Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23409 |
Resumo: | Universidade Federal de Pernambuco. Departamento de Genética. Laboratório de Estudos Moleculares e Terapia Experimenta. Recife, PE, Brasil. |
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Lima, E. G.Lira, R. C.Jesus, A. L. S.Dhalia, R.Freitas, A. C.2017-11-28T13:06:09Z2017-11-28T13:06:09Z2014LIMA, E. G. et al. Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene. Genetics and molecular research: GMR, v. 13, n. 1, p. 1121–1126, 20 fev. 2014.1676-5680https://www.arca.fiocruz.br/handle/icict/2340910.4238/2014.February.20.13engGenetics and Molecular ResearchPapillomavirus BovinoVacinas de DNABPVBovine papillomavirusDNA vaccineBPVProteínas do CapsídeogenéticaProteínas Oncogênicas ViraismetabolismoCélulas HEK293AnimaisPapillomavirus Bovino 1fisiologiaBovinosvirologiaVetores GenéticosHumanosInfecções por Papillomavirusprevenção & controleInfecções por PapillomavirusterapiaInfecções por PapillomavirusveterináriaDevelopment of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Pernambuco. Departamento de Genética. Laboratório de Estudos Moleculares e Terapia Experimenta. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Genética. Laboratório de Estudos Moleculares e Terapia Experimenta. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Genética. Laboratório de Estudos Moleculares e Terapia Experimenta. Recife, PE, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Genética. Laboratório de Estudos Moleculares e Terapia Experimenta. Recife, PE, Brasil.Papillomaviruses are known to cause tumor lesions, generally benign, in epithelial tissues of diverse organisms; these lesions may progress to cancer under suitable conditions. Bovine papillomavirus (BPV) can cause urinary bladder cancer and cancer of the upper gastrointestinal tract. Furthermore, BPV1 and BPV2 are implicated in the development of tumors in equids. Many studies with animal models clearly demonstrate that DNA vaccines are very effective tools in controlling viral infections, providing strong humoral and cellular immune responses. In this study, we have described the development of two vaccine constructs for the control of diseases caused by BPV. The 1st strategy is prophylactic and is based on the L2 gene; the 2nd is therapeutic and is based on the E5 gene. Vaccine constructs were obtained and evaluated in vitro in mammalian cells. The results show the occurrence of E5 and L2 transcription and viral protein production. These results confirm the functionality of the vaccine constructs in mammalian cells. This is the 1st step in the development of a DNA-based vaccine strategy for the control and/or treatment of diseases caused by BPV.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23409/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALART. Development of a DNA-based - LIMA.pdfART. 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| dc.title.pt_BR.fl_str_mv |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene |
| title |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene |
| spellingShingle |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene Lima, E. G. Papillomavirus Bovino Vacinas de DNA BPV Bovine papillomavirus DNA vaccine BPV Proteínas do Capsídeo genética Proteínas Oncogênicas Virais metabolismo Células HEK293 Animais Papillomavirus Bovino 1 fisiologia Bovinos virologia Vetores Genéticos Humanos Infecções por Papillomavirus prevenção & controle Infecções por Papillomavirus terapia Infecções por Papillomavirus veterinária |
| title_short |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene |
| title_full |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene |
| title_fullStr |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene |
| title_full_unstemmed |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene |
| title_sort |
Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene |
| author |
Lima, E. G. |
| author_facet |
Lima, E. G. Lira, R. C. Jesus, A. L. S. Dhalia, R. Freitas, A. C. |
| author_role |
author |
| author2 |
Lira, R. C. Jesus, A. L. S. Dhalia, R. Freitas, A. C. |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Lima, E. G. Lira, R. C. Jesus, A. L. S. Dhalia, R. Freitas, A. C. |
| dc.subject.other.pt_BR.fl_str_mv |
Papillomavirus Bovino Vacinas de DNA BPV |
| topic |
Papillomavirus Bovino Vacinas de DNA BPV Bovine papillomavirus DNA vaccine BPV Proteínas do Capsídeo genética Proteínas Oncogênicas Virais metabolismo Células HEK293 Animais Papillomavirus Bovino 1 fisiologia Bovinos virologia Vetores Genéticos Humanos Infecções por Papillomavirus prevenção & controle Infecções por Papillomavirus terapia Infecções por Papillomavirus veterinária |
| dc.subject.en.pt_BR.fl_str_mv |
Bovine papillomavirus DNA vaccine BPV |
| dc.subject.decs.pt_BR.fl_str_mv |
Proteínas do Capsídeo genética Proteínas Oncogênicas Virais metabolismo Células HEK293 Animais Papillomavirus Bovino 1 fisiologia Bovinos virologia Vetores Genéticos Humanos Infecções por Papillomavirus prevenção & controle Infecções por Papillomavirus terapia Infecções por Papillomavirus veterinária |
| description |
Universidade Federal de Pernambuco. Departamento de Genética. Laboratório de Estudos Moleculares e Terapia Experimenta. Recife, PE, Brasil. |
| publishDate |
2014 |
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2014 |
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2017-11-28T13:06:09Z |
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2017-11-28T13:06:09Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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LIMA, E. G. et al. Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene. Genetics and molecular research: GMR, v. 13, n. 1, p. 1121–1126, 20 fev. 2014. |
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https://www.arca.fiocruz.br/handle/icict/23409 |
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1676-5680 |
| dc.identifier.doi.none.fl_str_mv |
10.4238/2014.February.20.13 |
| identifier_str_mv |
LIMA, E. G. et al. Development of a DNA-based vaccine strategy against bovine papillomavirus infection, involving the E5 or L2 gene. Genetics and molecular research: GMR, v. 13, n. 1, p. 1121–1126, 20 fev. 2014. 1676-5680 10.4238/2014.February.20.13 |
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Genetics and Molecular Research |
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Genetics and Molecular Research |
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