Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi

Detalhes bibliográficos
Autor(a) principal: Forrest, David M.
Data de Publicação: 2020
Outros Autores: Batista, Michel, Marchini, Fabrício Klerynton, Tempone, Antonio J., Traub-Csekö, Yara Maria
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44096
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
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spelling Forrest, David M.Batista, MichelMarchini, Fabrício KleryntonTempone, Antonio J.Traub-Csekö, Yara Maria2020-10-21T13:47:28Z2020-10-21T13:47:28Z2020FORREST, David M. et al. Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi. Brazilian Journal of Proteomics. N. 227, p. 1-10, 2020.1874-3919https://www.arca.fiocruz.br/handle/icict/4409610.1016/j.jprot.2020.103902porElsevierPsychodidaePhlebotominaeExosomesVirulence FactorsExosomasFactores de VirulenciaExosomesFacteurs de virulenceLeishmania infantum chagasiExossomosFatores de VirulênciaLeishmania infantumProteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Espectrometria de Massas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Espectrometria de Massas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.Leishmania infantum chagasi is the primary etiological agent of visceral leishmaniasis in Latin America, a lethal disease that afflicts hundreds of thousands of people worldwide every year. Previous studies have shown that the parasite releases microvesicles known as exosomes, which prolong and exacerbate infection in the vertebrate vector. However, little is known of their role in the insect vector, the sand fly Lutzomyia longipalpis. Exosomes were isolated from cultured L. i. chagasi in logarithmic (procyclic) (LOG) and stationary phase (metacyclic-like) (STAT) growth stages, which are the parasite stages found in the vector, and submitted to proteomic analysis. Our studies showed that exosomes from LOG and STAT L. i. chagasi display discrete protein profiles. The presence of approximately 50 known virulence factors was detected, including molecules for immunomodulation and evasion (GP63, EF1α, Oligopeptidase), increased pathogenicity (Casein kinase, KMP-11, Cysteine Peptidase and BiP) and parasite protection (Peroxidoxin). Additionally, the majority of ontological terms were associated with both exosome phases, and no substantial ontological enrichment was observed associated with any of the two exosomal stages. We demonstrated that LOG exosomes show a marked increase in protein number and abundance, including many virulence factors, compared to STAT L. i. chagasi exosomes. Significance: The knowledge of the role of Leishmania exosomes on leishmaniasis opened up a new world of potential and complexity regarding our understanding of the disease. In Brazil the majority of visceral leishmaniasis cases are caused by the parasite Leishmania infantum chagasi and transmitted by the vector Lutzomyia longipalpis. While Leishmania exosomes were found to play an active role in the mammalian host, little is understood about their effects on the sand fly, or how they might impact on the insect infection by the parasite. For this reason, we isolated exosomes from two developmental stages of L. i. chagasi that occur within the insect with a view to identifying and describing the alterations they undergo. We have identified many hundreds of proteins within both exosome phases and have developed a structure by which to examine potential candidates. Our findings regarding the composition of the exosome proteome raise many questions regarding their function and provide compelling evidence that exosomes play an active role in the parasite's development within the sand fly.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/44096/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1-s2.0-S187439192030270.pdf1-s2.0-S187439192030270.pdfapplication/pdf1976015https://www.arca.fiocruz.br/bitstream/icict/44096/2/1-s2.0-S187439192030270.pdf4ce5df8da1e9dbca4b9fc40a7ca4ea29MD52TEXT1-s2.0-S187439192030270.pdf.txt1-s2.0-S187439192030270.pdf.txtExtracted texttext/plain60731https://www.arca.fiocruz.br/bitstream/icict/44096/3/1-s2.0-S187439192030270.pdf.txt7e202b8b26feae6f8915dc1b50ee8b6bMD53icict/440962021-10-05 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dc.title.pt_BR.fl_str_mv Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
title Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
spellingShingle Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
Forrest, David M.
Psychodidae
Phlebotominae
Exosomes
Virulence Factors
Exosomas
Factores de Virulencia
Exosomes
Facteurs de virulence
Leishmania infantum chagasi
Exossomos
Fatores de Virulência
Leishmania infantum
title_short Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
title_full Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
title_fullStr Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
title_full_unstemmed Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
title_sort Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi
author Forrest, David M.
author_facet Forrest, David M.
Batista, Michel
Marchini, Fabrício Klerynton
Tempone, Antonio J.
Traub-Csekö, Yara Maria
author_role author
author2 Batista, Michel
Marchini, Fabrício Klerynton
Tempone, Antonio J.
Traub-Csekö, Yara Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Forrest, David M.
Batista, Michel
Marchini, Fabrício Klerynton
Tempone, Antonio J.
Traub-Csekö, Yara Maria
dc.subject.other.pt_BR.fl_str_mv Psychodidae
Phlebotominae
topic Psychodidae
Phlebotominae
Exosomes
Virulence Factors
Exosomas
Factores de Virulencia
Exosomes
Facteurs de virulence
Leishmania infantum chagasi
Exossomos
Fatores de Virulência
Leishmania infantum
dc.subject.en.pt_BR.fl_str_mv Exosomes
Virulence Factors
dc.subject.es.pt_BR.fl_str_mv Exosomas
Factores de Virulencia
dc.subject.fr.pt_BR.fl_str_mv Exosomes
Facteurs de virulence
dc.subject.decs.pt_BR.fl_str_mv Leishmania infantum chagasi
Exossomos
Fatores de Virulência
Leishmania infantum
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Parasitas e Vetores. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-21T13:47:28Z
dc.date.available.fl_str_mv 2020-10-21T13:47:28Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FORREST, David M. et al. Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi. Brazilian Journal of Proteomics. N. 227, p. 1-10, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44096
dc.identifier.issn.pt_BR.fl_str_mv 1874-3919
dc.identifier.doi.none.fl_str_mv 10.1016/j.jprot.2020.103902
identifier_str_mv FORREST, David M. et al. Proteomic analysis of exosomes derived from procyclic and metacyclic-like cultured Leishmania infantum chagasi. Brazilian Journal of Proteomics. N. 227, p. 1-10, 2020.
1874-3919
10.1016/j.jprot.2020.103902
url https://www.arca.fiocruz.br/handle/icict/44096
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44096/1/license.txt
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