Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors

Detalhes bibliográficos
Autor(a) principal: Rodart, Itatiana F.
Data de Publicação: 2021
Outros Autores: Pares, Madalena M., Mendes, Aline, Accardo, Camila M., Martins, João R. M., Silva, Cleidenice B., Carvalho, Fabrício O., Barreto, José A., Reis, Mitermayer Galvão dos, Tersariol, Ivarne L. S., Nader, Helena B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49136
Resumo: São Paulo Research Foundation-FAPESP (http://www. fapesp.br, Accessed on 30 April 2021) Grants 2015/03964-6; awarded to H.B.N. and I.L.S.T.; I.F.R. and C.M.A. were recipients of scholarship grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, CAPES (http://www.capes.gov.br/, Accessed on 30 April 2021); H.B.N. and I.L.S.T. are Productivity Fellows from Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, CNPq (https://www.gov.br/cnpq/pt-br, Accessed on 30 April 2021).
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spelling Rodart, Itatiana F.Pares, Madalena M.Mendes, AlineAccardo, Camila M.Martins, João R. M.Silva, Cleidenice B.Carvalho, Fabrício O.Barreto, José A.Reis, Mitermayer Galvão dosTersariol, Ivarne L. S.Nader, Helena B.2021-09-22T18:49:54Z2021-09-22T18:49:54Z2021RODART, Itatiana F. et al. Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors. Molecules, 2021.1420-3049https://www.arca.fiocruz.br/handle/icict/4913610.3390/molecules26133892São Paulo Research Foundation-FAPESP (http://www. fapesp.br, Accessed on 30 April 2021) Grants 2015/03964-6; awarded to H.B.N. and I.L.S.T.; I.F.R. and C.M.A. were recipients of scholarship grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, CAPES (http://www.capes.gov.br/, Accessed on 30 April 2021); H.B.N. and I.L.S.T. are Productivity Fellows from Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, CNPq (https://www.gov.br/cnpq/pt-br, Accessed on 30 April 2021).Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil.Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Bioquímica. São Paulo, SP, Brazil.Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil.Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil.Associação Beneficente de Coleta de Sangue. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Hepatites Virais. Salvador, BA, Brasil.Background: The disease caused by hepatitis C virus (HCV) is asymptomatic, silent, and progressive liver disease. In HCV-infected patients the increase in serum HA is associated with the development of hepatic fibrosis and disease progression. Methods: HCV-RNA detection was performed in all serological samples of blood donors that tested positive using HCV Ultra ELISA. Determination of hyaluronan (HA) was performed in positive HCV samples using ELISA-like fluorometric method. The HA content was compared to HCV viral load, genotype of the virus, liver fibrosis as well as ALT and GGT liver biomarkers. Results: Persistently normal ALT (<40 U/L) and GGT (<50 U/L) serum levels were detected in 75% and 69% of the HCV-Infected blood donors, respectively. Based on ROC analysis, the HA value < 34.2 ng/mL is an optimal cut-off point to exclude HCV viremia (specificity = 91%, NPV = 99%). Applying HA value 34.2 ng/mL significant liver fibrosis ( F2) can be estimated in 46% of the HCV-infected blood donors. HA serum level ( 34.2 ng/mL) associated with a high ALT level (>40 U/mL) can correctly identify HCV infection and probable liver fibrosis (sensitivity = 96% and specificity = 90%) in asymptomatic blood donors. Conclusions: A high level of HA ( 34.2 ng/mL) in association with ALT ( 40 U/L) in serum can provide a good clinical opportunity to detect HCV-infected asymptomatic persons that potentially require a liver biopsy confirmation and antiviral treatment to prevent the development of advanced liver fibrosis or cirrhosis.engMDPIRNAProgressão da DoençaFibroseFígadoInfecçãoDoadores de sangueSerum hyaluronanDiagnostic accuracyLiver fibrosisLiver enzymesHCV infectionBlood donorsDiagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/49136/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALRodart, Itatiana F Diagnostic.pdfRodart, Itatiana F Diagnostic.pdfapplication/pdf1001546https://www.arca.fiocruz.br/bitstream/icict/49136/2/Rodart%2c%20Itatiana%20F%20Diagnostic.pdfddd6b557bab1442698f28ce0a115daf6MD52icict/491362023-03-15 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dc.title.pt_BR.fl_str_mv Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
title Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
spellingShingle Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
Rodart, Itatiana F.
RNA
Progressão da Doença
Fibrose
Fígado
Infecção
Doadores de sangue
Serum hyaluronan
Diagnostic accuracy
Liver fibrosis
Liver enzymes
HCV infection
Blood donors
title_short Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
title_full Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
title_fullStr Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
title_full_unstemmed Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
title_sort Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors
author Rodart, Itatiana F.
author_facet Rodart, Itatiana F.
Pares, Madalena M.
Mendes, Aline
Accardo, Camila M.
Martins, João R. M.
Silva, Cleidenice B.
Carvalho, Fabrício O.
Barreto, José A.
Reis, Mitermayer Galvão dos
Tersariol, Ivarne L. S.
Nader, Helena B.
author_role author
author2 Pares, Madalena M.
Mendes, Aline
Accardo, Camila M.
Martins, João R. M.
Silva, Cleidenice B.
Carvalho, Fabrício O.
Barreto, José A.
Reis, Mitermayer Galvão dos
Tersariol, Ivarne L. S.
Nader, Helena B.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodart, Itatiana F.
Pares, Madalena M.
Mendes, Aline
Accardo, Camila M.
Martins, João R. M.
Silva, Cleidenice B.
Carvalho, Fabrício O.
Barreto, José A.
Reis, Mitermayer Galvão dos
Tersariol, Ivarne L. S.
Nader, Helena B.
dc.subject.other.pt_BR.fl_str_mv RNA
Progressão da Doença
Fibrose
Fígado
Infecção
Doadores de sangue
topic RNA
Progressão da Doença
Fibrose
Fígado
Infecção
Doadores de sangue
Serum hyaluronan
Diagnostic accuracy
Liver fibrosis
Liver enzymes
HCV infection
Blood donors
dc.subject.en.pt_BR.fl_str_mv Serum hyaluronan
Diagnostic accuracy
Liver fibrosis
Liver enzymes
HCV infection
Blood donors
description São Paulo Research Foundation-FAPESP (http://www. fapesp.br, Accessed on 30 April 2021) Grants 2015/03964-6; awarded to H.B.N. and I.L.S.T.; I.F.R. and C.M.A. were recipients of scholarship grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, CAPES (http://www.capes.gov.br/, Accessed on 30 April 2021); H.B.N. and I.L.S.T. are Productivity Fellows from Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, CNPq (https://www.gov.br/cnpq/pt-br, Accessed on 30 April 2021).
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-09-22T18:49:54Z
dc.date.available.fl_str_mv 2021-09-22T18:49:54Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODART, Itatiana F. et al. Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors. Molecules, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49136
dc.identifier.issn.pt_BR.fl_str_mv 1420-3049
dc.identifier.doi.none.fl_str_mv 10.3390/molecules26133892
identifier_str_mv RODART, Itatiana F. et al. Diagnostic Accuracy of Serum Hyaluronan for Detecting HCV Infection and Liver Fibrosis in Asymptomatic Blood Donors. Molecules, 2021.
1420-3049
10.3390/molecules26133892
url https://www.arca.fiocruz.br/handle/icict/49136
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49136/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/49136/2/Rodart%2c%20Itatiana%20F%20Diagnostic.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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