ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway

Detalhes bibliográficos
Autor(a) principal: Moraes, Lais
Data de Publicação: 2017
Outros Autores: Zanchin, Nilson Ivo Tonin, Cerutti, Janete M.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24948
Resumo: Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Morfologia e Genética. Divisão de Genética. Laboratório de Bases Genéticas de Tumores de Tiróide. São Paulo, SP, Brasil.
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spelling Moraes, LaisZanchin, Nilson Ivo ToninCerutti, Janete M.2018-02-26T18:14:00Z2018-02-26T18:14:00Z2017MORAES, Lais; ZANCHIN, Nilson Ivo Tonin; CERUTTI, Janete M. ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway. Oncotarget, v. 8, n. 40, p. 67769-67781, 2017.1949-2553https://www.arca.fiocruz.br/handle/icict/2494810.18632/oncotarget.18840porImpact JournalsPI3K/AKTAdenocarcinomaFollicularWAVE2 ProteinPhosphoproteinsThyroid NeoplasmsAdenocarcinoma FolicularFosfoproteínasNeoplasias de la TiroidesAdenocarcinoma FolicularFosfoproteínasNeoplasias da Glândula TireoideABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathwayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Morfologia e Genética. Divisão de Genética. Laboratório de Bases Genéticas de Tumores de Tiróide. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Morfologia e Genética. Divisão de Genética. Laboratório de Bases Genéticas de Tumores de Tiróide. São Paulo, SP, Brasil.We previously reported that ABI3 expression is lost in follicular thyroid carcinomas and its restoration significantly inhibited cell growth, invasiveness, migration, and reduced tumor growth in vivo. The mechanistic basis by which ABI3 exerts its tumor suppressive effects is not fully understood. In this study, we show that ABI3 is a phosphoprotein. Using proteomic array analysis, we showed that ABI3 modulated distinct cancer-related pathways in thyroid cancer cells. The KEA analysis found that PI3K substrates were enriched and forced expression of ABI3 markedly decreased the phosphorylation of AKT and the downstream-targeted protein pGSK3β. We next used immunoprecipitation combined with mass spectrometry to identify ABI3-interacting proteins that may be involved in modulating/integrating signaling pathways. We identified 37 ABI3 partners, including several components of the canonical WAVE regulatory complex (WRC) such as WAVE2/CYF1P1/NAP1, suggesting that ABI3 function might be regulated through WRC. Both, pharmacological inhibition of the PI3K/AKT pathway and mutation at residue S342 of ABI3, which is predicted to be phosphorylated by AKT, provided evidences that the non-phosphorylated form of ABI3 is preferentially present in the WRC protein complex. Collectively, our findings suggest that ABI3 might be a downstream mediator of the PI3K/AKT pathway that might disrupt WRC via ABI3 phosphorylation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/24948/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALZanchinABI3a.pdfZanchinABI3a.pdfapplication/pdf3835908https://www.arca.fiocruz.br/bitstream/icict/24948/2/ZanchinABI3a.pdf66017a440381ac7934b77b7ee800b155MD52TEXTZanchinABI3a.pdf.txtZanchinABI3a.pdf.txtExtracted texttext/plain47775https://www.arca.fiocruz.br/bitstream/icict/24948/3/ZanchinABI3a.pdf.txt44ced41aaba617148424072fd895fbf8MD53icict/249482019-12-15 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dc.title.pt_BR.fl_str_mv ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
title ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
spellingShingle ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
Moraes, Lais
PI3K/AKT
Adenocarcinoma
Follicular
WAVE2 Protein
Phosphoproteins
Thyroid Neoplasms
Adenocarcinoma Folicular
Fosfoproteínas
Neoplasias de la Tiroides
Adenocarcinoma Folicular
Fosfoproteínas
Neoplasias da Glândula Tireoide
title_short ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
title_full ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
title_fullStr ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
title_full_unstemmed ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
title_sort ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway
author Moraes, Lais
author_facet Moraes, Lais
Zanchin, Nilson Ivo Tonin
Cerutti, Janete M.
author_role author
author2 Zanchin, Nilson Ivo Tonin
Cerutti, Janete M.
author2_role author
author
dc.contributor.author.fl_str_mv Moraes, Lais
Zanchin, Nilson Ivo Tonin
Cerutti, Janete M.
dc.subject.other.pt_BR.fl_str_mv PI3K/AKT
topic PI3K/AKT
Adenocarcinoma
Follicular
WAVE2 Protein
Phosphoproteins
Thyroid Neoplasms
Adenocarcinoma Folicular
Fosfoproteínas
Neoplasias de la Tiroides
Adenocarcinoma Folicular
Fosfoproteínas
Neoplasias da Glândula Tireoide
dc.subject.en.pt_BR.fl_str_mv Adenocarcinoma
Follicular
WAVE2 Protein
Phosphoproteins
Thyroid Neoplasms
dc.subject.es.pt_BR.fl_str_mv Adenocarcinoma Folicular
Fosfoproteínas
Neoplasias de la Tiroides
dc.subject.decs.pt_BR.fl_str_mv Adenocarcinoma Folicular
Fosfoproteínas
Neoplasias da Glândula Tireoide
description Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Morfologia e Genética. Divisão de Genética. Laboratório de Bases Genéticas de Tumores de Tiróide. São Paulo, SP, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-26T18:14:00Z
dc.date.available.fl_str_mv 2018-02-26T18:14:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAES, Lais; ZANCHIN, Nilson Ivo Tonin; CERUTTI, Janete M. ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway. Oncotarget, v. 8, n. 40, p. 67769-67781, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24948
dc.identifier.issn.pt_BR.fl_str_mv 1949-2553
dc.identifier.doi.none.fl_str_mv 10.18632/oncotarget.18840
identifier_str_mv MORAES, Lais; ZANCHIN, Nilson Ivo Tonin; CERUTTI, Janete M. ABI3, a component of the WAVE2 complex, is potentially regulated by PI3K/AKT pathway. Oncotarget, v. 8, n. 40, p. 67769-67781, 2017.
1949-2553
10.18632/oncotarget.18840
url https://www.arca.fiocruz.br/handle/icict/24948
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Impact Journals
publisher.none.fl_str_mv Impact Journals
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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