Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals

Detalhes bibliográficos
Autor(a) principal: Lima, Luciane Mota
Data de Publicação: 2017
Outros Autores: Cardoso, Luciana Santos, Santos, Silvane Braga, Oliveira, Ricardo Riccio, Oliveira, Sérgio Costa, Góes, Alfredo Miranda, Loukas, Alex, Araujo, Maria Ilma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17949
Resumo: FundingCNPQ (Universal 479417/2008 3), NIH (R01AI079238A).
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spelling Lima, Luciane MotaCardoso, Luciana SantosSantos, Silvane BragaOliveira, Ricardo RiccioOliveira, Sérgio CostaGóes, Alfredo MirandaLoukas, AlexAraujo, Maria Ilma2017-03-06T19:04:37Z2017-03-06T19:04:37Z2017LIMA, L. M. et al. Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals. Acta Tropica, v. 167, p. 157–162, 2017.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/1794910.1016/j.actatropica.2016.12.030FundingCNPQ (Universal 479417/2008 3), NIH (R01AI079238A).Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilHospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. INCT-DT. CNPq/MCT. Salvador, BA, BrasilHospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilHospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilAustralian Institute of Tropical Health and Medicine, James Cook University, Queensland, AustraliaHospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Escola Baiana de Medicina e Saúde Pública, Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. INCT-DT. CNPq/MCT. Salvador, BA, BrasilHTLV-1 is the causal agent of Adult T cell Leukemia/lymphoma (ATLL) and HTLV-1-associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP). The immune response to HTLV-1-infection is polarized to the Th1-type, and the presence of CXCL9/CXCL10 chemokines may lead to an increase in the recruitment of pro-inflammatory molecules in spinal cord tissue, contributing to the damage observed in the development of HAM/TSP. It has been observed that in chronic helminth-infections, such as schistosomiasis, there is a deviation toward the Th2/regulatory immune response. Objective: To evaluate the ability of Schistosoma spp. proteins to decrease the in vitro CXCL9 and CXCL10 production by PBMC of HTLV-1-infected individuals. Methods: The Schistosoma proteins rSm29, rSh-TSP-2 and PIII were added to PBMC cultures of HTLV-1- infected individuals and the levels of chemokines in the supernatants were measured using a sandwich ELISA method. Results: The addition of rSm29 to the cultures resulted in decreased production of CXCL9 in all the analyzed individuals and HAM/TSP group (18167 ± 9727 pg/mL, p = 0.044; 20237 ± 6023 pg/mL, p = 0.028, respectively) compared to the levels in unstimulated cultures (19745 ± 9729 pg/mL; 25078 ± 2392 pg/mL, respectively). The addition of rSh-TSP-2 decreased the production of CXCL9 in all studied individuals and carriers group (16136 ± 9233 pg/mL, p = 0.031; 13977 ± 8857 pg/mL, p = 0.026) vs unstimulated cultures (19745 ± 9729 pg/mL; 18121 ± 10508 pg/mL, respectively). Addition of PIII did not alter the results. There was no significant change in the levels of CXCL10 by the addition of the studied proteins. Conclusion: The Schistosoma proteins used in this study were able to down modulate the production of CXCL9, a chemokine associated with the inflammatory process in HTLV-1-infection.engElsevierHTLV-1SchistosomaProteínasCXCL9CXCL10HTLV-1Schistosoma proteinsCXCL9CXCL10Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individualsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17949/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLima LM Schistosoma antigens....pdfLima LM Schistosoma antigens....pdfapplication/pdf782800https://www.arca.fiocruz.br/bitstream/icict/17949/2/Lima%20LM%20Schistosoma%20antigens....pdf8b5adbb94e252597edcd79c3d658006aMD52TEXTLima LM Schistosoma antigens....pdf.txtLima 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dc.title.pt_BR.fl_str_mv Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
title Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
spellingShingle Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
Lima, Luciane Mota
HTLV-1
Schistosoma
Proteínas
CXCL9
CXCL10
HTLV-1
Schistosoma proteins
CXCL9
CXCL10
title_short Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
title_full Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
title_fullStr Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
title_full_unstemmed Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
title_sort Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals
author Lima, Luciane Mota
author_facet Lima, Luciane Mota
Cardoso, Luciana Santos
Santos, Silvane Braga
Oliveira, Ricardo Riccio
Oliveira, Sérgio Costa
Góes, Alfredo Miranda
Loukas, Alex
Araujo, Maria Ilma
author_role author
author2 Cardoso, Luciana Santos
Santos, Silvane Braga
Oliveira, Ricardo Riccio
Oliveira, Sérgio Costa
Góes, Alfredo Miranda
Loukas, Alex
Araujo, Maria Ilma
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Luciane Mota
Cardoso, Luciana Santos
Santos, Silvane Braga
Oliveira, Ricardo Riccio
Oliveira, Sérgio Costa
Góes, Alfredo Miranda
Loukas, Alex
Araujo, Maria Ilma
dc.subject.other.pt_BR.fl_str_mv HTLV-1
Schistosoma
Proteínas
CXCL9
CXCL10
topic HTLV-1
Schistosoma
Proteínas
CXCL9
CXCL10
HTLV-1
Schistosoma proteins
CXCL9
CXCL10
dc.subject.en.pt_BR.fl_str_mv HTLV-1
Schistosoma proteins
CXCL9
CXCL10
description FundingCNPQ (Universal 479417/2008 3), NIH (R01AI079238A).
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-03-06T19:04:37Z
dc.date.available.fl_str_mv 2017-03-06T19:04:37Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, L. M. et al. Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals. Acta Tropica, v. 167, p. 157–162, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17949
dc.identifier.issn.pt_BR.fl_str_mv 0001-706X
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2016.12.030
identifier_str_mv LIMA, L. M. et al. Schistosoma antigens downregulate CXCL9 production by PBMC of HTLV-1-infected individuals. Acta Tropica, v. 167, p. 157–162, 2017.
0001-706X
10.1016/j.actatropica.2016.12.030
url https://www.arca.fiocruz.br/handle/icict/17949
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/17949/2/Lima%20LM%20Schistosoma%20antigens....pdf
https://www.arca.fiocruz.br/bitstream/icict/17949/3/Lima%20LM%20Schistosoma%20antigens....pdf.txt
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