict/408042020-04-17 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| dc.title.pt_BR.fl_str_mv |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction |
| title |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction |
| spellingShingle |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction Machado, Laís Fernanda Toxoplasmosis therapy HeLa Cells Nanoparticles Toxoplasmose terapia Células HeLa Nanopartículas |
| title_short |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction |
| title_full |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction |
| title_fullStr |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction |
| title_full_unstemmed |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction |
| title_sort |
Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction |
| author |
Machado, Laís Fernanda |
| author_facet |
Machado, Laís Fernanda Sanfelice, Raquel Arruda Bosqui, Larissa Rodrigues Assolini, João Paulo Scandorieiro, Sara Navarro, Italmar Teodorico Cataneo, Allan Henrique Depieri Wowk, Pryscilla Fanini Nakazato, Gerson Bordignon, Juliano Pavanelli, Wander Rogerio Conchon-Costa, Ivete Costa, Idessania Nazareth |
| author_role |
author |
| author2 |
Sanfelice, Raquel Arruda Bosqui, Larissa Rodrigues Assolini, João Paulo Scandorieiro, Sara Navarro, Italmar Teodorico Cataneo, Allan Henrique Depieri Wowk, Pryscilla Fanini Nakazato, Gerson Bordignon, Juliano Pavanelli, Wander Rogerio Conchon-Costa, Ivete Costa, Idessania Nazareth |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Machado, Laís Fernanda Sanfelice, Raquel Arruda Bosqui, Larissa Rodrigues Assolini, João Paulo Scandorieiro, Sara Navarro, Italmar Teodorico Cataneo, Allan Henrique Depieri Wowk, Pryscilla Fanini Nakazato, Gerson Bordignon, Juliano Pavanelli, Wander Rogerio Conchon-Costa, Ivete Costa, Idessania Nazareth |
| dc.subject.en.pt_BR.fl_str_mv |
Toxoplasmosis therapy HeLa Cells Nanoparticles |
| topic |
Toxoplasmosis therapy HeLa Cells Nanoparticles Toxoplasmose terapia Células HeLa Nanopartículas |
| dc.subject.decs.pt_BR.fl_str_mv |
Toxoplasmose terapia Células HeLa Nanopartículas |
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Universidade Estadual de Londrina. Departamento de Patologia Experimental. Laboratório de Imunoparasitologia das Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. |
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2020 |
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2020-04-16T15:00:12Z |
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2020-04-16T15:00:12Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MACHADO, Laís Fernanda. et al. Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction. Experimental Parasitology, Rio de Janeiro, n. 211, p. 1-9, 2020. |
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https://www.arca.fiocruz.br/handle/icict/40804 |
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0014-4894 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.exppara.2020.107853 |
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MACHADO, Laís Fernanda. et al. Biogenic silver nanoparticles reduce adherence, infection, and proliferation of toxoplasma gondii RH strain in HeLa cells without inflammatory mediators induction. Experimental Parasitology, Rio de Janeiro, n. 211, p. 1-9, 2020. 0014-4894 10.1016/j.exppara.2020.107853 |
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Elsevier |
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