Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis

Detalhes bibliográficos
Autor(a) principal: Santos, Alessandro Marins dos
Data de Publicação: 2022
Outros Autores: Silva, Jackline de Paula Ayres, Antunes, Dina, Moreira, Carlos José de Carvalho, Machado, Marcelo Pelajo, Alfaro, David, Zapata, Agustin G., Bonomo, Adriana Cesar, Savino, Wilson, Meis, Juliana de, Oliveira, Désio Aurélio Farias de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/52335
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.
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spelling Santos, Alessandro Marins dosSilva, Jackline de Paula AyresAntunes, DinaMoreira, Carlos José de CarvalhoMachado, Marcelo PelajoAlfaro, DavidZapata, Agustin G.Bonomo, Adriana CesarSavino, WilsonMeis, Juliana deOliveira, Désio Aurélio Farias de2022-04-18T20:11:36Z2022-04-18T20:11:36Z2022SANTOS, Alessandro Martins dos et al. Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis. Frontiers in Cellular and Infection Microbiology, v. 12, Article 800395, p. 1-12, Mar. 2022.2235-2988https://www.arca.fiocruz.br/handle/icict/5233510.3389/fcimb.2022.800395engFrontiers MediaTrypanosoma cruziInfecção oralMedula ósseaTimoHematopoieseCélulas-tronco hematopoiéticasHematopoiese extramedularFluxo multiparamétricoTrypanosoma cruziOral infectionBone marrowThymusHematopoiesisHematopoietic stem cellsExtramedullary hematopoiesisMultiparametric flowOral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Complutense University of Madrid. Faculty of Biology. Department of Cell Biology. Madrid, Spain.Complutense University of Madrid. Faculty of Biology. Department of Cell Biology. Madrid, Spain.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rede de Pesquisa em Neuroimunomodulação do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rede de Pesquisa em Neuroimunomodulação do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rede de Pesquisa em Neuroimunomodulação do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rede de Pesquisa em Neuroimunomodulação do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.During the acute phase of Chagas disease, Trypanosoma cruzi circulation through the bloodstream leads to high tissue parasitism in the host. In primary lymphoid organs, progenitor cell reduction paralleled transient immunosuppression. Herein we showed that acute oral infection in mice promotes diffuse parasitism in bone marrow cells at 14 and 21 days post-infection (dpi), with perivascular regions, intravascular regions, and regions near the bone being target sites of parasite replication. Phenotypic analysis of hematopoietic differentiation in the bone marrow of infected mice showed that the cell number in the tissue is decreased (lineage-negative and lineage-positive cells). Interestingly, analysis of hematopoietic branching points showed that hematopoietic stem and progenitor cells (HSPCs) were significantly increased at 14 dpi. In addition, the pool of progenitors with stem plasticity (HSC-MPP3), as well as multipotent progenitors (MPPs) such as MPP4, also showed this pattern of increase. In contrast, subsequent progenitors that arise from MPPs, such as common lymphoid progenitors (CLPs), lymphoid-primed MPPs (LMPPs), and myeloid progenitors, were not enhanced; conversely, all presented numeric decline. Annexin V staining revealed that cell death increase in the initial hematopoietic branching point probably is not linked to CLPs and that myeloid progenitors decreased at 14 and 21 dpi. In parallel, our investigation provided clues that myeloid progenitor decrease could be associated with an atypical expression of Sca-1 in this population leading to a remarkable increase on LSK-like cells at 14 dpi within the HSPC compartment. Finally, these results led us to investigate HSPC presence in the spleen as a phenomenon triggered during emergency hematopoiesis due to mobilization or expansion of these cells in extramedullary sites. Splenocyte analysis showed a progressive increase in HSPCs between 14 and 21 dpi. Altogether, our study shows that the bone marrow is a target tissue in T. cruzi orally infected mice, leading to a hematopoietic disturbance with LSK-like cell bias accounting on HSPCs possibly affecting myeloid progenitor numbers. The LMPP and CLP reduction converges with defective thymocyte development. Lastly, it is tempting to speculate that the extramedullary hematopoiesis seen in the spleen is a mechanism involved in the hematological maintenance reported during the acute phase of oral T. cruzi infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAlessandroMSantos_Julianademeis_etal_IOC_2022.pdfAlessandroMSantos_Julianademeis_etal_IOC_2022.pdfapplication/pdf4664644https://www.arca.fiocruz.br/bitstream/icict/52335/2/AlessandroMSantos_Julianademeis_etal_IOC_2022.pdfa377e8f36d6e9f43d924cae467752871MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52335/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/523352022-06-24 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dc.title.pt_BR.fl_str_mv Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
title Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
spellingShingle Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
Santos, Alessandro Marins dos
Trypanosoma cruzi
Infecção oral
Medula óssea
Timo
Hematopoiese
Células-tronco hematopoiéticas
Hematopoiese extramedular
Fluxo multiparamétrico
Trypanosoma cruzi
Oral infection
Bone marrow
Thymus
Hematopoiesis
Hematopoietic stem cells
Extramedullary hematopoiesis
Multiparametric flow
title_short Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
title_full Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
title_fullStr Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
title_full_unstemmed Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
title_sort Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis
author Santos, Alessandro Marins dos
author_facet Santos, Alessandro Marins dos
Silva, Jackline de Paula Ayres
Antunes, Dina
Moreira, Carlos José de Carvalho
Machado, Marcelo Pelajo
Alfaro, David
Zapata, Agustin G.
Bonomo, Adriana Cesar
Savino, Wilson
Meis, Juliana de
Oliveira, Désio Aurélio Farias de
author_role author
author2 Silva, Jackline de Paula Ayres
Antunes, Dina
Moreira, Carlos José de Carvalho
Machado, Marcelo Pelajo
Alfaro, David
Zapata, Agustin G.
Bonomo, Adriana Cesar
Savino, Wilson
Meis, Juliana de
Oliveira, Désio Aurélio Farias de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Alessandro Marins dos
Silva, Jackline de Paula Ayres
Antunes, Dina
Moreira, Carlos José de Carvalho
Machado, Marcelo Pelajo
Alfaro, David
Zapata, Agustin G.
Bonomo, Adriana Cesar
Savino, Wilson
Meis, Juliana de
Oliveira, Désio Aurélio Farias de
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Infecção oral
Medula óssea
Timo
Hematopoiese
Células-tronco hematopoiéticas
Hematopoiese extramedular
Fluxo multiparamétrico
topic Trypanosoma cruzi
Infecção oral
Medula óssea
Timo
Hematopoiese
Células-tronco hematopoiéticas
Hematopoiese extramedular
Fluxo multiparamétrico
Trypanosoma cruzi
Oral infection
Bone marrow
Thymus
Hematopoiesis
Hematopoietic stem cells
Extramedullary hematopoiesis
Multiparametric flow
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Oral infection
Bone marrow
Thymus
Hematopoiesis
Hematopoietic stem cells
Extramedullary hematopoiesis
Multiparametric flow
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-04-18T20:11:36Z
dc.date.available.fl_str_mv 2022-04-18T20:11:36Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Alessandro Martins dos et al. Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis. Frontiers in Cellular and Infection Microbiology, v. 12, Article 800395, p. 1-12, Mar. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/52335
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2022.800395
identifier_str_mv SANTOS, Alessandro Martins dos et al. Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis. Frontiers in Cellular and Infection Microbiology, v. 12, Article 800395, p. 1-12, Mar. 2022.
2235-2988
10.3389/fcimb.2022.800395
url https://www.arca.fiocruz.br/handle/icict/52335
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/52335/2/AlessandroMSantos_Julianademeis_etal_IOC_2022.pdf
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