Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms

Detalhes bibliográficos
Autor(a) principal: Fabri, Rodrigo Luiz
Data de Publicação: 2014
Outros Autores: Aragão, Danielle Maria de Oliveira, Florêncio, Jônatas Rodrigues, Pinto, Nícolas de Castro Campos, Mattos, Ana Carolina Alves, Coelho, Paulo Marcos Zech, Castañon, Maria Christina Marques Nogueira, Vasconcelos, Eveline Gomes, Pinto, Priscila de Faria, Scio, Elita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9494
Resumo: Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
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spelling Fabri, Rodrigo LuizAragão, Danielle Maria de OliveiraFlorêncio, Jônatas RodriguesPinto, Nícolas de Castro CamposMattos, Ana Carolina AlvesCoelho, Paulo Marcos ZechCastañon, Maria Christina Marques NogueiraVasconcelos, Eveline GomesPinto, Priscila de FariaScio, Elita2015-02-13T12:32:32Z2015-02-13T12:32:32Z2014FABRI, Rodrigo Luiz et al. Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms. BioMed Research International, 2014, Article ID 941318.2314-6141https://www.arca.fiocruz.br/handle/icict/949410.1155/2014/941318engHindawi Pub. Co.Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Wormsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, BrazilFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, BrazilFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, BrazilFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, BrazilFundação Oswaldo Cruz. Rene Rachou Research Center. Schistosomiasis Laboratory. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Rene Rachou Research Center. Schistosomiasis Laboratory. Belo Horizonte, MG, BrazilFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Morphology. Juiz de Fora, MG, BrazilFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Protein Structure and Function Study Laboratory. Juiz de Fora, MG, BrazilFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Protein Structure and Function Study Laboratory. Juiz de Fora, MG, BrazilFederal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, BrazilThe aims of this work were to evaluate the in vitro and in vivo schistosomicidal properties of the methanolic extract of the aerial parts of Mitracarpus frigidus (MFM) and to determine its HPLC profile. For the in vitro experiment, four pairs of adult worms, obtained from infected mice, were exposed to different concentrations of MFM (100 to 400 g/mL) for 24 and 48 h and analyzed under an inverted microscope. For the in vivo experiment, mice were inoculated with cercariae and, 20 days after infection, MFM (100 and 300mg/kg) was administered orally for the following 25 days. Mice were euthanized after 60 days. MFM showed in vitro schistosomicidal activity, exhibiting the opening of the gynaecophoral canal of some male schistosomes, the presence of contorted muscles, vesicles, and the darkening of the paired worms skin. In vivo experiments showed that MFM treatments significantly reduced total wormcount, as praziquantel, showing a decrease in liver and spleen weight. Also, a significant reduction in granuloma density was observed. MFM treatment did not cause alterations in the liver function of either infected or noninfected mice. The HPLC chromatogram profile showed the presence of kaempferol-O-rutinoside, rutin, kaempferol, psychorubrin, and ursolic acid.ChromatographyHigh Pressure Liquid/methodsPlant Extracts/chemistrySchistosoma mansoni/drug effectsSpleen/drug effectsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9494/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_075.pdf2014_075.pdfapplication/pdf2903719https://www.arca.fiocruz.br/bitstream/icict/9494/2/2014_075.pdf5cdf205ca2a1268dea66bc0fc2a7ce89MD52TEXT2014_075.pdf.txt2014_075.pdf.txtExtracted texttext/plain43554https://www.arca.fiocruz.br/bitstream/icict/9494/3/2014_075.pdf.txt16a3bb3e83373160502e6c089a5532b0MD53icict/94942019-06-19 10:07:31.523oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:31Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
title Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
spellingShingle Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
Fabri, Rodrigo Luiz
Chromatography
High Pressure Liquid/methods
Plant Extracts/chemistry
Schistosoma mansoni/drug effects
Spleen/drug effects
title_short Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
title_full Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
title_fullStr Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
title_full_unstemmed Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
title_sort Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
author Fabri, Rodrigo Luiz
author_facet Fabri, Rodrigo Luiz
Aragão, Danielle Maria de Oliveira
Florêncio, Jônatas Rodrigues
Pinto, Nícolas de Castro Campos
Mattos, Ana Carolina Alves
Coelho, Paulo Marcos Zech
Castañon, Maria Christina Marques Nogueira
Vasconcelos, Eveline Gomes
Pinto, Priscila de Faria
Scio, Elita
author_role author
author2 Aragão, Danielle Maria de Oliveira
Florêncio, Jônatas Rodrigues
Pinto, Nícolas de Castro Campos
Mattos, Ana Carolina Alves
Coelho, Paulo Marcos Zech
Castañon, Maria Christina Marques Nogueira
Vasconcelos, Eveline Gomes
Pinto, Priscila de Faria
Scio, Elita
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fabri, Rodrigo Luiz
Aragão, Danielle Maria de Oliveira
Florêncio, Jônatas Rodrigues
Pinto, Nícolas de Castro Campos
Mattos, Ana Carolina Alves
Coelho, Paulo Marcos Zech
Castañon, Maria Christina Marques Nogueira
Vasconcelos, Eveline Gomes
Pinto, Priscila de Faria
Scio, Elita
dc.subject.en.pt_BR.fl_str_mv Chromatography
High Pressure Liquid/methods
Plant Extracts/chemistry
Schistosoma mansoni/drug effects
Spleen/drug effects
topic Chromatography
High Pressure Liquid/methods
Plant Extracts/chemistry
Schistosoma mansoni/drug effects
Spleen/drug effects
description Federal University of Juiz de Fora. Institute of Biological Sciences. Department of Biochemistry. Bioactive Natural Products Laboratory. Juiz de Fora, MG, Brazil
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-02-13T12:32:32Z
dc.date.available.fl_str_mv 2015-02-13T12:32:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FABRI, Rodrigo Luiz et al. Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms. BioMed Research International, 2014, Article ID 941318.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9494
dc.identifier.issn.none.fl_str_mv 2314-6141
dc.identifier.doi.none.fl_str_mv 10.1155/2014/941318
identifier_str_mv FABRI, Rodrigo Luiz et al. Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms. BioMed Research International, 2014, Article ID 941318.
2314-6141
10.1155/2014/941318
url https://www.arca.fiocruz.br/handle/icict/9494
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Pub. Co.
publisher.none.fl_str_mv Hindawi Pub. Co.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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